Nguyễn Đức Trọng , Lý Ngọc Thanh Xuân , Trần Chí Nhân , Mai Chí Bảo , Lê Hiền Minh Tâm , Phan Chấn Hiệp , Trần Trọng Khôi Nguyên , Lê Thị Mỹ Thu & Nguyễn Quốc Khương *

* Correspondence: Nguyễn Quốc Khương (email: nqkhuong@ctu.edu.vn)

Main Article Content

Tóm tắt

Nghiên cứu được thực hiện nhằm mục tiêu xác định tỷ lệ vật liệu và độ ẩm phù hợp để sản xuất phân hữu cơ từ thân, lá khóm bằng Trichoderma spp. (TC). Thí nghiệm 2 nhân tố được bố trí hoàn toàn ngẫu nhiên, nhân tố (A) là bổ sung nấm Trichoderma spp. phân hủy cellulose gồm (i) Không bổ sung, (ii) Tricho-ĐHCT, (iii) TC1, (iv) TC2, (v) TC3 và (vi) hỗn hợp 3 dòng TC1, TC2, TC3, nhân tố (B) là ẩm độ gồm: (i) 50%, (ii) 60% và (iii) 70%. Thí nghiệm được thực hiện trên 3 tỷ lệ vật liệu khác nhau (khóm:bã bùn mía:phân bò, với tỷ lệ 7:1,5:1,5 (KBB7); 8:1:1 (KBB8); 9:0,5:0,5 (KBB9)). Kết quả cho thấy vật liệu ủ KBB7 và KBB8 có hàm lượng P tổng số của nghiệm thức bổ sung hỗn hợp 3 dòng nấm Trichoderma spp. TC1, TC2 và TC3 cao hơn các nghiệm thức bổ sung dòng đơn Trichoderma spp. TC1, TC2, TC3, với 0,574 so với 0,389 - 0,463%; 0,507 so với 0,351 - 0,380%, và 0,422 so với 0,303 - 0,427% theo thứ tự. Tương tự, tỷ lệ C/N ở cả 3 tỷ lệ vật liệu KBB7, KBB8 và KBB9 nghiệm thức đối chứng có tỷ lệ C/N cao hơn nghiệm thức có bổ sung nấm Trichoderma spp. và nghiệm thức bổ sung Tricho-ĐHCT, lần lượt là 43,8 so với 28,3 - 35,1, 47,8 so với 31,6 - 35,2 và 54,8 so với 34,2 - 37,1. Hàm lượng C tổng số cũng cho kết quả tương tự. Tỷ lệ vật liệu 7:1,5:1,5 (KBB7) ở ẩm độ 50 - 60% phù hợp cho ủ phân hữu cơ.

Từ khóa: Phân hữu cơ, Phụ phẩm trồng khóm, Trichoderma spp., Ủ phân

Article Details

Tài liệu tham khảo

Alkarimiah, R., & Suja, F. (2019). Effects of technical factors towards achieving the thermophilic temperature stage in composting process and the benefits of closed rector system compared to conventional method - a mini review. Applied Ecology and Environmental Research 17(4), 9979-9996. https://doi.org/10.15666/aeer/1704_99799996.

Arora, M., & Kaur, A. (2019). Azolla pinnata, Aspergillus terreus, and Eisenia fetida for faster recycling of nutrients from wheat straw. Environmental Science and Pollution Research 26, 32624-32635. https://doi.org/10.1007/s11356-019-06362-x.

Berenguer, C. V., Perestrelo, R., Pereira, J. A., & Câmara, J. S. (2023). Management of agri-food waste based on thermochemical processes towards a circular bioeconomy concept: The case study of the Portuguese industry. Processes 11(10), 2870. https://doi.org/10.3390/pr11102870.

Bird, J. A., Horwath, W. R., Eagle, A. J., & van Kessel, C. (2001). Immobilization of fertilizer nitrogen in rice: effects of straw management practices. Soil Science Society of America Journal 65(4), 1143-1152. https://doi.org/10.2136/sssaj2001.6541143x.

Bolan, S., Hou, D., Wang, L., Hale, L., Egamberdieva, D., Tammeorg, P., Li, R., Wang, B., Xu, J., Wang, T., Sun, H., Padhye, L. P., Wang, H., Siddique, K. H. M., Rinklebe, J., Kirkham, M. B., & Bolan, N. (2023). The potential of biochar as a microbial carrier for agricultural and environmental applications. Science of The Total Environment 886, 63968. https://doi.org/10.1016/j.scitotenv.2023.163968.

Cassellis, M. E. R., Pardo, M. E. S., López, M. R., & Escobedo, R. M. (2014). Structural, physicochemical and functional properties of industrial residues of pineapple (Ananas comosus). Cellulose Chemistry and Technology 48(7-8), 633-641.

