Effects of growth media on growth and commercialisable percentage of disease-free cassava cultivar KM140 (Manihot esculenta Crantz “KM140”)
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Abstract
Cassava mosaic disease (CMD) is one of the most serious diseases that has caused heavy losses in starch yield. In vitro propagation from CMD disease-free cassava is the optimal method to produce healthy seedlings. Identification of a suitable growth medium for the development of ex vitro plantlets during the acclimation stage is an important step in order to obtain healthy plant. In this study, common substrates such as coir, rice husk ash, and vermicompost were mixed in different proportions to create formulations for the autotrophic stage of disease-free KM140 cassava plants. The parameters measured including plant height, number of leaves, stem diameter, number of branches, root length, dry matter ratio of leaves, roots, survival rate, and percentage of nurse stage cassava plantlets were evaluated. The results showed that cassava plants were transferred from in vitro to ex vitro environments gained a survival rate of 84.5% in coir substrate after 1 week. The growth medium contained a mix of 85% coir + 10% rice husk ash + 5% vermicompost was suitable for the growth and development of KM140 cassava plantlets. In this condition, the plantlets reached height of 38.8 cm with 22.0 leaves and the percentage of commercialisable plantlets reached 85.3%.
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Bonilla Morales, M. M., Sánchez Ordoñez, S. A., & Pachón García, J. (2015). Evaluation of organic substrates for acclimatization and hardening of vitroplants of cassava (Manihot esculenta Crantz). Revista de Investigación Agraria y Ambiental 6(2), 31-36.
DPP (Department of Plant Protection). (2020). Report on Cassava Mosaic Disease (CMD) situation. Meeting of Steering Committee for prevention and control of CMD, November 24, 2020 in Tay Ninh. Retrieved May 19, 2021, from https://www.ppd.gov.vn/tin-moi-nhat-289/hop-ban-chi-dao-phong-chong-benh-kham-la-san.html
Escobar, R., Restrepo, J., Tohme, J. M., & Roca, W. (2013a). Use of tissue culture in cassava for rural households in Colombia. In Ruane, J., Dargie, J. D., Mba, C., Boettcher, P., Makkar, H. P. S., Barthey, D. M., & Sonnino, A. (Eds). Biotechnologies at work for smallholders: Case studies from developing countries in crops, livestock and fish (56-62). Rome, Italy: Food and Agriculture Organization of the United Nations (FAO).
Hamill, S. D., 2014. Processes, costs and traits of plants produced in tissue culture must be considered to develop effective crop production systems. In Lambardi, M., Hamill, S., & Drew, R. (Eds.). 1113 XXIX International Horticultural Congress on Horticulture: Sustaining Lives, Livelihoods and Landscapes (IHC2014): International Symposium on Micropropagation and In Vitro Techniques (85-92). Bierbeek, Belgium: ISHS Acta Horticulturae. https://doi.org/10.17660/ActaHortic.2016.1113.12
Hillocks, R. J., Thresh, J. M., & Bellotti, A. (2002). Cassava: biology, production and utilization. New York, USA: CABI Publishing.
Hy, N. H., Reinhardt, H., Nhan, P. T. & Buu, B. C. (2020). Cassava science. Hanoi, Vietnam: Agricultural publisher.
Iwuagwu, M. O., & Nwosu, N. N. (2018). Performance of in vitro cassava (Manihot esculenta Crantz) plantlets weaned with locally sourced substrates. International Journal of Environment, Agriculture and Biotechnology 3(2), 664-669. http://dx.doi.org/10.22161/ijeab/3.2.47
Lebot, V. (2009). Tropical Root and Tuber Crops: Cassava, Sweet Potato, Yams, Aroids (2nd ed.). Oxfordshire, UK: CABI Publishing.
Naranjo, C., & Fallas, E. (2017, April). Ex vitro establishment and macropropagation of cassava (Manihot esculenta 'Valencia') to obtain disease-free rooted plants. In Paiva, R., Reis, M. V., & Silva, D. P. C. (Eds.). 1224 VII International Symposium on Production and Establishment of Micropropagated Plants (217-220). Bierbeek, Belgium: ISHS Acta Horticulturae. https://doi.org/10.17660/ActaHortic.2018.1224.29
Thresh, J. M., & Cooter, R. J. (2005). Strategies for controlling cassava mosaic virus disease in Africa. Plant pathology 54(5), 587-614. https://doi.org/10.1111/j.1365-3059.2005.01282.x
Thresh, J. M., Otim-Nape, G. W., Legg, J. P., & Fargette D. (1997). Africa cassava mosaic disease: What is the magnitude of the problem?. In Thro, A. M., & Akoroda, M. O. (Eds). Proceedings of cassava Biotechnology network, Third International scientific meeting, Kampala, Uganda 1996, 26–31 August 1996 (13-19).
Ubalua, A. O., & Nsofor, G. C. (2017). The role of supporting substrates in ex vitro acclimatization and growth of tissue cultured cassava plantlets. Plant Knowledge Journal 6(1), 1-6.
Uke, A., Hoat, T. X., Quan, M. V., Liem, N. V., Ugaki, M., & Natsuaki, K. T. (2018). First Report of Sri Lankan cassava mosaic virus Infecting Cassava in Vietnam. Plant Disease 102(12). 2669. https://doi.org/10.1094/PDIS-05-18-0805-PDN