The objective of this study was to investigate uterine microbiota in postpartum dairy cows and barn environment by quantitative PCR and to evaluate the correlation between the microbiota in the uterus and barn environment. The study was carried out in 2 seasons of the year: summer (June to August 2017) and winter (October 2017 to March 2018) on Holstein dairy cow farm, Okayama Livestock Research Institute, Japan. A total of 116 samples, including 68 uterine and fecal samples, were collected from 9 cows in summer and 8 cows in winter, at 1 and 2 months after calving. Additionally, 48 samples of barn environment including airborne dust, bedding, feed and water samples were collected 6 times throughout each season. The quantative PCR results showed significant differences (P < 0.05) in the uterine and fecal microbiota of dairy cows at 1 and 2 months after calving. In summer, total bacteria at 2 months postpartum were higher than those at 1 month after calving, while total bacteria were the same in winter (P > 0.05). Bacteroidetes and Firmicutes in uterus and feces between 1 and 2 months after calving were not significantly different in both summer and winter (P > 0.05). The populations of Bacteroidetes, Firmicutes, and total bacteria were found to be highest in bedding compared to those in feed, airborne dust, and water from the barn environment (P < 0.05). Bacteroidetes, Firmicutes and total bacteria in uterus were closely related with those in bedding in summer, however they had the negative correlation with the microbiota of barn environment in winter, especially with fecal microbiota.

The study aimed to determine the prevalence, symptoms and lesions of coccidiosis in chickens experimentally infected with coccidial oocysts at low doses. Chickens (n = 40, 14 days old, coded MD02 from Minh Du company) were randomly assigned to four equal groups. Group 1, group 2 and group 3 chickens were orally inoculated with 1, 10 & 300 isolated oocysts of Eimeria species, respectively. Meanwhile, the chickens in group 4 were inoculated with phosphate-buffered saline as the control. Chickens in each treatment were raised individually. Fecal samples were collected individually every day after infection for oocysts counting. The results showed that chickens were infected with coccidial oocysts and had typical symptoms and lesions, even chickens were inoculated with one oocyst only. The prevalence of coccidiosis increased gradually with increasing levels of infectious dose, reaching 100% in group 3. The period of oocyst shedding was from 4 to 10 days post-inoculation. The number of oocysts was highest on day 6 post-inoculation. The oocyst counts were positively correlated with the infectious dose. Symptoms of anorexia, lethargy, wing drop and whitish, watery and bloody diarrhea were observed in infected chickens. The major lesions recorded were hemorrhages in the ceca (75 - 100%) and small intestines (100%).

This study focused on extracting flavonoids, a group of natural compounds with high biological activity, from Siam weed (Chromolaena odorata). Various extraction conditions were investigated including the ratio of raw material to solvent, temperature, and extraction time. The highest flavonoid content was obtained when using ethanol with a concentration of 70% and a raw material-to-solvent ratio of 1:200 g/mL, at a temperature of 50oC for 2 h. Additionally, antibacterial activity test revealed that both the extract and gel (supplemented with the extract) were effective in limiting the growth of three bacteria strains including Staphylococcus aureus, Salmonella, and Escherichia coli. Therefore, this study suggests that this wild plant species may be a good source of natural antibacterial agents.

The objective of the research was to evaluate the current situation of land use right transfer in Ba Ria city and proposed solutions to improve the effectiveness of the land use right transfer process. The study collected secondary data and conducted interviews with land users, real estate businesses, and individuals and organizations involved in the land use right transfer process in Ba Ria city. The study results showed that the number of land use right transfer cases increased significantly from 2016 to 2019, and the quality and attitude of the land use right transfer procedures were improved. However, there were still many limitations that need to be addressed, such as slow processing of documents, delayed issuance of certificates, inadequate public services, multiple trips required to complete procedures, limited and formalized public information, confusion, and complex financial and legal regulations regarding land use rights, causing difficulties for the land users to prepare their documents.

Tien Giang is one of the Mekong Delta's largest jackfruit-growing provinces, with a total cultivation area of jackfruit of approximately 13,141.09 ha. Bronzing of jackfruit appeared on Thai jackfruit and caused significant damage to growers, however, the information about the disease is still limited. In this study, we assessed the status of bronzing diseases on Thai jackfruit in Tien Giang province via direct interviews with farmers, mapped the disease distribution by Google Earth, and recorded the disease appearance on Thai jackfruit during the year's two main seasons. Bronzing of Thai jackfruit occurred in all growing areas in Tien Giang during both rainy and sunny seasons, causing damage with an average disease prevalence in inquired regions from 5.29% to 10.19% in sunny season, and from 25.43% to 33.05% in rainy season. The disease prevalence was higher in the rainy season. Most farmers showed that bronzing of jackfruit could be identified by external symptoms of the fruit, such as abnormalities in the left stalk, fruit color, shape, and spines, and confirmed by the result of appearance investigation of bronzing on Thai jackfruit during rainy and sunny seasons of the year 2022. Fungicide application and fruit selection at the early stage of fruit development could prevent the disease. According to the disease progress, bronzing of jackfruit appeared from fruit set to harvest and the disease prevalence reached 55.56% in sunny season and 88.89% in rainy season at 20 days after fruit set. 

Lisianthus cultivars are known for their variety of color, size and shape, especially the development of heat tolerant lisianthus for potted ornamentals. The objective of this study was to determine the suitable substrate for good growth and development of some lisianthus cultivars under conditions in Ho Chi Minh City. The two - factor experiment was arranged in a split-plot design with four replications. Factor A (main plot) included three cultivars (Rosita Pink Picotee, Rosita Jade and Rosita Pure White) and factor B (sub-plot) included three substrates (40% coco peat + 60% carbonized rice husk, 40% coco peat + 40% carbonized rice husk + 20% vermicompost và 40% coco peat + 40% carbonized rice husk + 20% cow manure). The results showed that different cultivars of lisianthus significantly affected growth, yield and flower quality. Rosita Jade lisianthus grown on the substrate 40% coco peat + 40% carbonized rice husk + 20% cow manure showed the highest plant height (43.6 cm) and the most number of leaves (43.5 pairs of leaves/plant). Additionally, the application of the substrate 40% coco peat + 40% carbonized rice husk + 20% vermicompost or 40% coco peat + 40% carbonized rice husk + 20% cow manure showed better growth, yield and flower quality of lisianthus compared to 40% coco peat + 60% carbonized rice husk. However, the interaction between cultivar and substrate did not significantly affect lisianthus in terms of number of branches, stem diameter, number of buds, number of flowers, flower diameter and flower longevity.