Deng, W., Zhang, A., Chen, S., He, X., Jin, L., Yu, X., Yang, S., Li, B., Fan, L., Ji, L., Pan, X., & Zou, L. (2020). Heavy metals, antibiotics and nutrients affect the bacterial community and resistance genes in chicken manure composting and fertilized soil. Journal of Environmental Management 257, 109980. https://doi.org/10.1016/j.jenvman.2019.109980.

Estrada-Bonilla, G. A., Durrer, A., & Cardoso, E. J. (2021). Use of compost and phosphatesolubilizing bacteria affect sugarcane mineral nutrition, phosphorus availability, and the soil bacterial community. Applied Soil Ecology 157, 103760. https://doi.org/10.1016/j.apsoil.2020.103760.

Feng, X., Sun, J., & Xie, Y. (2021). Degradation of Shanxi lignite by Trichoderma citrinoviride. Fuel 291, 120204. https://doi.org/10.1016/j.fuel.2021.120204.

Gao, X., Liu, W., Li, X., Zhang, W., Bu, S., & Wang, A. (2023). A novel fungal agent for straw returning to enhance straw decomposition and nutrients release. Environmental Technology and Innovation 30, 103064. https://doi.org/10.1016/j.eti.2023.103064.

Gong, Y. Z., Niu, Q. Y., Liu, Y. G., Dong, J., & Xia, M. M. (2022). Development of multifarious carrier materials and impact conditions of immobilised microbial technology for environmental remediation: A review. Environmental Pollution 314, 120232. https://doi.org/10.1016/j.envpol.2022.120232.

He, J., Zhu, N., Xu, Y., Wang, L., Zheng, J., & Li, X. (2022). The microbial mechanisms of enhanced humification by inoculation with Phanerochaete chrysosporium and Trichoderma longibrachiatum during biogas residues composting. Bioresource Technology 351, 126973. https://doi.org/10.1016/j.biortech.2022.126973.

Houba, V. J. G., Novozamsky, I., & der Lee, J. J. V. (1995). Influence of storage of plant samples on their chemical composition. Science of The Total Environment 176(1-3), 73-79. https://doi.org/10.1016/0048-9697(95)04831-M.

Ilias, G. N. M., Rahman, M. A., Molla, A. H., Begum, M. F., & Alam, M. F. (2005). Composting of municipal garbage by using Trichoderma - a new approach in context of Bangladesh. Bangladesh Journal of Genetics and Biotechnology 6(1), 75- 78.

Irawan, B., Jabbar, S. K., & Farisi, S. (2023). Application of Trichoderma sp. in pineapple biomass composting. Magna Scientia Advanced Biology and Pharmacy 9(02), 048-053. https://doi.org/10.30574/msabp.2023.9.2.0048.

Joos, L., Herren, G. L., Couvreur, M., Binnemans, I., Oni, F. E., Höfte, M., Debode, J., & Steel, H. (2020). Compost is a carrier medium for Trichoderma harzianum. BioControl 65, 737-749. https://doi.org/10.1007/s10526-020-10034-7.

Kapri, A., & Tewari, L. (2010). Phosphate solubilization potential and phosphatase activity of rhizospheric Trichoderma spp. Brazilian Journal of Microbiology 41, 787-795. https://doi.org/10.1590/S1517-83822010000300031.

Khan, M. S., Zaidi, A., Ahemad, M., Oves, M., & Wani, P. A. (2010). Plant growth promotion by phosphate solubilizing fungi–current perspective. Archives of Agronomy and Soil Science 56(1), 73-98. https://doi.org/10.1080/03650340902806469.

Li, R., Li, L., Huang, R., Sun, Y., Mei, X., Shen, B., & Shen, Q. (2014). Variations of culturable thermophilic microbe numbers and bacterial communities during the thermophilic phase of composting. World Journal of Microbiology and Biotechnology 30, 1737-1746. https://doi.org/10.1007/s11274-013-1596-4.

López-Mondéjar, R., Zühlke, D., Becher, D., Riedel, K., & Baldrian, P. (2016). Cellulose and hemicellulose decomposition by forest soil bacteria proceeds by the action of structurally variable enzymatic systems. Scientific Reports 6(1), 25279. https://doi.org/10.1038/srep25279.

Mahapatra, S., Ali, M. H., & Samal, K. (2022). Assessment of compost maturity-stability indices and recent development of composting bin. Energy Nexus 6, 100062. https://doi.org/10.1016/j.nexus.2022.100062.

Makan, A., Assobhei, O., & Mountadar, M. (2013). Effect of initial moisture content on the invessel composting under air pressure of organic fraction of municipal solid waste in Morocco. Iranian Journal of Environmental Health Science and Engineering 10, 1-9. https://doi.org/10.1186/1735-2746-10-1.

Meena, A. L., Karwal, M., Dutta, D., & Mishra, R. P. (2021). Composting: phases and factors responsible for efficient and improved composting. Agriculture and Food: e-Newsletter 1, 85-90.

MOST (Ministry of Science and Technology). (2000). TCVN 6642:2000 (ISO 10694:1995) issued on December 20, 2000. Soil quality - Determination of organic and total carbon after dry combustion (elemental analysis). Issued on December 20, 2000. Retrieved January 25, 2018, from https://tieuchuan.vsqi.gov.vn/tieuchuan/view?id=1418.

