Published: 2025-01-22

The resistance of some cassava lines/varieties to Sri Lanka cassava mosaic virus in Quang Binh province

Dong T. H. Tran, Phuc K. Le, Tho V. Le, Trang T. N. Vo, Tam N. Nguyen, & Thuy T. N. Truong
Electronic version: 22 Jan 2025 | DOI: 10.52997/jad.1.01.2025
Abstract | PDF (141.3K)

Abstract

Six lines or varieties of cassava, including three potential lines or varieties (HL-KM94, HL-S14, KM505-54) and three lines resistance to the Sri Lanka cassava mosaic virus (SLCMV) (HN1, HN5, and HN97), were evaluated in 2022 in Quang Binh province. The aim of this study was to select cassava lines/varieties with high yield, high starch content and resistance to SLCMV disease, suitable for local conditions. The experiment was designed in a randomized complete block design (RCBD), with 3 replications. The research results showed that the cassava lines/varieties used in the study had an average germination time of 16.7 - 22.0 days; the average height of the plant was 277.4 - 363.4 cm, and an average growth time of 277.4 - 363.4 cm and the average growth duration was from 272 - 300 days. The average tuber weight ranged from 190 - 850 g/tuber. The skin color of the tuber was from white to light - yellow and the tuber flesh was white (except HN97 which had yellow tuber flesh). Regarding SLCMV, the study showed that 3 cassava lines including HN1, HN5, and HN97 did not show any signs of SLCMV disease at the seedling stage and after 6 months planting, the SLCMV disease appeared at a low average rate of 3.1 - 5.2%. Based on these results, two cassava lines/varieties HN1 and HL-KM94 were selected for their good growth, resistance to SLCMV, and high fresh tuber yield of 48.0 - 54.9 tons/ha. Their harvest index ranged from 64.4 - 67% and they had starch content over 28%.

Influence of cultivation densities and harvesting rates on the environmental parameters, growth and productivity of eelgrass, Vallisneria spiralis

Kha V. Tran, & Thao T. T. Ngo
Electronic version: 22 Jan 2025 | DOI: 10.52997/jad.1.04.2025
Abstract | PDF (349.6K)

Abstract

This study was conducted to evaluate effects of planting density and harvest rate on the growth and yield of eelgrass (Vallisneria spiralis). Experiment 1 aimed to evaluate the effects of different planting densities including three treatments of eelgrass planting density with three repetitions: 1) 0.25 kg/m2; 2) 0.50 kg/m2 and 3) 0.75 kg/m2. The results showed that the growth rate (3210 mg/day) and increase in biomass (83 g) when planted at a density of 0.50 kg/m2 were similar to those when planted at a density of 0.75 kg/m2 (P > 0,05), however, the yield was high (0.896 kg/m2) when eelgrass was planted at a density of 0.75 kg/m2. Experiment 2 evaluated effects of different harvest rates, including 4 treatments and was also repeated 3 times per each treatment: No harvest and harvest 15, 25, 35% total biomass/time after every 15 days of planting. The results of the 15 and 25% harvest rates did not differ in growth and yield of eelgrass (P > 0.05), however, the 35% harvest rate clearly reduced growth rate, biomass increase and productivity of V. spiralis.

Antimicrobial resistance of Vibrio parahaemolyticus isolates from oyster sold in Thu Duc city, Ho Chi Minh City

Hue N. D. Truyen, Kha H. N. Nguyen, Phuong N. Ngo, Duyen T. T. Nguyen, & Nhung H. Pham
Electronic version: 22 Jan 2025 | DOI: 10.52997/jad.1.06.2025
Abstract | PDF (480.4K)

Abstract

This study was conducted to determine the rate of antibiotic resis tance in 75 Vibrio parahaemolyticus strains isolated from oysters sold in Thu Duc city, Ho Chi Minh City. The results of the anti biotic susceptibility test showed that all strains were sensitive to chloramphenicol and florfenicol, but resistant to ampicillin. The percentages of isolates resistance to streptomycin, tetracycline, trimethoprim/sulfamethoxazol, nalidixic acid, ciprofloxacin, enrofloxacin, nitrofurantoin were 56%; 4%; 4%; 4%; 22,7%; 38,7%, respectively. The percentage of multiple resistant isolates from two to six tested antibiotics was 96% and the multiple antibiotic resis tance (MAR) index ranged from 0.2 to 0.42.

Effects of probiotic supplementation in rearing mud crab larvae (Scylla paramamosain estampador, 1949)

Vu H. Le, Tuyen B. Cao, & Bac V. Nguyen
Electronic version: 22 Jan 2025 | DOI: 10.52997/jad.1.03.2025
Abstract | PDF (188.8K)

Abstract

The research was conducted to evaluate effects of probiotics supplementation on growth performance and survival rate of mud crab (Scylla paramamosain) larvae. The experiment was arranged in a completely randomized design with 4 diets supplemented with various probiotics: (i) Control (w/o supplementation), (ii) L. acidophilus, (iii) B. subtilis, and (iv) a mixture of L. acidophilus and B. subtilis. A total of 12 composite tanks were filled with 60 L sterilized saline water (salinity 26 ppt) with a stocking density of 200 larvae/L and water exchange was controlled every 3 days (25% of the water volume). The probiotics (104 CFU/mL) were added separately to the experimental tanks every 3 days over a culture period. The L. acidophilus supplementation resulted in significantly higher total probiotics density (16.38 x 104 CFU/mL) compared with the control (6.37 x 104 CFU/mL) (P < 0.05). Furthermore, the control showed the highest Vibrio spp. density (6.67 x 103 CFU/mL) which differed significantly (P < 0.05) from remaining treatments with probiotics supplementation. The larval stage index (LSI) of mud crab larvae in probiotic supplemented treatments did not differ significantly (P > 0.05). The control had the lowest Crablet 1 ’s survival rate (7.51%) which differed significantly from remaining treatments (P < 0.05). In contrast, the L. acidophilus-supplemented treatment achieved the highest survival rate (10.04%). In conclusion, the obtained results suggested that L. acidophilus supplementation was considered the most potential approach to mud crab larvae production.

