Effects of partial precipitation and freeze-drying on morphology and physicochemical properties of rice starch hydrolysates
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Agricultural bio-catalysis is of immense scientific interest due to its increasing importance in the efforts for more sustainable agriculture while optimizing environmental impacts. In our studies, native rice starch was hydrolyzed with various alpha-amylase concentrations (0, 0.1, 0.2, and 0.3% w/w of starch) at 50°C for 20 min; then purified by partial precipitation (PP) with organic solvents, or freeze-drying (FD) without further purification. The rice starch hydrolysates (RSH) produced by different methods (PP or FD) were determined for dextrose equivalent (DE), morphology, and some physicochemical properties including bulk density, moisture content, hygroscopicity, and water solubility. The results showed that at the same alpha-amylase treatment conditions, the RSH obtained by the PP method had lower DE values and production yields than those of RSH obtained by FD method. The FD-RSH had higher DE values, lower bulk densities and moisture contents, higher hygroscopicity and water solubility. In morphology, the PP-RSH (DE 10.2) had a larger particle size and more condensed microstructure compared to the FD-RSH of almost similar DE 13.5. These findings showed that the PP method resulted in lower-DE RSH with different morphological and physicochemical properties compared to those obtained by the FD method.
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