Negi, R., & Suthar, S. (2018). Degradation of paper mill wastewater sludge and cow dung by brownrot fungi Oligoporus placenta and earthworm (Eisenia fetida) during vermicomposting. Journal of Cleaner Production 201, 842-852. https://doi.org/10.1016/j.jclepro.2018.08.068.

Organo, N. D., Granada, S. M. J. M., Pineda, H. G. S., Sandro, J. M., Nguyen, V. H., & Gummert, M. (2022). Assessing the potential of a Trichoderma-based compost activator to hasten the decomposition of incorporated rice straw. Scientific Reports 12(1), 448. https://doi.org/10.1038/s41598-021-04430-y.

Pandit, N. P., Ahmad, N., & Maheshwari, S. K. (2012). Vermicomposting biotechnology an ecoloving approach for recycling of solid organic wastes into valuable biofertilizers. Journal of Biofertilizers and Biopesticides 3, 1-8. https://doi.org/10.4172/2155-6202.1000134.

Polman, E. M., Gruter, G. J. M., Parsons, J. R., & Tietema, A. (2021). Comparison of the aerobic biodegradation of biopolymers and the corresponding bioplastics: A review. Science of The Total Environment 753, 141953. https://doi.org/10.1016/j.scitotenv.2020.141953.

Rahman, A., Begum, M. F., Rahman, M., Bari, M. A., Illias, G. N. M., & Alam, M. F. (2011). Isolation and identification of Trichoderma species from different habitats and their use for bioconversion of solid waste. Turkish Journal of Biology 35(2), 183-194. https://doi.org/10.3906/biy-0901-21.

Ren, X., Jiao, M., Chen, X., Liu, T., Zhang, Y., & Zhang, Z. (2023). Role of bulking agents and additive on composting. In Pandey, A., Awasthi, M., & Zhang, Z. (Eds.), Current developments in biotechnology and bioengineering (127-142). Amsterdam, Netherlands: Elsevier. https://doi.org/10.1016/B978-0-323-91874-9.00015-2.

Sharma, N., Singh, J., Singh, B., & Malik, V. (2023). Improving the agronomic value of paddy straw using Trichoderma harzianum, Eisenia fetida and cow dung. Fermentation 9(7), 671. https://doi.org/10.3390/fermentation9070671.

Strakowska, J., Błaszczyk, L., & Chełkowski, J. (2014). The significance of cellulolytic enzymes produced by Trichoderma in opportunistic lifestyle of this fungus. Journal of Basic Microbiology 54(S1), S2-S13. https://doi.org/10.1002/jobm.201300206.

Sun, Q., Wu, D., Zhang, Z., Zhao, Y., Xie, X., Wu, J., Lu, W., & Wei, Z. (2017). Effect of cold-adapted microbial agent inoculation on enzyme activities during composting start-up at low temperature. Bioresource Technology 244, 635-640. https://doi.org/10.1016/j.biortech.2017.04.130.

Suthar, S., Pandey, B., Gusain, R., Gaur, R. Z., & Kumar, K. (2017). Nutrient changes and biodynamics of Eisenia fetida during vermicomposting of water lettuce (Pistia sp.) biomass: a noxious weed of aquatic system. Environmental Science and Pollution Research 24, 199-207. https://doi.org/10.1007/s11356-016-7771-4.

Wan, L., Wang, X., Cong, C., Li, J., Xu, Y., Li, X., Hou, F., Wu, Y., & Wang, L. (2020). Effect of inoculating microorganisms in chicken manure composting with maize straw. Bioresource Technology 301, 122730. https://doi.org/10.1016/j.biortech.2020.122730.

Wong, J. W. C., Mak, K. F., Chan, N. W., Lam, A., Fang, M., Zhou, L. X., Wu, Q. T., & Liao, X. D. (2001). Co-composting of soybean residues and leaves in Hong Kong. Bioresource Technology 76(2), 99-106. https://doi.org/10.1016/S0960-8524(00)00103-6.

Yeh, C. K., Lin, C., Shen, H. C., Cheruiyot, N. K., Camarillo, M. E., & Wang, C. L. (2020). Optimizing food waste composting parameters and evaluating heat generation. Applied Sciences 10(7), 2284. https://doi.org/10.3390/app10072284.

Zhao, X., He, X., Xi, B., Gao, R., Tan, W., Zhang, H., & Li, D. (2016). The evolution of water extractable organic matter and its association with microbial community dynamics during municipal solid waste composting. Waste Management 56, 79-87. https://doi.org/10.1016/j.wasman.2016.07.031.

Zhu, P., Shen, Y., Pan, X., Dong, B., Zhou, J., Zhang, W., & Li, X. (2021). Reducing odor emissions from feces aerobic composting: additives. RSC Advances 11(26), 15977-15988. https://doi.org/10.1039/D1RA00949D.