Identification and antibiogram of Haemophilus parasuis circulating in pig farms in some southern provinces of Vietnam
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Abstract
Haemophilus parasuis (HPS) causes polyserositis in swine and is characterized by pneumonia, pleurisy, peritonitis, cardiomyopathy, arthritis and meningitis. To assess the current status of antibiotic resistance of HPS in industrial pig farms, 245 specimens were collected to isolate HPS on chocolate (PVX) supplemented with NAD. A total of 51/245 specimens had suspected HPS colonies (20.8%) and those colonies were subsequently identified by traditional methods (gram staining and biochemical tests) in combination with PCR using primers specific to a fragment of 275 bp of peptidase M1 gene. Twenty-one colonies (8.6%) were identified as HPS and then were tested for resistance to the nine antibiotics which are popularly used in swine farms. The results of the antibiogram showed that all of these HPS were multiresistant bacteria. The percentage of isolates resistant to 7 types, 6 types and 5 types of antibiotics was 33.33%, 28.6% and 23.8% respectively. Resistance rate was highest forty losin (91%), followed by tilmicosin (81%), tulathromycin (62%), enrofloxacin (62%), lincomycin/spectinomycin (57%), amoxicillin (52%), florfenicol (48%); ceftiofur (10%) and doxycycline (5%). These findings pose a big concernabout the antibiotic resistance of HPS in pigs and that measures should be taken soon to improve this situation.
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References
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