Phan Vũ Hải , Hoàng Thị Anh Phương * , Tạ Đặng Ý Vi & Nguyễn Xuân Hòa

* Correspondence: Hoàng Thị Anh Phương (email: htaphuong@ttn.edu.vn)

Main Article Content

Tóm tắt

Nghiên cứu này đánh giá khả năng tiền sinh học của chiết xuất hành tím (Allium cepa L.) và ứng dụng của nó trong chế phẩm sinh học làm chất phụ gia thức ăn cho gia cầm. Kết quả cho thấy chiết xuất hành tím chứa các hợp chất có tiềm năng prebiotic với hàm lượng cao của Fructooligosacarit, Quercetin 3,40-diglucoside, Quercetin 40-monoglucoside và Quercetin aglycone, thúc đẩy sự tăng trưởng của cả 3 chủng L. plantarum 1582, L. plantarum WCFS1 và L. plantarum JDM1, đặc biệt ở nồng độ pha loãng 40%. Chủng L. plantarum 1582 thể hiện đặc tính probiotic vượt trội (khả năng kháng axit 8,97 log CFU/g, muối mật 10,42 log CFU/g, nhiệt độ 7,51 - 8,10 log CFU/g, duy trì mật độ tế bào cao trong cơ quan tiêu hoá sau 24 - 72 giờ 4,93 - 5,96 log CFU/g) - đã được sử dụng trong thử nghiệm chế phẩm lên men hành tím (HTLM). Bổ sung HTLM theo đường uống cho gà thịt cho thấy L. plantarum 1582 đã tồn tại tốt trong đường tiêu hóa, đặc biệt ở ruột non và manh tràng trong 24 - 48 giờ. Từ kết quả nghiên cứu đạt được cho thấy chế phẩm HTLM có tiềm năng sử dụng như một chất phụ gia thức ăn trong chăn nuôi gà thịt. Tuy nhiên, cần có thêm các nghiên cứu in vivo để đánh giá hiệu quả của HTLM này trong điều kiện thực tế.

Từ khóa: Hành tím, Lactobacillus plantarum, Lên men, Muối mật, Nhiệt độ

Article Details

Tài liệu tham khảo

Altuntas, S., & Korukluoglu, M. (2019). Growth and effect of garlic (Allium sativum) on selected beneficial bacteria. Food Science and Technology 39(4), 897-904. https://doi.org/10.1590/fst.10618.

Damayanti, E., Istiqomah, L., Saragih, J. E., & Purwoko, T. (2017). Characterization of lactic acid bacteria as poultry probiotic candidates with aflatoxin B1 binding activities. IOP Conference Series: Earth and Environmental Science 101(1), 1-8. https://doi.org/10.1088/1755-1315/101/1/012030.

Diriba-Shiferaw, G., Nigussie-Dechassa, R., Kebede, W., Getachew, T., & Sharma, J. J. (2013). Growth and nutrients content and uptake of garlic (Allium sativum L.) as influenced by different types of fertilizers and soils. Science, Technology and Arts Research Journal 2(3), 35-50. https://doi.org/10.4314/star.v2i3.98727.

Fernandes, L., Casal, S., Pereira, J. A., Saraiva, J. A., & Ramalhosa, E. (2017). Edible flowers: A review of the nutritional, antioxidant, antimicrobial properties and effects on human health. Journal of Food Composition and Analysis 60, 38-50. https://doi.org/10.1016/j.jfca.2017.03.017.

Gilchrist, M. J., Greko, C., Wallinga, D. B., Beran, G. W., Riley, D. G., & Thorne, P. S. (2007). The potential role of concentrated animal feeding operations in infectious disease epidemics and antibiotic resistance. Environmental Health Perspectives 115(2), 313-316. https://doi.org/10.1289/ehp.8837.

Gupta, A., & Sharma, N. (2017). Characterization of potential probiotic lactic acid bacteriaPediococcus acidilactici Ch-2 isolated from Chuli-A traditional apricot product of Himalayan region for the production of novel bioactive compounds with special therapeutic properties. Journal of Food: Microbiology, Safety and Hygiene 2(1), 1-11. https://doi.org/10.4172/2476-2059.1000119.

