Improving propagation of the rare plant Huperzia squarrosa using cuttings and in vitro techniques
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The tassel fern, Huperzia squarrosa, is a rare and medicinally valuable plant known for containing Huperzine A. It propagates naturally through spores, rhizomes, cuttings, and clump division, but with a slow multiplication rate. This study aimed to optimize propagation conditions for H. squarrosa using stem cuttings and in vitro culture techniques to support its preservation and development. Apical and stem cuttings were treated with varying concentrations of indole-3-butyric acid (IBA) and naphthaleneacetic acid (NAA) before being planted in a substrate of coir dust, charcoal dust, and burnt rice husk (3:2:2). Apical cuttings treated with 1500 ppm IBA for 30 min showed the highest rooting success, identifying this method as optimal for propagation. Additionally, surface sterilization with a 40% bleach solution, followed by antibiotic treatment, achieved a 73.8% clean sample rate. In vitro culturing on ¼ MS (Murashige and Skoog) medium resulted in 70% survival and 55% rooting after 60 days. The highest callus formation rate (13.3%) was achieved with 0.01 mg/L IBA and 0.3 mg/L Kinetin, while the addition of 3 mg/L Glutamine did not significantly enhance callus induction. Ongoing research focuses on enhancing complete plant regeneration and improving the efficiency of in vitro propagation for H. squarrosa.
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Ferreira, A., Rodrigues, M., Fortuna, A., Falcão, A., & Alves, G. (2016). Huperzine A from Huperzia serrata: A review of its sources, chemistry, pharmacology, and toxicology. Phytochemistry Reviews 15(1), 51-85. https://doi.org/10.1007/s11101-014-9384-y.
Ho, H. T., Le, A. T. L., Nguyen, T. D., Ngo, L. T. T., Ton, D. T. H., & Le, T. T. B. (2022). In vitro shoot multiplication and the content of huperzine A in in vitro cultured Huperzia serrata (Thunb. Ex Murray) Trevis plants. Academic Journal of Biology 44(1), 145-153. https://doi.org/10.15625/2615-9023/16207.
Katakawa, K., Mito, H., Kogure, N., Kitajima, M., Wongseripipatana, S., Arisawa, M., & Takayama, H. (2011). Ten new fawcettimine-related alkaloids from three species of Lycopodium. Tetrahedron 67(35), 6561-6567. https://doi.org/10.1016/j.tet.2011.05.107.
Le, A. T. L., Bui, A. T., Giang, S. X., Ton, D. T. H., Ho, H. T., Nguyen, T. D., & Le, T. T. B. (2019). A study on vegetative propagation of Huperzia serrata by cuttings in Sa Pa, Lao Cai. Academic Journal of Biology 41(3), 107-113. https://doi.org/10.15625/0866-7160/v41n3.13921.
Le, A. T. L. (2021). Study on some biological characteristics and propagation of Huperzia serrata (Thunb. ex-Murray) Trevis collected in Lao Cai and Lam Dong (Unpublished doctoral dissertation). Vietnam Academy of Science and Technology, Ha Noi, Vietnam.
Long, H., Li, Q., Li, L., & Huang, L. (2014). Study on cutting and gemmae propagation of Huperzia serrata. Journal of Chinese Medicinal Materials 37(7), 1115-1121.
Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Plant Physiology 15, 473-497.
Nguyen, C. N. (2020). Research on the standardization of acetylcholinesterase inhibitory compounds from some species of the Huperziaceae family (Unpublished doctoral dissertation). Ho Chi Minh City University of Medicine and Pharmacy, Ho Chi Minh City, Vietnam.
Pham, H. H. (1999). An illustration of flora of Vietnam - Vol. 1. Ho Chi Minh City, Vietnam: Tre Publishing House.
Szypuła, W., Pietrosiuk, A., Suchocki, P., Olszowska, O., Furmanowa, M., & Kazimierska, O. (2005). Somatic embryogenesis and in vitro culture of Huperzia selago shoots as a potential source of huperzine A. Plant Science 168(6), 1443-1452. https://doi.org/10.1016/j.plantsci.2004.12.021.
Tran, H. T. T., Hoang, H. N., Nguyen, T. T., Le, P. V., Ngo, T. T., & Khuat, T. H. (2019). The effect of culture medium on the in vitro propagation of Huperzia squarrosa (G. Forst.) Trev. The Journal of Agriculture and Rural Development 18, 134-141.
Upadhyay, S. D., Ahmad, Y., Sharma, R. K., & Kohli, S. (2020). Huperzinea: Pharmacological and therapeutic potential. Pharmacognosy Communications 10(4), 146-149. http://dx.doi.org/10.5530/pc.2020.4.31.
Yang, Y., Xiang, J., Li, Y., & Ma, J. (2021b). Effects of mineral nutrient medium on the micropropagation of Huperzia serrata. Journal of Plant Physiology 51, 465-470.
Yang, Y. Y., Dai, F. L., Wu, C. D., Dong, M. L., Tu, S. Y., Xie, K. J., & Luo, D. X. (2021a). In vitro propagation, huperzine A content and antioxidant activity of three genotypic Huperzia serrata. Plants 10(6), 1112. https://doi.org/10.3390/plants10061112.
Yumkham, S. D., & Singh, P. K. (2012). A novel way for propagation of Huperzia squarrosa (G. Forst.) Trev. Notulae Scientia Biologicae 4(4), 27-31. https://doi.org/10.15835/nsb448202.
Yumkham, S. D., & Singh, P. K. (2011). Huperzia squarrosa (G. Forst.) Trevis (Lycopodiaceae) in Manipur: Taxonomy and biological aspects. Taiwania 56(2), 157-164. http://dx.doi.org/10.6165/tai.2011.56(2).157.
Zhang, C. J., Zhang, Y. H., Ye, L. Z., Ye, H. X., & Zhu, M. (2009). Preliminary report of Huperzia serrata nursery constructing and original habitat cutting. Journal of Sanming University 26(2), 194-198.
Zuo, J. D., Yang, K. Y., Xiang, Q. J., Zeng, Z. F., Li, J. Y., Yin, Q. H., Zou, C. Y., & Ma, J. (2010). Study on cutting seedling NFT culture technique of Huperzia serrata (Thunb.) Trev. Journal of Hubei Institute for Nationalities (Natural Sciences) 28(1), 18-21.