Knowledge - attitude - practice of students of the Faculty of Animal Science and Veterinary Medicine, Nong Lam University, Ho Chi Minh City on the risk of zoonosis

Bao D. Truong, Nguyet T. T. Nguyen, Truc T. Nguyen, Nhi T. T. Nguyen, Nhi T. Y. Nguyen, Loan P. B. Tran, Thong Q. Le, & Dung T. T. Nguyen
Electronic version: 22 Jan 2025 | DOI: 10.52997/jad.1.02.2025
Abstract | PDF (184.5K)

Abstract

The zoonosis is a significant health issue for both the medical and veterinary fields. Students in these disciplines play crucial roles in preventing and controlling such diseases. This study aimed to understand the knowledge (K), attitudes (A), and practices (P) regarding the prevention and control of zoonotic diseases among students majoring in animal science and veterinary medicine at the Nong Lam University, Ho Chi Minh City. Two methods used in this cross-sectional study were KAP and Q surveys, conducted on 329 and 46 subjects, respectively. The results indicated that students exhibited good K, A, and P levels at 66%, 48%, and 60%, respectively. The academic year influenced the formation of knowledge and attitudes. Raising two different kinds of animals at home was a factor that influenced the attitudes. The number of information sources affected differences in attitudes and behaviors. Three distinct student clusters were formed based on KAP scores. Cluster 1 had lower scores in all three aspects than the population average, cluster 3 had higher KAP scores than the population average, and cluster 2 had higher KP but lower A than the population average. Two student opinion groups (SOGs) were formed through the Q methodology, where SOG 1 was more concerned about personal health, and SOG 2 was more concerned about animal health. Both groups mentioned interdisciplinary collaboration to address the issue of zoonotic diseases. The proportion of students with good KAP was relatively low, and factors influencing this proportion were identified in the study. Thus, this highlighted the need for additional solutions and enhancements from teachers and researchers with the One Health approach playing a crucial role.

Application of electroacupuncture in the treatment of hind limb movement disorders in dogs

Duyen T. H. Pham, Thong Q. Le, & Thuong T. Nguyen
Electronic version: 22 Jan 2025 | DOI: 10.52997/jad.1.05.2025
Abstract | PDF (1.1M)

Abstract

The objective of this study was to apply and evaluate the effectiveness of electroacupuncture in the treatment of hind limb movement disorders in dogs, the study was conducted from June 2023 to June 2024 at VSA Pet Clinic. The neurological examination assessed the spinal cord lesions such as urinary incontinence, fecal incontinence, hopping, examined and evaluated the animal’s conscious position sense, superficial pain perception and deep pain perception. Classification of hind limb movement disorders in dogs included weakness and paralysis. Using the Acupuncture Unit 20-needle electroacupuncture machine with a 6V power source, frequency 0 - 60 Hz. A total of 32 dogs with hind limb movement disorders had 28.13% of weakness and 71.87% of paralysis, including mixed breed dogs, bulldog, dachshund, poodle, domestic dogs, chihuahua, pomeranian and corgi. Lower motor nerve (LMN) lesions accounted for 46.87%, while upper motor nerve (UMN) lesions were 53.13% (P > 0.05). The results of Glasgow pain score, there was 28 dogs (87.5%) at scoring ≥ 6 points, the average pain scores were 9 points in paralysis cases, 6 points in weakness, 8.33 points in LMN lesions, and 8.00 points in UMN lesions. The effectiveness of treatment was 65.67%, with an average acupuncture time of 20 days, in which the recurrence rate was 14.28%. Electroacupuncture was highly effective and had a low recurrence rate in the treatment of hind limb motor neuropathy in dogs.

Evaluation of genetic diversity of mango (Mangifera indica L.) collected from Ba Den mountain, Tay Ninh province based on Simple Sequence Repeats markers

Anh T. Tran, Phong D. Tran, & Toan D. Pham
Electronic version: 22 Jan 2025 | DOI: 10.52997/jad.1.07.2025
Abstract | PDF (1.7M)

Abstract

Mango (Mangifera indica L.) is a perennial fruit tree and belongs to the Anacardiaceae family. In the Ba Den Mountain area of Tay Ninh province, the ancient mango trees are intercropped with other plants in an area of about 530 ha. The objective of this study was to evaluate the genetic diversity of mango trees collected from the Ba Den mountain in Tay Ninh province based on Simple Sequence Repeats (SSR) markers for the conservation of genetic resources. A total of 9 SSR markers were used to evaluate the genetic diversity of 30 mango cultivars. The results displayed a total of 21 amplified fragments, averaging 2.33 fragments per primer. Of these, 20 out of 21 fragments were polymorphism, resulting in a polymorphic percentage of 94.4%. Molecular sizes ranged from 200 bp to 330 bp. Cluster analysis revealed high genetic diversity among the 30 mango samples, with genetic polymorphism levels ranging from 0 to 0.43 and an average genetic polymorphic distance of 0.29. The dendrogram was divided into 3 main groups, in which group I consisted of 18 mango samples, group II included 7 samples and group III comprised 5 samples. The results achieved could be useful information in assessing genetic diversity for genetic conservation and developing mango varieties in the future.