Innate immune responses of whiteleg shrimp (Penaeus vannamei) experimentally infected with acute hepatopancreas necrosis disease-causing Vibbrio parahaemolyticus
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Abstract
The innate immune responses of the whiteleg shrimps (Penaeus vannamei) experimentally challenged with V. parahaemolyticus by immersion were investigated for a period of 120 h. The results showed that the lethal dose 50% (LD50) of shrimps (2 - 3 g) challenged with V. parahaemolyticus was 4.7×106 CFU/mL. No significant differences in immune parameters were observed between the control and challenged group right after challenge (0 hpi). However, the total haemocyte count, phenoloxidase activity and respiratory burstsactivity were decreased in the challenged shrimps after 24 and 48 hpi and significantly different from those in the control shrimps (P < 0,05). At 72, 96 and 120 hpi, there were no significant differences in the total haemocyte count, phenoloxidase activity and respiratory burst activity between two treatments. The observations of this study showed that the innate immune responses of the white-leg shrimp were decreased due to the infection by V. parahaemolyticus.
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References
Bachere, E., Gueguen, Y., Gonzalez, M., De Lorgeril, J., Garnier, J., & Romestand, B. (2004). Insights into the anti-microbial defense of marine invertebrates: the penaeid shrimps and the oyster Crassostrea gigas. Immunological Reviews 198(1), 149-168. https://doi.org/10.1111/j.0105-2896.2004.00115.x
Dang, O. T. H., Le, T. H., & Nguyen, P. T. (2012). Optimization and application of protocols for immune response analysis in Macrobrachium rosenbergii. Can Tho University Journal of Science 21b, 10-18.
Dantas-Lima, J. J., Vo, T. V., Corteel, M., Grauwet, K., An, N. T. T., Sorgeloos, P., & Nauwynck, H. J. (2013). Separation of Penaeus vannamei haemocyte subpopulations by iodixanol density gradient centrifugation. Aquaculture 408-409, 128-135. https://doi.org/10.1016/j.aquaculture.2013.04.031
Hansen (2000). Use of a hemacytometer. Laboratory procedures, Department of Animal Sciences, University of Florida, Florida, USA.
Hernández-López, J., Gollas-Galván, T., & VargasAlbores, F. (1996). Activation of the prophenoloxidase system of the brown shrimp (Penaeus californiensis, Holmes). Comparative Biochemical Physiology 113C (1), 61-66. https://doi.org/10.1016/0742-8413(95)02033-0
Hrubec, C. T., Cardinale, J. L., & Smith, S. A. (2000). Hematology and plasma chemistry reference intervals for cultured tilapia (Oreochromis hydrid). Veterinary Clinical Pathology 29(1), 7-12. https://doi.org/10.1111/j.1939-165X.2000.tb00389.x
Hsieh, S. L., Ruan, Y. H., Li, Y. C., Hsieh, P. S., Hu, C. H., & Kuo, C. M. (2008). Immune and physiological responses in Pacific white shrimp (Penaeus vannamei) to Vibrio alginolyticus. Aquaculture 275(1-4), 335-341. https://doi.org/10.1016/j.aquaculture.2007.12.019
Hsu, S. W., & Chen, J. C. (2007). The immune response of white shrimp Penaeus vannamei and its susceptibility to Vibrio alginolyticus under sulfide stress. Aquaculture 271(1-4), 61-69. https://doi.org/10.1016/j.aquaculture.2007.05.028
Johansson, M. W., Keyser, P., Sritunyalucksana, K., & S¨oderh¨all, K. (2000). Crustacean haemocytes and haematopoiesis. Aquaculture 191(1-3), 45-52. https://doi.org/10.1016/S0044-8486(00)00418-X
Jose, S., Mohandas, A., Philip, R., & Bright Singh, I. S. (2010). Primary hemocyte culture of Penaeus monodon as an in vitro model for white spot syndrome virus titration, viral and immune related gene expression and cytotoxicity assays. Journal of Invertebrate Pathology 105(3), 312-321. https://doi.org/10.1016/j.jip.2010.08.006
Le Moullac, G., & Haffner, P. (2000). Environmental factors affecting immune responses in Crustacea. Aquaculture 191(1-3), 121-131. https://doi.org/10.1016/S0044-8486(00)00422-1
