Effects of 60Co gamma-ray irradiation on in vitro shoot regeneration and mutation induction in sweet potato (Ipomoea batatas)
,
,
, &
*
Article Sidebar
Main Article Content
Abstract
Sweet potato (Ipomoea batatas) is a vital crop used for various purposes, including food, feed, and raw materials. However, its production in Vietnam is being adversely affected by climate change, including changes in rainfall patterns, droughts, and saltwater intrusion. To address these challenges, improving crop diversity is essential to enhance yield, nutritional content, and stress tolerance. This study investigated the effects of 60Co gamma-ray irradiation on shoot regeneration from sweet potato shoot tips and nodal segments, followed by the detection of mutations in newly regenerated shoots. The experimental procedures included optimizing the in vitro culture medium and assessing the impact of varying doses of gamma irradiation on survival, mutation formation, and subsequent recovery across generations. The Murashige and Skoog (MS) medium, supplemented with 2 mg/L 6-benzylaminopurine (BAP) and 0.2 mg/L indole-3-butyric acid (IBA), was found to be optimal for shoot regeneration. Gamma irradiation doses ranging from 0 to 90 Gy were applied. The results showed that the lethal dose 50 (LD50) was 59.3 Gy for nodal segments and 80 Gy for shoot tips. The highest regeneration and survival rates were observed at lower radiation doses (30 Gy), while a 50 Gy irradiation dose resulted in a morphological variation frequency of 8.97%, with the most diversified types of morphological variances. Numerous morphological variances including split leaves, chlorophyll loss, light leaf veins, wavy leaves, stems without apical meristems, and abnormal root proliferation, were observed at different rates after irradiation with 30, 50, or 70 Gy. Genetic analysis using Start Codon Targeted (SCoT) markers confirmed genetic differences between the variants and the mother plant, indicating the effectiveness of this approach in enhancing genetic diversity.
Article Details
References
Alam, M. K. (2021). A comprehensive review of sweet potato (Ipomoea batatas [L.] Lam): Revisiting the associated health benefits. Trends in Food Science and Technology 115, 512-529. https://doi.org/10.1016/j.tifs.2021.07.001.
Aslam, F., Naz, S., & Javed, S. (2016). Effect of radiation on morphological characters of different cultivars of Lilium and genetic analysis of mutants through molecular markers. Journal of Animal and Plant Sciences 26(6), 1819-1827.
Aswathy, G. H. N., Vidya, P., & Chokkappan, M. (2016). Analysis of genetic variability in sweet potato accessions using Start Codon Targeted (SCoT) polymorphism. International Journal of Biotechnology and Biochemistry 2(12), 111-121.
Bala, M., & Singh, K. P. (2015). In vitro mutagenesis in rose (Rosa hybrida L.) cv. Raktima for novel traits. Acta Horticulturae 14, 525-531.
Bado, S., Matumelo, A., Rafiri, A. M., Kaoutar, E. K. I., Enoch, S. E., Stephan, N. S., Abdelbagi, M. A., Ghanim, A. M. A., Forster, P. B., & Laimer, M. (2016). In vitro methods for mutation induction in potato (Solanum tuberosum L.). African Journal of Biotechnology 15(39), 2132-2145. https://doi.org/10.5897/AJB2016.15571.
Canti, R. G., & Spear, F. G. (1929). The Effect of gamma irradiation on cell division in tissue culture in vitro.-Part II. Proceedings of the Royal Society B 105(734), 93-98. https://doi.org/10.1098/rspb.1929.0030.
Choi, H. I., Han, S. M., Jo, Y. D., Hong, M. J., Kim, S. H., & Kim, J. B. (2021). Effects of acute and chronic gamma irradiation on the cell biology and physiology of rice plants. Plants 10(3), 439. https://doi.org/10.3390/plants10030439.