Hill, D., Sugrue, I., Tobin, C., Hill, C., Stanton, C., & Ross, R. P. (2018). The Lactobacillus casei group: History and health related applications. Frontiers in Microbiology 9, 1-12. https://doi.org/10.3389/fmicb.2018.02107.

Jiang, T., Li, H., Han, G., Singh, B., Kang, S. K., Bok, J. D., Kim, D. D., Hong, Z. S., Choi, Y. J., & Cho, C. (2017). Oral delivery of probiotics in poultry using ph-sensitive tablets. Journal of Microbiology and Biotechnology 27(4), 739-746. https://doi.org/10.4014/jmb.1606.06071.

Kareem, K. Y., Loh, T. C., Foo, H. L., Asmara, S. A., Akit, H., Abdulla, N. R., & FoongOoi, M. (2015). Carcass, meat and bone quality of broiler chickens fed with postbiotic and prebiotic combinations. International Journal of Probiotics and Prebiotics 10(1), 23-30.

Kumar, M., Barbhai, M. D., Hasan, M., Punia, S., Dhumal, S., Radha, Rais, N., Chandran, D., Pandiselvam, R., Kothakota, A., Tomar, M., Satankar, V., Senapathy, M., Anitha, T., Dey, A., Sayed, A. A. S., Gadallah, F. M., Amarowicz, R., & Mekhemar, M. (2022). Onion (Allium cepa L.) peels: A review on bioactive compounds and biomedical activities. Biomedicine and Pharmacotherapy 146, 112498. https://doi.org/10.1016/j.biopha.2021.112498.

Latorre, J. D., Hernandez-Velasco, X., Kallapura, G., Menconi, A., Pumford, N. R., Morgan, M. J., Layton, S. L., Bielke, L. R., Hargis, B. M., & Téllez, G. (2014). Evaluation of germination, distribution, and persistence of Bacillus subtilis spores through the gastrointestinal tract of chickens. Poultry Science 93(7), 1793-1800. https://doi.org/10.3382/ps.2013-03809.

Ma, J., Xu, C., Liu, F., Hou, J., Shao, H., & Yu, W. (2021). Stress adaptation and cross-protection of Lactobacillus plantarum KLDS 1.0628. CyTA–Journal of Food 19(1), 72-80. https://doi.org/10.1080/19476337.2020.1859619.

Mallappa, R. H., Singh, D. K., Rokana, N., Pradhan, D., Batish, V. K., & Grover, S. (2019). Screening and selection of probiotic Lactobacillus strains of Indian gut origin based on assessment of desired probiotic attributes combined with principal component and heatmap analysis. Food Science and Technology 105, 272-281. https://doi.org/10.1016/j.lwt.2019.02.002.

Mangisah, I., Yunianto, V. D., Sumarsih, S., & Sugiharto, S. (2021). Supplementation of garlic powder and Lactobacillus casei to improve nutrient digestibility, physiological conditions, and performance of broiler during starter phase. Journal of The Indonesian Tropical Animal Agriculture 46(4), 336-346. https://doi.org/10.14710/jitaa.46.4.336-346.

Mansouri, E. (2016). Viability of Bifidobacterium bifidum and Escherichia coli in versus prebiotic effects of Jerusalem artichoke (Helianthus tuberosus). Zahedan Journal of Research in Medical Sciences 18(11) 1-17. https://doi.org/10.17795/zjrms-3771.

Masalam, M. S. B., Bahieldin, A., Alharbi, M. G., AlMasaudi, S., Al-Jaouni, S. K., Harakeh, S. M., & Al-Hindi, R. R. (2018). Isolation, molecular characterization and probiotic potential of lactic acid bacteria in Saudi raw and fermented milk. Evidence-Based Complementary and Alternative Medicine 2018, 1-12. https://doi.org/10.1155/2018/7970463.

Myers, S. R., Hawrelak, J., & Cattley, T. (2009). Essential oils in the treatment of intestinal dysbiosis: A preliminary in vitro study. Alternative Medicine Review 14(4), 380-384.

