Phân lập vi khuẩn tổng hợp enzyme agarase từ nước biển và xác định hoạt tính của enzyme
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Nghiên cứu này nhằm mục tiêu phân lập vi khuẩn có khả năng tổng hợp enzyme agarase từ nước biển và xác định hoạt tính của enzyme. Từ 8 mẫu nước biển thu thập tại các địa điểm khác nhau ở tỉnh Bà Rịa – Vũng Tàu, đã phân phập được 21 chủng vi khuẩn có khả năng phân giải agar trên đĩa thạch với đường kính vòng phân giải dao động từ 4,0 đến 7,0 cm sau 2 ngày ủ ở nhiệt độ phòng. Năm chủng vi khuẩn (M1, M5, M7, M62B, và M71) tạo đường kính vòng phân giải lớn nhất có hoạt độ enzyme agarase thô được xác định trong khoảng 0,15 – 0,22 U/mL khi phản ứng với cơ chất agarose, và có trình tự 16S rDNA tương đồng (> 96%) với chi Vibrio. Trong đó, chủng vi khuẩn M71 có hoạt tính agarase cao nhất và được dùng để đánh giá khả năng phân giải rong biển. Sự thủy phân rong đỏ Gracilaria bằng dịch enzyme thô của chủng M71 ở nồng độ 5% (v/v) giải phóng 915 915 µM/mL đường khử sau 24 giờ ủ ở 40oC.
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