Tan D. Nguyen * , & Thuy M. Nguyen

* Correspondence: Nguyen Duy Tan (email: ndtan@agu.edu.vn)

Main Article Content


Pouzolzia zeylanica is a kind of medicinal plant which is generally cultivated in Mekong Delta region. It owns many bioactive compounds that are known to possess antioxidant, antimicrobial and anticarcinogenic properties. This study aimed to optimize additional carrier concentration for spray drying of Pouzolzia zeylanica extract. Response Surface Methodology (RSM) with central composite design (CCD) was applied for optimization and investigation of the influence of maltodextrin (5÷15%, w/v) and carrageenan gum (0.06÷1.0%, w/v) concentration on the physicochemical characteristics of spray dried powder (bioactive compounds, moisture content as well as particle size distribution). The results showed that the optimum concentrations of maltodextrin and carrageenan gum were 8.8% w/v and 0.082% w/v, respectively. At these optimal conditions, the anthocyanin, flavonoid, polyphenol, tannin, moisture content and particle size of obtained spray dried powder were 5.77 mg cyanidin-3-glycoside equivalents (CE)/100 g; 29.49 mg quercetin equivalents (QE)/g; 28.35mg gallic acid equivalents (GAE)/g; 27.44 mg tannic acid equivalents (TAE)/g, 6.55% and 6.09 µm, respectively.
Keywords: Carragennan, Maltodextrin, Pouzolzia zeylanica, Response Surface Methodology, Spray drying

Article Details


Abadio, F. D. B., Domingues, A. M., Borges, S. V., & Oliveira, V. M. (2004). Physical properties of powdered pineapple (Ananas comosus) juice-effect of maltodextrin concentration and atomization speed. Journal Food Engineering 64(3), 285-287. https://doi.org/10.1016/j.jfoodeng.2003.10.010

Ahmed, J. K., Salih, H. A. M., & Hadi, A. G. (2005). Anthocyanins in red beet juice act as scavengers for heavy metals ions such as lead and cadmium. International Journal of Science and Technology 2(3), 269-274.

Bhusari, S. N., & Kumar, P. (2014). Antioxidant activities of spray dried tamarind pulp powder as affected by carrier type and their addition rate. International Conference on Food, Biological and Medical Sciences (FBMS-2014), Bangkok (Thailand).

Burey, P., Bhandari, B. R., Howes, T., & Gidley, M. J. (2008). Hydrocolloid gel particles: Formation, characterization, and application. Cirtical Reviews in Food Sciences and Nutrition 48(5), 361-377. https://doi.org/10.1080/10408390701347801

Eswari, M. L., Bharathi, R. V., & Jayshree, N. (2013). Preliminary phytochemical screening and heavy metal analysis of leaf extracts of Ziziphus oenoplia (L) Mill. Gard. International Journal of Pharmaceutical Sciences and Drug Research 5(1), 38-40.

Favaro, T. C. S., Santana, A. D. S., Monterrey, Q. E. S., Trindade, M. A., & Netto, F. M. (2010). The use of spray drying technology to reduce bitter taste of casein hydrolysate. Food hydrocolloids, 24(4), 336-340. https://doi.org/10.1016/j.foodhyd.2009.10.012

Fernandes, L. P., Candido, R. C., & Oliveira, W. P. (2012). Spray drying micro-encapsulation of Lippia sidoides extracts in carbohyrate blends. Food and Bioproducts Processing 90(3), 425-432. https://doi.org/10.1016/j.fbp.2011.12.001

Fu, M., Niu, Y. Y., Yu, J., & Kong, Q. T. (2012). Study on the chemical constituents in Pouzolzia zeylanica. Zhong Yao Cai 35(11), 1778-17781.

Ghani. (2003). Medicinal plants of Bangladesh: Chemical constituents and uses (2nd ed.). Dhaka, Bangladesh: The Asiatic Society of Bangladesh.