O’Flaherty, S., Briner Crawley, A., Theriot, C. M., & Barrangou, R. (2018). The Lactobacillus bile salt hydrolase repertoire reveals niche-specific adaptation. MSphere 3(3), e00140-18. https://doi.org/10.1128/msphere.00140-18.

Pop, C., Suharoschi, R., & Pop, O. L. (2021). Dietary fiber and prebiotic compounds in fruits and vegetables food waste. Sustainability 13, 2-18. https://doi.org/10.3390/su13137219.

Saad, N., Delattre, C., Urdaci, M., Schmitter, J. M., & Bressollier, P. (2013). An overview of the last advances in probiotic and prebiotic field. LWTFood Science and Technology 50(1), 1-16. https://doi.org/10.1016/j.lwt.2012.05.014.

Sagar, N. A., Pareek, S., Benkeblia, N., & Xiao, J. (2022). Onion (Allium cepa L.) bioactives: Chemistry, pharmacotherapeutic functions, and industrial applications. Food Frontiers 3(3), 380-412. https://doi.org/10.1002/fft2.135.

Sanches Lopes, S. M., Francisco, M. G., Higashi, B., de Almeida, R. T. R., Krausová, G., Pilau, E. J., Gonçalves, J. E., Gonçalves, R. A. C., & Oliveira, A. J. B. (2016). Chemical characterization and prebiotic activity of fructo-oligosaccharides from Stevia rebaudiana (Bertoni) roots and in vitro adventitious root cultures. Carbohydrate Polymers152, 718-725. https://doi.org/10.1016/j.carbpol.2016.07.043.

Schloss, P. D. (2014). An integrated view of the skin microbiome. Nature 514(7520), 44-45. https://doi.org/10.1038/514044a.

Souza, L., Araujo, D., Stefani, L., Giometti, I., Cruz-Polycarpo, V., Polycarpo, G., & Mfc, B. (2018). Probiotics on performance, intestinal morphology and carcass characteristics of broiler chickens raised with lower or higher environmental challenge. Austral Journal of Veterinary Sciences 50(1), 35-41. http://dx.doi.org/10.4067/S0719-81322018000100107.

Sunu, P., Sunarti, D., Mahfudz, L.D., & Yunianto, V.D. (2019). Prebiotic activity of garlic (Allium sativum) extract on Lactobacillus acidophilus. Veterinary World 12(12), 2046-2051. https://doi.org/10.14202/vetworld.2019.2046-2051.

Vecchione, A., Celandroni, F., Mazzantini, D., Senesi, S., Lupetti, A., & Ghelardi, E. (2018). Compositional quality and potential gastrointestinal behavior of probiotic products commercialized in Italy. Frontiers in Medicine 5, 1-9.

VS (Vietnam Standards). (2017). Standard No. TCVN 11892-1:2017 dated on October 17th, 2017. Good agricultural practices (Vietgap) - Part 1: Crop production. Retrieved July 10, 2024, from http://www.vietgap.com/pic/files/tcvn-11892-1-2017.pdf.

VS (Vietnam Standards). (2007). Standard No. TCVN 6261:2007 dated on June 19th, 2007. Milk - enumeration of colony-forming units of psychrotrophic microorganisms - colony-count technique at 6.5oC. Retrieved July 10, 2024, from https://tieuchuan.vsqi.gov.vn/tieuchuan/view?sohieu=TCVN+6261%3A2007.

Wang, L., Zhang, H., Rehman, M. U., Mehmood, K., Jiang, X., Iqbal, M., Tong, X., Gao, X., & Li, J. (2018). Antibacterial activity of Lactobacillus plantarum isolated from Tibetan yaks. Microbial Pathogenesis 115, 293-298. https://doi.org/10.1016/j.micpath.2017.12.077.

Wilson, E. A., & Demmig‐Adams, B. (2007). Antioxidant, anti‐inflammatory, and antimicrobial properties of garlic and onions. Nutrition and Food Science 37(3), 178-183. https://doi.org/10.1108/00346650710749071.

Yadav, S., Trivedi, N. A., & Bhatt, J. D. (2015). Antimicrobial activity of fresh garlic juice: An in vitro study. An International Quarterly Journal of Research in Ayurveda 36(2), 203-207. https://doi.org/10.4103/0974-8520.175548.