Lee, C. T., Chen, I. T., Yang, Y. T., Ko, T. P., Huang, Y. T., Huang, J. Y., Huang, M. F., Lin, S. J., Chen, C. Y., Lin, S. S., Lightner, D. V., Wang, H. C., Wang, A. H., Wang, H. C., Hor, L. I., & Lo, C. F. (2015). The opportunistic marine pathogen Vibrio parahaemolyticus becomes virulent by acquiring a plasmid that expresses a deadly toxin. Proceedings of National Academy of Sciences U.S.A 112(34), 10798-10803. https://doi.org/10.1073/pnas.1503129112
Lightner, D. (2011). Virus diseases of farmed shrimp in the Western Hemisphere (the Americas): A review. Journal of Invertebrate Pathology 106(1), 110-130. https://doi.org/10.1016/j.jip.2010.09.012
Liu, C. H., Yeh, S. T., Cheng, S. Y., & Chen, J. C. (2004). The immune response of white shrimp Litopenaeus vannamei and its susceptibility to Vibrio infection in relation with the moult cycle. Fish & Shellfish Immunology 16(2), 151-161. https://doi.org/10.1016/S1050-4648(03)00058-5
Lopez-Leon, P., Luna-Gonzalez, A., Escamilla-Montes, R., Flores-Miranda, M. C., Fierro-Coronado, J. A., Alvarez-Ruiz, P., & Diarte-Plata, G. (2016). Isolation and characterization of infectious Vibrio parahaemolyticus, the causative agent of AHPND, from the whiteleg shrimp (Litopenaeus vannamei). Latin American Journal of Aquatic Research 44(3), 470-479. https://doi.org/10.3856/vol44-issue3-fulltext-5
Matozzo, V., & Marin, M. G. (2010). The role of haemocytes from the crab Carcinus aestuarii (Crustacea, Decapoda) in immune responses: A first survey. Fish & Shellfish Immunology 28(4), 534-541. https://doi.org/10.1016/j.fsi.2009.12.003
Menz, A., & Blake, B. F. (1980). Experiments on the growth of Penaeus vannamei Boone. Journal of Experimental Marine Biology and Ecology 48(2), 99-111. https://doi.org/10.1016/0022-0981(80)90010-6
Reed, L. J., & Muench, H. (1938). A simple method of estimating fifty per cent endpoints. American Journal of Hygiene 27(3), 493-497. https://doi.org/10.1093/oxfordjournals.aje.a118408
Perazzolo, L. M., & Barracco, M. A. (1997). The prophenoloxidase activating system of the shrimp Penaeus paulensis and associated factors. Developmental Comparative Immunology 21(5), 385-395.
Robertson, P. A. W., Calderon, J., Carrera, L., Stark, J. R., Zherdmant, M., & Austin, B. (1998). Experimental Vibrio harveyi infections in Penaeus vannamei larvae. Diseases of Aquatic Organism 32(2), 151-155. https://doi.org/10.3354/dao032151
S¨oderh¨all, K., & Smith, V. J. (1983). Separation of the haemocyte populations of Carcinus maenas and other marine decapods, and prophenoloxidase distribution. Developmental & Comparative Immunology 7(2), 229-239. https://doi.org/10.1016/0145-305X(83)90004-6
Song, Y. L., & Hsieh Y. T. (1994). Immunostimulation of tiger shrimp (Penaeus monodon) hemocytes for generation of micribicidal substances: Analysis of reactive oxygen species. Developmental and Comparative Immunology 18(3), 201-209. https://doi.org/10.1016/0145-305X(94)90012-4
Song, Y. L., Yu, C. I., Lien, T. W., Huang, C. C., & Lin, M. N. (2003). Haemolymph parameters of Pacific white shrimp (Litopenaeus vannamei) infected with Taura syndrome virus. Fish & Shellfish Immunology 14(4), 317-331. https://doi.org/10.1006/fsim.2002.0440
Sritunyalucksana, K., & S¨oderh¨all, K. (2000). The proPO and clotting system in crustaceans. Aquaculture 191, 53-69. https://doi.org/10.1016/S0044-8486(00)00411-7
Thakur, A. B., Vaidya, R. B., & Suryawanshi S. A. (2003). Pathogenicity and antibiotic susceptibility of Vibrio species isolated from moribund shrimps. Indian Journal of Marine Sciences 32(1), 71-75.
Tran, L. H., Nunan, L., Redman, R.M., Mohney, L. L., Pantoja, C. R., Fitzsimmons, K., & Lightner, D. V. (2013). Determination of the infectious nature of the agent of acute hepatopancreatic necrosis syndrome affecting penaeid shrimp. Diseases of Aquatic Organism 105(1), 45-55. https://doi.org/10.3354/dao02621
Tseng, I. T., & Chen, J. C. (2004). The immune response of white shrimp Litopenaeus vannamei and its susceptibility to Vibrio alginolyticus under nitrite stress. Fish & Shellfish Immunology 17(4), 325-333. https://doi.org/10.1016/j.fsi.2004.04.010
Van de Braak, C., Faber, R., & Boon, J. H. (1996). Cellular and humoral characteristics of Penaeus monodon (Fabricius, 1798) haemolymph. Comparative Haematology International 6(4), 194-203. https://doi.org/10.1007/BF00378110