Chusreeaeom, K., Sukin, N., & Jompuk, P. (2019). Comparative effect of high energy electron beam and 137Cs gamma ray on survival, growth and chlorophyll content in curcuma hybrid ‘Laddawan’ and determine proper dose for mutations breeding. Emirates Journal of Food and Agriculture 31(5), 321-327. https://doi.org/10.9755/ejfa.2019.v31.i5.1942.
Collard, B. C. Y., & Mackill, D. J. (2009). Start Codon Targeted (SCoT) polymorphism: A simple, novel DNA marker technique for generating gene-targeted markers in plants. Plant Molecular Biology Reporter 27, 86-93. https://doi.org/10.1007/s11105-008-0060-5.
Doyle, J. J., & Doyle, J. L. (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19, 11-15.
Kariuki, K. A., Horemans N, Saenen E., Hees V. M., Verhoeven M., Nauts R., Gompel V. A., Wannijn J., & Cuypers A. (2019). The responses and recovery after gamma irradiation are highly dependent on leaf age at the time of exposure in rice (Oryza sativa L.). Environmental and Experimental Botany 162, 157-167. https://doi.org/10.1016/j.envexpbot.2019.02.020.
Katiyar, P., Pandey, N., & Keshavkant, S. (2022). Gamma radiation: A potential tool for abiotic stress mitigation and management of agroecosystem. Plant Stress 5, 100089. https://doi.org/10.1016/j.stress.2022.100089.
Fadaladeen, L. H., Toma, R. S., Saheen, A. A., & Ahmed, H. B. (2022). A Rapid micropropagation protocol for sweet potato (Ipomoea batatas L.) via tissue culture technique. Diyala Agricultural Sciences Journal 14(1), 31-39. https://doi.org/10.52951/dasj.22140104.
GSOV (General Statistics Office of Vietnam). (2022). The statistics of the yield of annual trees. Retrieved February 4, 2023, from https://www.gso.gov.vn/px-web2.
Hapsari, L., Trimanto, T., Isnaini, Y., & Widiarsih, S. (2021). Morphological characterization and gamma irradiation effect on plant growth of Curcuma heyneana Val & Zijp. AIP Conference Proceedings 2353(1), 030012. https://doi.org/10.1063/5.0052680.
Hasbullah, N. A., Taha, R. M., Saleh, A., & Mahmad, N. (2012). Irradiation effect on in vitro organogenesis, callus growth and plantlet development of Gerbera jamesonii. Horticultura Brasileira 30, 252-257. https://doi.org/10.1590/S0102-05362012000200012.
Hasim, A. A., Shamsiah, A., & Hussein, S. (2021). Induced mutations using gamma ray and multiplication of plantlet through micro cross section culture of banana (Musa acuminata cv. Berangan). IOP Conference Series: Earth and Environmental Science 757(1), 012007. https://doi.org/10.1088/1755-1315/757/1/012007.
Kurucz, E., André, A. Z. S., Fári, M. G., Sipos, M., & Antal, G. (2022). Molecular, phenotypic marker assays, and radiosensitivity tests of gammairradiated Celosia argentea. Acta Horticulturae 54(5), 1049-1065. https://doi.org/10.54910/sabrao2022.54.5.8.
Mohamed, A. E., Osama E., Manal E., Samah A., Kalaji G. S. M. H., Jacek W., & Nabil E. (2021). Impact of gamma irradiation pretreatment on biochemical and molecular responses of potato growing under salt stress. Chemical and Biological Technologies in Agriculture 8, 35. https://doi.org/10.1186/s40538-021-00233-8.
Murashige, T., & Skoog F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarium 15(3), 473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x.
Normasari, R., Arumingtyas, E. L., Retnowati, R., & Widoretno, W. (2023). In vitro mutagenesis on patchouli (Pogostemon cablin Benth.) with gamma-ray irradiation on leaf explants. Biodiversitas Journal of Biological Diversity 24(12), 6407-6414. https://doi.org/10.13057/biodiv/d241201.
