Tuan V. Vo * , Truc T. T. Nguyen , Binh T. T. Vo , & Duyen T. H. Nguyen

* Correspondence: Vo Van Tuan (email: vovantuan@hcmuaf.edu.vn)

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Effects of water pH on blood physiological parameters and color change of Asian bumblebee catfish (Pseudomystus siamensis) (4 - 6 g/fish) were carried out in laboratory condition. The experiment was set up in 8 weeks at different pH water levels (pH = 3, 4, 5, 6, 7, 8, 9, 10, 11). The results have shown that the cumulative mortality ratio of Asian bumblebee catfish at the end of 24 h challenge was 100% at pH = 11, 70.83% at pH = 10, and 62.5% at pH = 3. No mortality of fish was observed at pH = 4, 5, 6, 7, 8, 9 after 24 h of the challenge. The lowest and highest pH threshold that killed 50% of fish after 24 h of the challenge were 3.04 and 9.95, respectively. After 24 h of the challenge, total number of red and white blood cells of fish increased at pH = 3, 9, 10, and get the highest level at pH = 3 (1.87 × 106 cells/mm3và 1.59 × 105 cells/mm3, respectively. At the end of the challenge, highest number of red and white blood cells were observed at pH = 8 (2 ± 0.23 × 106 cells/mm3 và 1.27 ± 0.26 × 105 cells/mm3, respectively). Fish were in bright and beautiful color when cultured in high pH water levels.

Keywords: Blood cells, Color, pH threshold, Pseudomystus siamensis

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