Identification of papaya ringspot virus W type infecting cucurbits in Ho Chi Minh City, Vietnam
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Abstract
Papaya ringspot virus W (PRSV W) is a common aphid transmitted potyvirus, seriously affecting cucurbit production worldwide. This study conducted field surveys to collect PRSV W infecting cucurbit crops in Cu Chi and Hoc Mon districts, two major vegetable-growing areas of Ho Chi Minh City. A total of 48 samples representing sponge gourd, cucumber, bottle gourd, wax gourd, bitter gourd, and pumpkin were collected and tested by indirect ELISA-based serological assay, which detected PRSV in four samples. The indirect ELISA-positive cucurbit samples were then subject to bioassay to identify the type of PRSV, followed by reverse transcription polymerase chain reaction (RT-PCR) confirmation for the PRSV W type. Among all the symptomatic cucurbit samples collected and tested in this study, the highest incidence (27.3%) of PRSV W was recorded on the sponge gourd samples. The infected leaves’extracted total RNA was analyzed by RT-PCR with primer pairs SMK 8701(F)/3’UTR(R), and the PCR product was sequenced. The results revealed that the complete CP genome sequence of isolate PRSV W isolate HocMon (PRSV W-HM) was most closely related (polyprotein nt and aa identities of 93.3 and 95.1%, respectively) to the China isolate of PRSV W (GenBank KX6558741). Briefly, this study provides important insights for the accurate early diagnosis of PRSV W infection and developing effective disease control strategies to limit the spread of this virus isolate.
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References
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