Goula, A. M., Adamopoulos, K. G., & Kazakis, N. A. (2004). Influnce of spray drying conditions on tomato powder properties. Drying Technology 22(5), 1129-1151. https://doi.org/10.1081/DRT-120038584

Goula, A. M., & Adamopoulos, K. G. (2005). Spray drying of tomato pulp in dehumidified air: II. The effect on powder properties. Journal of Food Engineering 66(1), 35-42. https://doi.org/10.1016/j.jfoodeng.2004.02.031

Grabowski, J. A., Truong, V. D., & Daubert, C. R. (2006). Spray-drying of amylase hydrolyzed sweet potato puree and physicochemical properties of powder. Journal of Food Science 71(5), 209-217. https://doi.org/10.1111/j.1750-3841.2006.00036.x

Guan, X., & Yao, H. (2008). Optimization of viscozyme L assisted extraction of oat bran protein using response surface methodology. Food Chemistry 106(1), 345-351. https://doi.org/10.1016/j.foodchem.2007.05.041

Hossain, M.A., AL-Raqmi, K. A. S., AL-Mijizy, Z. H., Weli, A. M., & Al-Riyami, Q. (2013). Study of total phenol, flavonoids contents and phytochemical sreening of various leaves crude extracts of locally grown Thymus vularis. Asian Pacific Journal of Tropical Biomedicine 3(9), 705-710. https://doi.org/10.1016/S2221-1691(13)60142-2

Kha, T. C., Nguyen, M. H., & Roach, P. D. (2010). Effects of spray drying conditions on the physicochemical and antioxidant properties of the Gac (Momordica cochinchinensis) fruit aril powder. Journal of Food Engineering 98(3), 385-392. https://doi.org/10.1016/j.jfoodeng.2010.01.016

Laitonjam, W. S., Yumnam, R., Asem, S. D., & Wangkheirakpam, S. D. (2013). Evaluative and comparative study of biochemical, trace elements and antioxidant activity of Phlogacanthus pubinervius T. Anderson and Phlocanthus jenkincii C.B. Clarke leaves. Indian Journal of Natural Products and Resources 4(1), 67-72.

Langrish, T. A. G., Chan, W. C., & Koda, K. (2007). Comparison of maltodextrin and skim milk wall deposition rates in a pilot-scale spra dryer. Powder Technology 179(1-2), 83-89. https://doi.org/10.1016/j.powtec.2007.01.019

Le, T. T. (2007). Preliminary test of chemical components for Pouzolzia zeylanica L. Benn. (Unpublished master’s thesis). University of Natural Science, HCM city, Vietnam.

Mandal, S., Patra, A., Samanta, A., Roy, S., Mandal, A., Mahapatra, T. D., Pradhan, S., Das, K., & Nandi, D. K. (2013). Analysis of phytochemical profile of Terminalia arjuna bark extract with antioxidative and antimicrobial properties. Asian Pacific Journal of Tropical Biomedicine 3(12), 960-966. https://doi.org/10.1016/S2221-1691(13)60186-0

Martineli, L., Gabas, A. L., & Telis-Romero, J. (2007). Thermodynamic and quality properties of lemon juice powder as affected by maltodextrin and arabic gum. Drying Technology 25(12), 2035-2045. https://doi.org/10.1080/07373930701728836

Masniza, M., Hani, I. M. H., Farah, S. H., & Rinani, S. A. R. (2013). Maltodextrin encapsulation of Garcinia atroviridis Griff by spray drying technique. International Journal of Scientific Engineering and Technology 2(10), 1011-1012.

Myers, R. H., Montgomery, D. C., & Anderson-Cook, C. M. (2009). Response surface methodology: process and product optimization using designed experiments. Wiley: New York.

Ochoa-Martinez, L. A., Garza-Juarez, S. E., Rocha- Guzman, N. E., Moraies-Castro, J., & Gonzalez-Herrera, S. M. (2015). Functional properties, color and betala in content in beetroot-orange juice powder obtained by spray drying. Journal of Food and Dairy Technology 3(2), 30-36.

Oktay, M., Gülçin, İ., & Küfrevioğlu, Ö. İ. (2003). Determination of in vitro antioxidant activity of fennel (Foeniculum vulgare) seed extracts. LWT-Food Science and Technology, 36(2), 263-271. https://doi.org/10.1016/S0023-6438(02)00226-8

Sabhadinde, V. N. (2014). The physicochemical and storage properties of spray dried orange juice powder. Indian Journal of Fundamental and applied life science 4(4), 153-159.

Saha, D., & Paul, S. (2012). Studies on Pouzolzia zeylanica (L.) Benn. (Family: Urticaceae). Oxford, United Kingdom: LAP LAMBERT Academic Publishing.

