Isolation and identification of biological characteristics of porcine circovirus type 2 (PCV2) from pigs in southern Vietnam
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Abstract
his study aimed to isolate and examine the biological characteristics of PCV2 field strains circulating in Vietnam for further study in producing vaccine against PMWS (postweaning multisystemic wasting syndrome). Eighteen PCV2 strains were successfully isolated and belonged to genotype PCV2b (9 strains), PCV2d (6 strains) and recombiant (3 strains). The viral titers of PCV2 isolates at the third passages in PK15A cells were in the range from 1.67 to 5,50 log10TCID50/mL. Three PCV2 field strains with stable viral titers (≥ 5,0 log10TCID50/mL) through passages, which belonged to different genotypes, were selected as master seed for studying of PCV2 vaccines.
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References
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