Ảnh hưởng của bổ sung chế phẩm sinh học trong ương ấu trùng cua biển (Scylla paramamosain estampador, 1949)
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Nghiên cứu này nhằm đánh giá ảnh hưởng của các loại chế phẩm sinh học lên tăng trưởng và tỷ lệ sống của ấu trùng cua biển (Scylla paramamosain). Thí nghiệm gồm 4 nghiệm thức: (i) Nghiệm thức đối chứng (không bổ sung chế phẩm sinh học), (ii) bổ sung L. acidophilus, (iii) bổ sung B. subtilis, (iv) bổ sung kết hợp L. acidophilus và B. subtilis, mỗi nghiệm thức được lặp lại 3 lần và bố trí hoàn toàn ngẫu nhiên. Ấu trùng được ương trong bể nhựa 60 L với mật độ 200 con/L, độ mặn 26 ppt và bể ương được thay 3 ngày/lần, với 25% thể tích nước. Trong suốt thời gian ương bể ương được bổ sung chế phẩm vi sinh mỗi 3 ngày/lần với mật độ 104 CFU/mL. Kết quả cho thấy mật độ vi khuẩn tổng cao nhất ở nghiệm thức bổ sung L. acidophilus (16,38 x 104 CFU/mL) khác biệt có ý nghĩa thống kê (P < 0,05) so với nghiệm thức đối chứng (6,37 x 104 CFU/mL). Mật độ vi khuẩn Vibrio spp. ở nghiệm thức đối chứng cao nhất (6,67 x 103 CFU/mL) khác biệt có ý nghĩa (P < 0,05) so với các nghiệm thức có bổ sung chế phẩm vi sinh. Chỉ số biến thái (LSI) của ấu trùng của các nghiệm thức sử dụng vi sinh khác biệt không có ý nghĩa thống kê giữa các nghiệm thức (P > 0,05). Tỷ lệ sống đến Cua1 thấp nhất ở nghiệm thức đối chứng (7,51%) khác biệt có ý nghĩa thống kê (P < 0,05) so với các nghiệm thức còn lại. Nghiệm thức bổ sung L. acidophilus cho tỷ lệ sống cao nhất (10,04%). Kết quả nghiên cứu này cho thấy bổ sung L. acidophilus trong ương ấu trùng cua biển đạt hiệu quả cao nhất.
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