Oladosu, Y., Rafii, M. Y., Abdullah, N., Hussin, G., Ramli, A., Rahim, H. A., Miah, G., & Usman, M. (2015). Principle and application of plant mutagenesis in crop improvement: a review. Biotechnology and Biotechnological Equipment 30(1), 1-16. https://doi.org/10.1080/13102818.2015.1087333.
Peng, Y., Hayat, F., Li, J., Xiao, J. Q., Tu, P. F., Chen, J. Z., Li, C. Q., Song, W. P., & Xuan, W. S. (2023). Genetic diversity analysis of 20 Abiu germplasm based on SCoT molecular markers. Applied Ecology and Environmental Research 21(5), 4295-4311. https://doi.org/10.15666/aeer/2105_42954311.
Puripunyavanich, V., Maikaeo, L., Limtiyayothin, M., & Orpong, P. (2022). New frontier of plant breeding using gamma irradiation and biotechnology. In Kumar, B., & Debut, A. (Eds.). Green chemistry - New perspectives. London, UK: IntechOpen. https://doi.org/10.5772/intechopen.104667.
Qi, W., Zhang, L., Feng, W., Xu, H., Wang, L., & Jiao, Z. (2015). ROS and ABA signaling are involved in the growth stimulation induced by low-dose gamma irradiation in Arabidopsis seedling. Applied Biochemistry and Biotechnology 175, 1490-1506. https://doi.org/10.1007/s12010-014-1372-6.
Rajesh, M. K., Sabana, A. A., Rachana, K. E., Rahman, S., Jerard, B. A., & Karun, A. (2015). Genetic relationship and diversity among coconut (Cocos nucifera L.) accessions revealed through SCoT analysis. 3 Biotech 5, 999-1006. https://doi.org/10.1007/s13205-015-0304-7.
Ramchander, S., Andrew-Peter-Leon, M. T., Khan, Y. J., Souframanien, J., & Pillai, M. A. (2024). Molecular and physiological characterization of early semi-dwarf mutants of rice and localization of SNP variants in Sd1 locus generated through gamma radiation. International Journal of Radiation Biology, 100(4), 650-662. https://doi.org/10.1080/09553002.2024.2304827.
Roslim, D. I., Herman., & Fiatin, I. (2015). Lethal dose 50 (LD50) of mungbean (Vigna radiata L. wilczek) cultivar kampar. SABRAO Journal of Breeding and Genetics 47(4), 510-516.
Shaozhen, H. Y., & Wang, Y. (2009). In vitro selection and identification of sweet potato (Ipomoea batatas (L.) Lam.) plants tolerant to NaCl. Plant Cell Tissue Organ Culture 96, 69-74. https://doi.org/10.1007/s11240-008-9461-2.
Shin, J. M., Kim, B. K., Seo, S. G., Jeon, S. B., Kim, J. S., Jun, B. K., Kang, S. Y., Lee, J. S., Chung, M. N., & Kim, S. H. (2011). Mutation breeding of sweet potato by gamma-ray radiation. African Journal of Agricultural Research 6(6), 1447-1454. https://doi.org/10.5897/AJAR10.936.
Tu, L. T. N., Hop, T. D., Phuong, T. T. A., Linh, N. N. A., & Tam, N. T. A. (2019). Study on propagating Japanese sweet potato by cell tissue culture method. Journal of Vietnam Agricultural Science and Technology 7(104), 57-64.
Wang, Y., Wang F., Zhai H., & Liu, Q. (2007). Production of a useful mutant by chronic irradiation in sweet potato. Scientia Horticulturae 111(2), 173-178. https://doi.org/10.1016/j.scienta.2006.10.007.
Wahyudi, D., Belina, V., Suyono., Resmisari, R., & Fitriyah. (2024). Effects of gamma irradiation on morphological changes in Porang (Amorphophallus muelleri Blume). IOP Conference Series: Earth and Environmental Science 1312, 012040. https://doi.org/10.1088/1755-1315/1312/1/012040.