Isolation, immobilization of salt-tolerant and inorganic phosphate solubilizing bacteria for producing controlled-release inorganic fertilizer in combination with microorganisms
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Abstract
Salt tolerance is one of the characteristics to ensure the viability of microorganisms when combined with chemical fertilizers. This research aimed to isolate bacteria that had both phosphate-solubilizing and salt-tolerant abilities for production of controlled-release inorganic fertilizer-incorporated microorganisms. Of twenty-five phosphate solubilizing bacteria strains isolated from soil samples collected from Ho Chi Minh City, Dong Nai, and Long An provinces on Pikovskaya medium (PVK), there were three strains of bacteria that had both phosphate solubilizing activity and salt tolerance. The analysis results on PVK medium supplemented with 3% and 4% NaCl showed that only PSM54 strain had phosphorusdegrading ring. Species identification based on 16S-rRNA sequence showed that PSM54 was 99.9% similar to Bacillus velezensis. The controlled-release fertilizer was made by coating biodegradable polymers incorporated with PSM54 bacteria that met standards for slow dissolution according to The Association of American Plant Food Control Officials. After 60 days of being immobilized in the membrane of controlled release, the PSM54 bacteria remained at 88.3% compared to the initial density.
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References
AAPFCO (Association of American Plant Food Control Officials). (1997). Official publication - Association of American plant food control officials issue 50. Indiana, USA: AAPFCO Inc.
Azeem, B., KuShaari, K., Man, Z. B., Basit, A., & Trinh, T. H. (2014). Review on materials and methods to produce controlled release coated urea fertilizer. Journal of controlled Release 181, 11-21, from https://doi.org/10.1016/j.jconrel.2014.02.020.
Bhattacharyya, P. N., & Jha, D. K. (2012). Plant growthpromoting rhizobacteria (PGPR): emergence in agriculture. World Journal of Microbiology and Biotechnology 28(4), 1327-1350 https://doi.org/10.1007/s11274-011-0979-9.
Geisseler, D., & Scow, K. M. (2014). Long-term effects of mineral fertilizers on soil microorganisms - A review. Soil Biology and Biochemistry 75, 54-63. https://doi.org/10.1016/j.soilbio.2014.03.023.
Gyaneshwar, P., Kumar, G. N., Parekh, L. J., & Poole, P. S. (2002). Role of soil microorganisms in improving P nutrition of plants. Plant and Soil 245(1), 83-93. https://doi.org/10.1023/A:1020663916259.
Hoang, M. T., Vu, Q. S., & Nguyen, K. T. (2004). A textbook of plant physiology. Ho Chi Minh City, Vietnam: Pedagogical Publishing House.
Hwangbo, K., Um, Y., Kim, K. Y., Madhaiyan, M., Sa, T. M., & Lee, Y. (2016). Complete genome sequence of Bacillus velezensis CBMB205, a phosphatesolubilizing bacterium isolated from the rhizoplane of rice in the Republic of Korea. ASM Journal - Genome Announcements 4(4), 16. https://doi.org/10.1128/genomeA.00654-16.
Joly, P., Calteau, A., Wauquier, A., Dumas, R., Beuvin, M., Vallenet, D., Crovadore, J., Cochard, B., Lefort, F., & Berthon, J. Y. (2021). From strain characterization to field authorization: Highlights on Bacillus velezensis strain B25 beneficial properties for plants and its activities on phytopathogenic fungi. Microorganisms 9(9), 1924. https://doi.org/10.3390/microorganisms9091924.
Kourkoutas, Y., Bekatorou, A., Banat, I. M., Marchant, R., & Koutinas, A. A. (2004). Immobilization technologies and support materials suitable in alcohol beverages production: a review. Food Microbiology 21(4), 377-397. https://doi.org/10.1016/j.fm.2003.10.005.
Madhaiyan, M., Poonguzhali, S., Kwon, S. W., & Sa, T. M. (2010). Bacillus methylotrophicus sp. nov., a methanol-utilizing, plant-growth-promoting bacterium isolated from rice rhizosphere soil. International Journal of Systematic and Evolutionary Microbiology 60(10), 2490-2495. https://doi.org/10.1099/ijs.0.015487-0.
Mayer, H. (2010). Nutrient release patterns of controlled release fertilizers used in the ornamental horticulture industry of south Florida (Unpublished doctoral dissertation). The University of Florida, The State of Florida, USA.
Medina, L. C., Obreza, T. A., Sartain, J. B., & Rouse, R. E. (2008). Nitrogen release patterns of a mixed controlled-release fertilizer and its components. HortTechnology 18(3), 475-480. https://doi.org/10.21273/HORTTECH.18.3.475.
Sartain, J. B., Hall, W. L., Littell, R. C., & Hopwood, E. W. (2004). New tools for the analysis and characterization of slow-release fertilizers. In Hall, W. L., & Robarge, W. P. (Eds.). Environmental Impact of Fertilizer on Soil and Water (180-195). Oxford, UK: Oxford University Press. https://doi.org/10.1021/bk-2004-0872.ch013.
Saxena, A. K., Kumar, M., Chakdar, H., Anuroopa, N., & Bagyaraj, D. J. (2020). Bacillus species in soil as a natural resource for plant health and nutrition. Journal of Applied Microbiology 128(6), 1583-1594. https://doi.org/10.1111/jam.14506.
Sharma, S. B., Sayyed, R. Z., Trivedi, M. H., & Gobi, T. A. (2013). Phosphate solubilizing microbes: sustainable approach for managing phosphorus deficiency in agricultural soils. SpringerPlus 2(1), 1-14. https://doi.org/10.1186/2193-1801-2-587.
Sharpley, A. N. (1995). Soil phosphorus dynamics: agronomic and environmental impacts. Ecological Engineering 5(2-3), 261-279. https://doi.org/10.1016/0925-8574(95)00027-5.
Shaviv, A. (2001). Advances in controlled-release fertilizers. Advances in Agronomy 71, 1-49. https://doi.org/10.1016/S0065-2113(01)71011-5.
Syers, K., Bekunda, M., Cordell, D., Corman, J., Johnston, J., Rosemarin, A., Salcedo, I., & Lougheed, T. (2011). Phosphorus and food production. UNEP Year Book, 34-45. https://fsc.uni-hohenheim.de/fileadmin/einrichtungen/fsc/Intranet/Intranet_
MOSA/MOSA_Updated/5_UNEP_2011.pdf.
Trenkel, M. E. (2010). Slow-and controlled-release and stabilized fertilizers: An option for enhancing nutrient use efficiency in agriculture. Paris, France: Published by the International Fertilizer Industry Association (IFA). http://repo.upertis.ac.id/1628/1/2010_Trenkel_slow%20release%20book.pdf.