Evaluation of the control potential to root - knot nematode Meloidogyne incognita of Purpureocillium lilacinum 11BB strain
,
,
,
, &
*
Article Sidebar
Main Article Content
Abstract
Parasitic fungi are considered effective biological agents to control plant -parasitic nematodes. Based on morphology and ITS sequence, seven Purpureocillium lilacinum strains were isolated from 144 soil samples collected from Chau Duc district, Ba Ria - Vung Tau province, and Dinh Quan district, Dong Nai province. After 96 h of culture in medium supplemented with casein, chitin, and tween 20, seven strains showed protease, chitinase, and lipase activity. In laboratory conditions, both 11BB and 11SN strains parasitzed 31 – 34% eggs and 58 – 62% eggmass, respectively. In greenhouse conditions, the 11BB strain decreased 79.0 - 80.3% of juveniles (J2s) and 77.4 - 79.7% of egg numbers on tomato plants as compared with the control. Results showed that the 11BB fungal strain could be used in control of root - knot nematodes.
Article Details
References
Atkins, S. D., Clark, I. M., Pande, S., Hirsch, P. R., & Kerry, B. R. (2005). The use of real - time PCR and species - specific primers for the identification and monitoring of Paecilomyces lilacinus. FEMS Microbiology Ecology 51(2), 257-264. https://doi.org/10.1016/j.femsec.2004.09.002.
Gopinath, S. C., Anbu, P., Lakshmipriya, T., & Hilda, A. (2013). Strategies to characterize fungal lipases for applications in medicine and dairy industry. BioMed research international, 2013, ID154549. https://doi.org/10.1155/2013/154549.
Izumitsu, K., Hatoh, K., Sumita, T., Kitade, Y., Morita, A., Gafur, A., Ohta, A., Kawai, M., Yamanaka, T., Neda, H., Ota, Y., & Tanaka, C. (2012). Rapid and simple preparation of mushroom DNA directly from colonies and fruiting bodies for PCR. Mycoscience 53(5), 396-401. https://doi.org/10.1007/S10267-012- 0182-3.
Khan, A., Williams, K. L., & Nevalainen, H. K. (2006). Infection of plant - parasitic nematodes by Paecilomyces lilacinus and Monacrosporium lysipagum. Biological Control 51(5), 659-678. https://doi.org/10.1007/s10526-005-4242-x.
Khan, A., Williams, K. L., & Nevalainen, H. K. (2004). Effects of Paecilomyces lilacinus protease and chitinase on the eggshell structures and hatching of Meloidogyne javanica juveniles. Biological Control 31(3), 346-352. https://doi.org/10.1016/j.biocontrol.2004.07.011.
Le, C. T. M., Le, N. T. T., Vu, D. T., Nguyen, D. T. T., Pham, H. N. Đ., & Pham, N. H. (2016). Selection of indigenous strains of Purpureocillium lilacinum able to parasitize root - knot nematode meloidogyne spp. Journal of Plant Protection 2, 24-29.
Le, P. H. (2009). Isolation and selection of media for three insect parasitic fungi Metarhizium anisopliae (Metsch.) Sorok, Beauveria bassiana (Bals.) Vuill and P. lilacinum on leaf vegetables in the Mekong Delta (Research report). An Giang University, An Giang, Vietnam.
Luangsa - Ard, J., Houbraken, J., van Doorn, T., Hong, S. B., Borman, A. M., Hywel - Jones, N. L., & Samson, R. A. (2011). Purpureocillium, a new genus for the medically important Paecilomyces lilacinus. FEMS microbiology letters 321(2), 141-149. https://doi.org/10.1111/j.1574-6968.2011.02322.x.
Moosavi, M. R., & Zare, R. (2012). Fungi as biological control agents of plant - parasitic nematodes. In Mérillon, J. M., & Ramawat, K. G. (Eds.). Plant defence: Biological Control (2nd ed., 67-107). Dordrecht, Netherlands: Springer. https://doi.org/10.1007/978-94-007-1933-0_4.
Morton, O., Hirsch, P., & Kerry, B. (2004). Infection of plant - parasitic nematodes by nematophagous fungi–a review of the application of molecular biology to understand infection processes and to improve biological control. Nematology 6(2), 161-170. https://doi.org/10.1163/1568541041218004.
Mustafa, U., & Kaur, G. (2010). Studies on extracellular enzyme production in Beauveria bassiana isolates. International Journal of Biotechnology and Biochemistry 6(5), 701-714.
Ngo, X. T. (2000). Study on biological characteristics and the ability to prevent root - knot nematode Meloidogyne incognita (Kofoid et White, 1919/Chitwood) on some crops in Hanoi and surrounding areas (Unpublished master’s thesis). Ha Noi University of Agriculture I, Ha Noi, Vietnam.
Nguyen, M. T. H., Dao, H. T. T., Nguyen, T. Đ., Nguyen, Q. T., Dao, H. H., Ho, H., Tran, N. K., Nguyen, H. T., Vo, N. T., & Pham, T. V. (2020a). Selection to determining the consortium of microorganisms antagonistic to coffee pathogen fungi and parasitic nematode. The Journal of Agriculture and Rural Development 397, 3-10.
Nguyen, D. T., Nguyen, T. H., Le, L. T. M., & Trinh, P. Q. (2020b). Effects of Paecilomyces sp. to Meloidogyne incognita and Pratylenchus penetrans in laboratory conditions. In The 19th National Conference of Plant Protection (190-200). Ha Noi, Vietnam: Agricultural Publishing House.
Nguyen, P. V. (2018). Construction of an artificial microRNA expression vector for silencing a gene of Meloidogyne incognita. The Journal of Agriculture and Development 17(2), 48-54.
Nicol, J. M., Turner, S. J., Coyne, D. L., den Nijs, L. J. M. F., Hockland, S., & Maafi, Z. T. (2011). Current nematode threats to world agriculture. In Jones, L., Gheysen, G., & Fenoll, C. (Eds.). Genomics and molecular genetics of plant - nematode interactions (21 - 43). Dordrecht, Netherlands: Springer. https://doi.org/10.1007/978-94-007-0434-3_2.
Pau, C. G., Leong, C. T. S., Wong, S. K., Eng, L., Jiwan, M., Kundat, F. R., Zakry Fitri, A. A., Osumanu Haruna, A., & Majid, N. M. (2012). Isolation of indigenous strains of Paecilomyces lilacinus with antagonistic activity against Meloidogyne incognita. International Journal of Agriculture and Biology 14(2), 197-203.
Samson, R. A. (1974). Paecilomyces and some allied hyphomycetes. Utrecht, Netherlands: Centraalbureau voor Schimmelcultures.
Siddiqui, Z. A., & Mahmood, I. (1996). Biological control of plant parasitic nematodes by fungi: a review. Bioresource Technology 58(3), 229-239. Tran, T. D. (2009). Beauveriolide I, a cyclopeptide from the insect parasitic fungus (Paecilomyces lilacinus (Thom) Samson) in Nghe An (Unpublished master’s thesis). Vinh University, Nghe An, Vietnam.
Trivedi, P. C. (2012). Plant parasitic nematodes and their management by bioagents. In Proceedings of the 99th Indian Science Congress. Bhubaneswar, India: KIIT University.
Zhang, F., & Schmitt, D. P. (1994). Host status of 32 plant species to Meloidogyne konaensis. Journal of Nematology 26(4S), 744-748.