Separation of active constituents in some medicinal plants by thin layer chromatography
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Abstract
Separation and identification of the active pharmacological compounds from some medicinal plants (Citrus aurantifolia, Rhodomyrtus tomentosa, Camellia sinensis) by Thin Layer Chromatography (TLC) showed the yields of crude extracts ob-tained from Citrus aurantifolia, Rhodomyrtus tomentosa and Camellia sinensis leaves at 1.5%, 5.62% and 10.4% respectively. Toluene: ethyl acetate (93:7) (v:v) solvent was suitable for the separation of active compounds in crude extract of Citrus auran-tifolia, Rhodomyrtus tomentosa leaves while chloroform: ethy-lacetate: formic acid (5:4:1) (v:v:v) solvent was suitable for the extraction of active compounds in crude extract from Camelliasinensis leaves. Furthermore, the results showed that the num-ber of compounds in extract from Citrus aurantifolia were like lyaffected by the vacuum evaporator effects. The TLC fingerprints of all three medicinal plants had the same visual ability when the fingerprint detected by UV (λ= 254 nm) and the reagent containing 0.1 g vanillin in 28 mL of methanol: 1 mL of sulfuric acid. In brief, the three active compounds including citral (Cit-rus aurantifolia), rhdomyrtone (Rhodomyrtus tomentosa) and catechin hydrate (Camellia sinensis) contained in the extract of 3 medicinal herbs had the limited detection (LOD) at 195 ng/spot, 321.5 ng/spot and 625 ng/spot, respectively.
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References
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