Effect of NaOCl, growth media, and plant growth regulators on in vitro propagation of disease-free KM140 cassava cultivar (Manihot esculenta Crantz)
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Abstract
Cassava mosaic disease (CMD) is one of the most dangerous diseases that has caused heavy losses in yield and starch content on cassava. In vitro propagation using disease-free cassava stakes was an optimal method to produce healthy seedlings. In this study, sodium hypochlorite (NaOCl), growth media (MS, ½ MS and Knudson C) and growth regulators (BA: Benzyl adenine, NAA: Naphthalene acetic acid, GA: Gibberellin) were used to determine an appropriate time for sample sterilization and the suitable concentration for shoot multiplication and root induction of KM140 cassava cultivar. The results showed that sample sterilization at 8% NaOCl concentration in 5 min gave the highest survival rate (71.7%) at 14 d of culture. Cassava explants cultured on MS medium supplemented with 1 mg/L BA gained the highest number of shoots (2.3 shoots), shoot height (10.3 mm) and the number of leaves (4.2 leaves/shoot) at 50 d of culture. The MS medium supplemented with 0.07 mg/L NAA and 0.03 mg/L GA was applicable for the rooting stage of KM140 cassava cultivar (19.8 roots/plantlet at 60 d of culture). The results of the study were fundamental to in vitro propagation process of disease-free KM140 cassava multiplication.
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References
Abdoulaye, F. A. Y. E., Sagna, M., Kane, P. D., & Djibril, S. A. N. E. (2015). Effects of different hormones on organogenesis in vitro of some varieties of cassava (Manihot esculenta Crantz) grown in Senegal. African Journal of Plant Science 9(8), 305-312. https://doi.org/10.5897/AJPS2014.1243.
Alla, N. A. A., Ragab, M. E., El-Miniawy, S. E. M., & Taha, H. S. (2013). In vitro studies on cassava plant micropropagation of cassava (Manihot esculenta Crantz). Journal of Applied Sciences Research 9(1), 811-820.
Escobar, R. H., Restrepo, J., Tohme, J., & Roca, W. M. (2013). Use of tissue culture in cassava for rural households in Colombia. In: Ruane, J., Dargie, J. D., Mba, C., Boettcher, P., Makkar, H. P. S., Bartley, D. M., & Sonnino, A. (Eds). Biotechnologies at work for smallholders: Case studies from developing countries in crops, livestock and fish (1st ed., 56-62). Rome, Italy: Food and Agriculture Organization of the United Nations.
Fletcher, E. K., Amoako, T. N., & Twumasi, P. (2011). Effect of 2, 4-D, explants type and cultivar on the callogenesis expression of cassava (Manihot esculenta Crantz) in Ghana. African Journal of Biotechnology 10(46), 9396-9401. https://doi.org/10.5897/AJB10.2115.
Hamill, S. D. (2014). Processes, costs and traits of plants produced in tissue culture must be considered to develop effective crop production systems. In Lambardi, M., Hamill, S. and Drew, R. (Eds.), XXIX International Horticultural Congress on Horticulture: Sustaining Lives, Livelihoods and Landscapes (IHC2014): 1113 (85-92). Brisbane, Australia: ISHS. https://doi.org/10.17660/ActaHortic.2016.1113.12.
Hy, N. H., Reinhardt, H., Nhan, P. T., & Buu, B. C. (2020). Cassava science. Ha Noi, Vietnam: Agricultural Publishing House.
Mapayi, E. F., Ojo, D. K., Oduwaye, O. A., & Porbeni, J. B. O. (2013). Optimization of in-vitro propagation of cassava (Manihot esculenta Crantz) genotypes. Journal of Agricultural Science 5(3), 261. https://doi.org/10.5539/jas.v5n3p261.
Maruthi, M. N., Whitfield, E. C., Otti, G., Tumwegamire, S., Kanju, E., Legg, J. P., Mkamilo, G., Kawuki, R., Benesi, I., Zacarias, A., & Munga, T. (2019). A method for generating virus-free cassava plants to combat viral disease epidemics in Africa. Physiological and Molecular Plant Pathology 105, 77-87. https://doi.org/10.1016/j.pmpp.2018.09.002.
Minh T. V. (2005). Plant cell technology (1st ed.). Ha Noi, Vietnam: Vietnam Academy of Science and Technology.
Muimba-Kankolongo, A., Chalwe, A., Sisupo, P., & Kang, M. S. (1997). Distribution, prevalence and outlook for
control of cassava mosaic disease in Zambia. Roots 4(1), 2-7.
Noh, S. A., Lee, H. S., Huh, E. J., Huh, G. H., Paek, K. H., Shin, J. S., & Bae, J. M. (2010). SRD1 is involved in the auxin-mediated initial thickening growth of storage root by enhancing proliferation of metaxylem and cambium cells in sweet potato (Ipomoea batatas). Journal of Experimental Botany 61(5), 1337-1349. https://doi.org/10.1093/jxb/erp399.
Sessou, A. F., Kahia, J. W., Houngue, J. A., Ateka, E. M., Dadjo, C., & Ahanhanzo, C. (2020). In vitro propagation of three mosaic disease resistant cassava cultivars. BMC Biotechnology 20(1), 1-13. https://doi.org/10.1186/s12896-020-00645-8.
Thresh, J. M., Otim-Nape, G. W., Legg, J. P., & Fargette, D. (1997). Africa cassava mosaic disease: What is the magnitude of the problem. In Thro, R. M. and Akoroda, M. P.(Eds), Proceedings of The Cassava Biotechnology Network Third International Scientific Meeting. Ibadan, Nigeria: International Society for Tropical Root Crops - Africa Branch (ISTRC-AB).
Uke, A., Hoat, T. X., Quan, M. V., Liem, N. V., Ugaki, M., & Natsuaki, K. T. (2018). First report of Sri Lankan cassava mosaic virus infecting cassava in Vietnam. Plant Disease 102(12), 2669. https://doi.org/10.1094/PDIS-05-18-0805-PDN.