Effects of low-density lipoprotein extracted from egg yolk on preservation of chilled canine semen (4oC)
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Abstract
Low-density lipoprotein (LDL) derived from hen egg yolk has been considered an important component in the storage of canine semen; however, in Vietnam, there have not been many studies regarding this topic. Therefore, this study aimed to evaluate the effectiveness of LDL in the preservation of canine chilled semen in Vietnam. The assessment of semen samples was based on four criteria: storage time, motility, absolute viability, and integrity of acrosomal membrane at motility rate 0.9; 0.5 and 0.3. The semen samples collected were divided into three equal parts, each was then diluted with three different extenders and stored at 4oC. The extenders were LDL + basic extender (LDL), egg yolk + basic extender (EY), and basic extender (C). The storage time of canine semen was recorded from the beginning of monitoring until the motility decreased to 0.3. The storage time of the three extenders was in the order of EY > LDL > C, with 108 h, 60 h, and 48 h, respectively. The absolute viability of fertilizable sperm (Sa5) in the extender was EY (768), LDL (423) and C (280), which was significant (P < 0.001). The percentage of viable sperm with intact acrosome membrane at motility time A = 0.9 in LDL was 59.31%, higher than that in EY (30.99%). In the storage period from A = 0.9 to A = 0.5, the acrosome loss percentage of both EY and LDL decreased equally (7.30% and 7.23%), but the storage time of EY (84 h) was longer than that of LDL (48 h). In conclusion, the EY gave a longer storage time, higher absolute viability and longer maintain percentage of viable sperms and intact acrosome membrane compared to the LDL. However, within the first hours of storage, the percentage of viable sperm and intact acrosome membrane in the EY was lower than that in the LDL. Therefore, to preserve canine semen for a short time (less than 48 h), the LDL extender should be used for better effectiveness.
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References
Bee, L. E. & Coteerill, O. J. (1979). Ion-exchange chromatography and electrophoresis of egg yolk proteins. Journal of Food Science 44(3), 656-667. https://doi.org/10.1111/j.1365-2621.1979.tb08469.x
Bui, H. T. T. (2010). Preserving ability of some extenders in canine semen (Unpublished bachelor thesis). Nong Lam University, Ho Chi Minh City, Vietnam.
Freshman, J. L. (2001). Clinical management of the subfertile stud dog. Veterinary Clinics of North America: Small Animal Practice 31(2), 259-269. https://doi.org/10.1016/S0195-5616(01)50204-1
Iguer - Ouada, M., & Verstegen, J. P. (2001). Long-term presevervation of chilled canine semen: effect of commercial and laboratory prepared extenders. Theriogenology 55(2), 671-684. https://doi.org/10.1016/S0093-691X(01)00435-6
Johnston, S. D., (1991). Performing a complete canine semen evaluation in a small animal hospital. Veterinary Clinics of North America: Small Animal Practice 21(3), 545-551. https://doi.org/10.1016/S0195-5616(91)50060-7
Kovács, A., & Foote, R. H. (1992). Viability and acrosome staining of bull, boar and rabbit spermatozoa. Biolechnic & Histochemistry 67(3), 119-124. https://doi.org/10.3109/10520299209110020
Milovanov, V. K., (1962). Biology of reproduction and artificial insemination of animals. Moscow, Russia: Selhozizdat.
Moussa, M., Martinet, V., Trimeche, A., Tainturier, D., & Anton, M. (2002). Low density lipoproteins extractedv from hen egg yolk by an easy method: cryoprotective effect on frozen-thawed bull semen. Theriogenology 57(6), 1695-1706. https://doi.org/10.1016/S0093-691X(02)00682-9
Nguyen, A. T., & Nguyen, D. Q., (1997). Artificial insemination of cattle and poultry. Ha Noi, Vietnam: Hannoi Publishing House.
Thai, H. M. T. (2005). Possibility of collecting dog semen and preservation effect of some dog semen extenders (Unpublished master’s thesis). Nong Lam University, Ho Chi Minh City, Vietnam.