Nghiên cứu quy trình nhân giống in vitro cây Giảo cổ lam (Gynostemma pentaphyllium Thunb.)

Abstract

Giao co lam (Gynostemma pentaphyllium Thunb.) is a traditional medicine plant and endangered species in Vietnam. The research was carried out to establish the plant propagation for the purpose of conserving and exploting this endangered medicinal herbs. The young leaf and nodes of Giao co lam in vitro were used as explants in the study to evaluate the factors influencing the multiplication. Young leaf explants were excised and cultured in MS medium with TDZ from 0.1 to 1 mg/L. After 10 weeks of culture, new shoots came out from their explants and the MS medium containing TDZ 0,7 mg/L gave the highest shoots (12.89 shoots/explant) with the average percentage of 74.67%. When nodal explants were cultured on MS supplemented with BA at a concentration of 0.3 to 1.5 mg/L and IBA 0.5 mg/L. After 6 weeks of culture, explants on MS medium supplemented with BA 1 mg/L and IBA 0,5 mg/L gave the highest shoots (7.39 shoots/explant) and their average percentage was 83.33%. In comparison to the nodal explant medium in combination with BA (0.5 to 3 mg/L) and NAA 0.2 mg/L for 4 weeks of culture, the best rapid shoot multiplication score was 3.67 times with MS + BA 1.5 mg/L + NAA 0.2 mg/L as compared to MS + BA 1.0 mg/L + IBA 0.5 mg/L. Suitable medium for rooting was MS + 0.5 mg/L IBA with root shoots at 97.33%, average roots at 5.29 roots/shoot after 4 weeks. On organic substrat, 70% coconut fiber and 30% composted cow manure gave the highest survival rate of 91.33%. The plants grew and developed well during the nursery stage.