Minimum inhibitory concentration (MIC) determination of herbal extracts against Escherichia coli, Salmonella Typhimurium, and Staphylococcus aureus
,
,
,
,
,
*
, &
Article Sidebar
Main Article Content
Abstract
The objective of this study was to determine the minimum inhibitory concentration (MIC) of crude extract from five medical herbs in Vietnam (Camellia sinensis, Eclipta prostrata L., Pseuderanthemum palatiferum, Psidium guajava, Azadirachta indica) against Escherichia coli ATCC25922, Salmonella Typhimurium, Staphylococcus aureus ATCC25923 by macro - dilution method. These results were the basis for the application of these herbs in the prevention and treatment of diseases in animals. The results showed that MICs of Camellia sinensis against E.coli, S. Typhimurium, and S. aureus were 8 - 16 mg/mL, 8 - 16 mg/mL, 0.5 mg/mL respectively; MICs of Eclipta prostrata L. against E.coli, S. Typhimurium, and S. aureus were 16 mg/mL, 16 mg/mL, 1 - 2 mg/mL respectively; MICs of Pseuderanthemum palatiferum against E.coli, S. Typhimurium and S. aureus were 8 mg/mL, 4 - 8 mg/mL, 2 - 4 mg/mL respectively; MICs of Psidium guajava against E.coli, S. Typhimurium and S. aureus were 16 mg/mL, 16 mg/mL, 0.125 – 0.25 mg/mL respectively and MICs of Azadirachta indica against E.coli, S. Typhimurium, and S. aureus were more than 16 mg/mL.
Article Details
References
Aboulmagd, E., Al-Mohammed, H. I., & Al-Badry, S. (2011). Synergism and posttibiotic effect of green tea extract and imipenem against methicillin resistant Staphylococcus aureus. Microbiology Journal 1(3), 89-96. https://doi.org/10.3923/mj.2011.89.96
Amber, F., Adnan, K., Ilaria, B., Shahana, U. K., Salva-tore, R., & Bianca, P. (2015). Synergistic Antimicro-bial Activity ofCamellia sinensis and Juglans regia against Multidrug-Resistant Bacteria. Plos One 10(2), 1371. https://doi.org/10.1371/journal.pone.0118431
Archana S., & Abraham J. (2011). Comparative analysis of antimicrobial activity of leaf extracts from fresh green tea and black tea on pathogens. Journal of Applied Pharmaceutical Science 1(8), 149-152.
Bongkot, N., Sunpetch, A., Natchaya, P., & Wisarut, M. (2011). Inhibitory and Bactericidal Effects of Pseuderanthemum palatiferum and Piper betle L. Leaves Extracts Against Salmonella species. KKU Veterinary Journal 19(2), 171-179.
Do, B. H. (2006). Medicinal plants and animals in Vietnam 1, 404-419. Ha Noi: Science and Technics Publishing House.
Gustafson, R. H., & Bowen, R. E. (1997). Antibiotic use in animal agriculture. Journal of Applied Microbiology 83(5), 531-541. https://doi.org/10.1046/j.1365-2672.1997.00280.x
Gustafson, R. H., & Bowen, R. E. (1997). Antibioticuse in animal agriculture. Journal of Applied Microbiology 83, 531-541.
Madhab, R., Rafael, A. A., Berna, E., & Conny, R. S. (2013). Use of antibioticts as fed additives: a buring question. Frontiers in microbiology 5, 334. https://doi.org/10.3389/fmicb.2014.00334
Maksum, R., Rafael, A. A., Berna, E., & Conny, R. S. (2013). Antimicrobial activity of green tea extract against isolates of methicillin-resistant Staphylococcus aureus and multi–drug resistant Pseudomonas aeruginosa. Asian Pacific journal of tropical biomedicine 3(8), 663-667.. https://doi.org/10.1016/S2221-1691(13)60133-1
Maragathavalli, S., Brindha, S., Kaviyarasi, N. S., Annadurai, B., & Gangwar, S. K. (2012). Antimicrobial avtivity in leaf extract of neem (Azadirachta indica Linn.). International Journal of Science and Nature 3(1), 110-113.
Nguyen, L. T. K. (2012). Effects of garlic and turmeric on disease resistance and growth performance of 30 to 90-day-old pigs and finisher pigs (Unpublished doctoral dissertation). Nong Lam University, Ho Chi Minh City, Vietnam.
Nguyen, V. Q., & Eun, J. B. (2013). Antimicrobial activity of some Vietnamese medicinal plants extracts. Journal of Medicinal Plants Research 7(35), 2597-2605. https://doi.org/10.5897/JMPR2013.4452
Pandey, M. K., Singh, G. N., Sharma, R. K., & Lata, S. (2011). Antibacterial activity of Eclipta alba (L.) Hassk. Journal of Applied Pharmaceutical Science 1(7), 104-107.
Raja, R. R. Y, Krishna, K. C, Lokanatha, O, Mamatha, S., & Damodar, R. C. (2013). Antimicrobial activity of Azadirachta Indica (neem) leaf, bark and seed extracts. International Journal of Research in Pharmacology and Phytochemistry 3(1), 1-4.
Sanches, N. R., Garcia Cortez, D. A., Schiavini, M. S., Nakamura, C. V., & Dias Filho, B. P. (2005). An Evaluation of Antibacterial Activities of Psidium guajava (L.). Brazilian Archives of Biology and Technology 48(3), 429-436.
Tiwari, R. P., Bharti, S. K., Kaur H. D., Dikshit R. P., & Hoondal G. S. (2005). Synergistic antimicrobial activity of tea & antibioticts. Indian Journal of Medical Research 122(1), 80-84.
Victor, L. (2005). Antibiotics in Laboratory Medicine. Clinical Infectious Diseases 41(4), 577. https://doi.org/10.1086/432067
Vo, A. T. T., Marshall, B. M., Levy, S., Ochieng, D., Gur, D., Nanuashvili, A., Kim, Y. S., Byarugabe, D. K., Okoh, A., Kashyap, S. K., Endtz, H., & Sosa, A. (2010). Surveillance of antibiotic resistance determinants in commensal and environmental bacteria from international sources. Interscience Conference on Antimicrobial Agents and Chemotherapy. Boston, Massachusetts, USA.
Vo, A. T. T., van Duijkeren, E., Fluit A. C., Heck M. E. O. C., Verbruggen A., Maas, H. M. E., & Gaastra, W. (2006). Distribution of Salmonella enterica serovars from humans, livestock and meat in Vietnam and the dominance of Salmonella Typhimurium phage type 90. Veterinary Microbiology 113(1-2), 153-158. https://doi.org/10.1016/j.vetmic.2005.10.034
Wiegand, I., Hilpert, K., & Hancock, R. E. W. (2008). Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nature Protocols 3(2), 163-175. https://doi.org/10.1038/nprot.2007.521