Sansone, F., Mencherini, T., Picerno, P., d’Amore, M., Aquino, R. P., & Lauro, M. R. (2011). Maltodextrin/pectin microparticles by spray drying as carrier for nutraceutical extracts. Journal of Food Engineering 105(3), 468-476. https://doi.org/10.1016/j.jfoodeng.2011.03.004

Santana, A., Kuroxawa, L., Oliveria, R., & Park, K. (2016). Spray drying of pequi pulp: process performance and physicochemical and nutritional properties of the powdered pulp. Brazlian Archives of Biology and Technology 59, 1-11. https://doi.org/10.1590/1678-4324-2016150362

Santos, D. T., Cavalcanti, R. N., Rostagno, M. A., Queiroga, C. L., Eberlin, M. N., & Meireles, M. A. A. (2013). Extraction of polyphenols and anthocyanins from the Jambul (Syzygium cumini ) fruit peels. Food and Public Health 3(1), 12-20. https://doi.org/10.5923/j.fph.20130301.02

Sarabandi, K., Peighambardoust, S. H., & Shirmohammadi, M. (2014). Physical properties of spray dried grape syrup as affected by drying temperature and drying aids. International Journal of Agriculture and Crop Sciences 7(12), 928-934.

Shari, A., Niakousari, M., Maskooki, A., & Mortazavi, S. A. (2015). Effect of spray drying conditions on the physicochemical properties of barberry (Berberis vulgaris) extract powder. International Food Research Journal 22(6), 2364-2370.

Shishir, M. R. I., Tuip, F. S., Aziz, N. A., Taih, R. A., & Safulah, M. (2015). Effect of maltodextrin concentrations at different drying temperatures on the physical and drying properties of the spray dried pink guava powder. Journal of Applied Science and Agriculture 10(5), 176-182.

Singh, V. K., Pandey, S., Pare, A., & Singh, R. B. (2014). Optimization of process parameters for the production of spray dried Ber (Ziziphus jujube L.) powder. Journal Food Science Technology 51(12), 3956-3962. https://doi.org/10.1007/s13197-012-0897-3

Spigno, G., Tramelli, L., & De-Faveri, D. M. (2007). Effects of extraction time, temperature and solvent on concentration and antioxidant activity of grape marc phenolics. Journal of Food Engineering 81(1), 200-208. https://doi.org/10.1016/j.jfoodeng.2006.10.021

Tee, L. H., Lugman, C. A., Pin, K. Y., Addull, R. A., & Yusof, Y. A. (2012). Optimization of spray drying process parameters of Piper betle L. (Sirih) leaves extract coated with maltodextrin. Journal of Chemical and Pharmaceutical Research 4(3), 1833-1841.

Tonon, R. V., Brabet, C., & Hubinger, M. D. (2008). Influnce of process conditions on the physicaochemical properties of Acai (Euterpe Oleraceae Mart.) powder produced by spray drying. Journal of Food Engineering 88(3), 411-418. https://doi.org/10.1016/j.jfoodeng.2008.02.029

Tran, T. M., Nguyen, H. T. T., Duong, N. T. M., Tran, L. C., & La, K. V. (2010) (2010). Experimental study on some pharmacological effects of Pouzolzia zeylanica and Tinospora crispa. Medicine Journal of Ho Chi Minh city, Special Subject Traditional Medicine 14 (2), 116-120.

Tsai, C. W., Tong, L. I., & Wang, C. H. (2010). Optimization of multiple responses using data envelopment analysis and response surface methodology. Tamkang Journal of Science and Engineering 13(2), 197-203. https://doi.org/10.6180/jase.2010.13.2.10

Vo, C. V. (2012). Dictionary medicinal plants in Vietnam. Ha Noi, Viet Nam: Medicine Publishing House.

Wang, W., & Zhou, W. (2013). Effect of maltodextrin on water adsorption and glass transition of dried soy sauce powder. Food and Bioprocess Technology. Doi 10(5), 11947-12012

Wangensteen, H., Samuelsen, A. B., & Malterud, K. E. (2004). Antioxidant activity in extracts from coriander. Food Chemistry 88(2), 293-297. https://doi.org/10.1016/j.foodchem.2004.01.047

Zabeti, M., Daud, W. M. A., & Aroua, M. K. (2009). Optimization of the activity of CaO/Al 2 O 3 catalyst for biodiesel production using response surface methodology. Applied Catalysic A: General 366(1), 154-159. https://doi.org/10.1016/j.apcata.2009.06.047