Extraction of bioactive components from Paramignya trimera (Oliver) Guillaum stems using an optimized enzyme approach
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Abstract
Xao tam phan (XTP) is a valuable medicinal plant native to Southeast Asia, particularly Vietnam. This plant is rich in bioactive compounds, primarily phenolics and saponins, which offers various therapeutic properties. A recent study optimized the extraction of these bioactive compounds from XTP stems using an enzyme-assisted extraction (EAE). Response surface methodology determined optimal enzyme extraction conditions at 0.8% v/w enzyme concentration, 45°C, and 105 min. Combining ultrasound with enzyme treatment (U-EAE) proved most effective, increased total phenolic content (TPC) and total saponin content (TSC) by 1.23 and 3.27 times, respectively, compared to untreated samples. The U-EAE outperformed other methods (E-UAE, UES-AE, UAE, EAE) in extracting bioactive compounds.
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Dang, B. K., Huynh, T. V, & Le, V. V. M. (2012). Simultaneous treatment of acerola mash by ultrasound and pectinase preparation in acerola juice processing: optimization of the pectinase concentration and pectolytic time by response surface methodology. International Food Research Journal 19(2), 509-513.
Gómez-García, R., Martínez-Ávila, G. C. G., & Aguilar, C. N. (2012). Enzyme-assisted extraction of antioxidative phenolics from grape (Vitis vinifera L.) residues. 3 Biotech 2(4), 297-300. https://doi.org/10.1007/s13205-012-0055-7.
Le, H. T. A., Kim, D. C., Ko, W., Ha, T. M., Nhiem, N. X., Yen, P. H., Tai, B. H., Truong, L. H., Long, V. N., & Gioi, T. (2017). Anti-inflammatory coumarins from Paramignya trimera. Pharmaceutical Biology 55(1), 1195-1201. https://doi.org/10.1080/13880209.2017.1296001.
Lemes, A. C., Egea, M. B., Oliveira Filho, J. G. de, Gautério, G. V., Ribeiro, B. D., & Coelho, M. A. Z. (2022). Biological approaches for extraction of bioactive compounds from agro-industrial by-products: a review. Frontiers in Bioengineering and Biotechnology 9, 802543. https://doi.org/10.3389/fbioe.2021.802543.
Li, F., Mao, Y. D., Wang, Y. F., Raza, A., Qiu, L. P., & Xu, X. Q. (2017). Optimization of ultrasonic-assisted enzymatic extraction conditions for improving total phenolic content, antioxidant and antitumor activities in vitro from Trapa quadrispinosa Roxb. residues. Molecules 22(3), 396. https://doi.org/10.3390/molecules22030396.
Lieu, L. N., & Le, V. V. M. (2010). Application of ultrasound in grape mash treatment in juice processing. Ultrasonics-Sonochemistry 17(1), 273-279. https://doi.org/10.1016/j.ultsonch.2009.05.002.
Miron, T. L., Herrero, M., & Ibáñez, E. (2013). Enrichment of antioxidant compounds from lemon balm (Melissa officinalis) by pressurized liquid extraction and enzyme-assisted extraction. Journal of Chromatography A 1288, 1-9. https://doi.org/10.1016/j.chroma.2013.02.075.
Nag, S., & Sit, N. (2018). Optimization of ultrasound assisted enzymatic extraction of polyphenols from pomegranate peels based on phytochemical content and antioxidant property. Journal of Food Measurement and Characterization 12(3), 1734-1743. https://doi.org/10.1007/s11694-018-9788-2.
Nguyen, T. N. T., Do, A. Q., Trinh, T. T., Le, H. N., Phan, P. H., Phung, V. T., Nguyen, T. T. T., & Nguyen, M. X. H. (2023). Ultrasound probe assisted extraction of Paramignya trimera (Oliver) Guillaum root. IOP Conference Series: Earth and Environmental Science 1155(1), 12021. https://doi.org/10.1088/1755-1315/1155/1/012021.
Nguyen, T. T. N., Trinh, T. T., Le, N. H., Kha, T. C., Phan, H. P., & Nguyen, H. M. X. (2024). Aqueous ultrasound-assisted extraction of phenolics and saponins from xao tam phan plant parts: Optimization and comparison of extraction efficiency. Journal of Technical Education Science 19(03), 77-88. https://doi.org/10.54644/jte.2024.1581.
Nguyen, V. P. T., Le, T. T., & Le, V. V. M. (2013). Application of combined ultrasound and cellulase preparation to guava (Psidium guajava) mash treatment in juice processing: optimization of biocatalytic conditions by response surface methodology. International Food Research Journal 20(1), 377-381.
Nguyen, V. T., Bowyer, M. C., Vuong, Q. Van, Altena, I. A. V., & Scarlett, C. J. (2015). Phytochemicals and antioxidant capacity of Xao tam phan (Paramignya trimera) root as affected by various solvents and extraction methods. Industrial Crops and Products 67(4), 192-200. https://doi.org/10.1016/j.indcrop.2015.01.051.
Puri, M., Sharma, D., & Barrow, C. J. (2012). Enzyme-assisted extraction of bioactives from plants. Trends in Biotechnology 30(1), 37-44. https://doi.org/10.1016/j.tibtech.2011.06.014.
Swer, T. L., Chauhan, K., Paul, P. K., & Mukhim, C. (2016). Evaluation of enzyme treatment conditions on extraction of anthocyanins from Prunus nepalensis L. International Journal of Biological Macromolecules 92, 867-871. https://doi.org/10.1016/j.ijbiomac.2016.07.105.
Tchabo, W., Ma, Y., Engmann, F. N., & Zhang, H. (2015). Ultrasound-assisted enzymatic extraction (UAEE) of phytochemical compounds from mulberry (Morus nigra) must and optimization study using response surface methodology. Industrial Crops and Products 63, 214-225. https://doi.org/10.1016/j.indcrop.2014.09.053.
Wang, J., Sun, B., Liu, Y., & Zhang, H. (2014). Optimisation of ultrasound-assisted enzymatic extraction of arabinoxylan from wheat bran. Food Chemistry 150, 482-488. https://doi.org/10.1016/j.foodchem.2013.10.121.
Zhao, L., Ouyang, D., Cheng, X., Zhou, X., Lin, L., Wang, J., Wu, Q., & Jia, J. (2023). Multi-frequency ultrasound-assisted cellulase extraction of protein from mulberry leaf: Kinetic, thermodynamic, and structural properties. Ultrasonics Sonochemistry 99, 106554. https://doi.org/10.1016/j.ultsonch.2023.106554.