Antibiotic resistance of Vibrio spp. isolated from white-leg shrimp (Litopenaeus vannamei ) collected from Binh Dien Wholesale Market
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Abstract
The study was conducted to determine the levels of antibiotic resistance and multiple antibiotic resistance of 150 Vibrio spp. isolates from white-leg shrimp (Litopenaeus vannamei) collected from Binh Dien Wholesale Market in Ho Chi Minh City. Ten antibiotics were used to test the resistance of Vibrio ssp. isolates including ampicillin, ciprofloxacin, chloramphenicol, doxycycline, gentamicin, kanamycin, nalidixic acid, streptomycin, tetracycline, and trimethoprim/sulfamethoxazole. Antibiotic susceptibility test results showed that the percentage of Vibrio spp. resistance to the above antibiotics was 92; 12; 0; 0; 3.3; 80; 3.3; 46.7; 3.3 và 18.0%, respectively. The percentage of multiple resistant isolates from two to five tested antibiotics was 88.7%. Especially, none of the isolates were sensitive to all tested antibiotics. The multiple antibiotic resistance (MAR) index value was 0.259 indicating that these isolates were exposed to high-risk sources of contamination where antibiotics were commonly used.
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Ashash, U., Noach, C., Perelman, B., Costello, C., Sansalone, P., Brazil, T., & Raviv, Z. (2019). In ovo and day of hatch application of a live infectious bursal disease virus vaccine to commercial broilers. Avian Diseases 63(4), 713-720. https://doi.org/10.1637/aviandiseases-D-19-00087.
Bui, T. K., Bui, H. K., & Bui, T. K. (2001). Antibiotics. Department of Science and Technology Ba Ria - Vung Tau province.
Chikwendu, C. I., Ibe, N. S. N., & Okpokwasili, C. G. (2014). Multiple antimicrobial resistance in Vibrio spp. isolated from river and aquaculture water sources in Imo State, Nigeria. British Microbiology Research Journal 4(5), 560-569.
Chowdhury, G., Pazhani, G. P., Dutta, D., Guin, S. Dutta, S., Ghosh, S., Izumiya, H., Asakura, M., Yamasaki, S., Takeda, Y., Arakawa, E., Watanabe, H., Mukhopadhyay, A. K., Bhattacharya, M. K., Rajendran, K., Nair, G. B., & Ramamurthy, T. (2012). Vibrio fluvialis in patients with diarrhea, Kolkata, India. Emerging Infectious Diseases 18(11), 1868-1871. 10.3201/eid1811.120520
CLSI (Clinical and Laboratory Standards Institute). (2012). Performance standards for antimicrobial susceptibility testing; Twenty Second Informational Sup- plement, M100-S22, Vol. 32 No. 3, Replaces M100- S21, Vol. 31 No. 1 (Clinical and Laboratory Standards Institute). Retrieved September 1, 2018, from http://zums.ac.ir/files/health/pages/ill/azmayeshgha h/clsi_2013.pdf
Dang, O. T. H., Doan, P. N., Nguyen, H. T. T., & Nguyen. P.T. (2006). Determine the classification and antibiotic resistance of glowing Vibrio isolated from the post larvae of the tiger shrimp (Penaeus monodon). Journal of Can Tho University 4, 42-52.
Dang, O. T. H., Nguyen, P. T. , Somsiri, T., Chinabut, S., Yusoff, F., Shariff, M., Bartie, K., , Giacomini, M., Bertone, S., Swings, J., & Teale, A. (2005). Antibiotic susceptibility testing of aquaculture associated bacteria originating from integrated farming systems in the Mekong River Delta, Viet Nam. Journal of Can Tho University 4, 136-144.
Ho, D. K., Truyen, H. N. D., & Luu, T. T. T. (2019). Antibiotic resistance of bacteria of Vibrio spp. in whiteleg shrimp (Litopenaeus vannamei). Journal of Fisheries Science and Technology Nha Trang University 4, 26-32.
Huynh, T. N., Tran, T. T. N., & Nguyen, D. T. (2015). Prevalence and antibiotic resistance pattern of Vibrio spp. isolated from aquaculture and environment in Tien Giang province. Ho Chi Minh City University of Education Journal of Science 2(67), 157-167
Krumperman, P. H. (1983). Multiple antibiotic resistance indexing of Escherichia coli to identify high-risk sources of fecal contamination of foods. Applied and Environmental Microbiology 46(1), 165-170. https://doi.org/10.1128/aem.46.1.165-170.1983
Le, P. H., Nguyen, T. D., Hua, P. N., & Pham, Y. T. (2018). Current status of antibiotic usage in black tiger shrimp and white leg shrimp farming in Vietnam. Journal of Mekong Fisheries 11, 10-23.
Manjusha, S., Sarita, G. B., Elyas, K. K., & Chandrasekaran, M. (2005). Multiple antibiotic resistances of Vibrio isolates from coastal and brackish water areas. American Journal of Biochemistry and Biotechnology 1(4), 201-206.
MARD (Ministry of Agriculture and Rural Development) (2016). Decision No 10/2016/TT-BNNPTNT date on June 01, 2016. List of veterinary drugs permitted to be marketed and banned from use in Vietnam. Retrieved June 27, 2021, from http://vanban.chinhphu.vn/portal/page/portal/chinhphu/hethongvanban?Class_id=1&mode=detail&document_id=186403
Nguyen, A. T. T., & Vo, N. V. (2016). Prevalence and antibiotic susceptibility of Vibrio parahaemolyticus isolated from lobster (Panulirus ornatus) in cage culture in Phu Yen province. Journal of Veterinary science and Technology XXIII(2), 41-46.
Nguyen, D. T., Nguyen, L. T., Ho, T. T. V., & Ha, T. T. (2014a). Prevalence and antibiotic resistance of Vibrio spp. isolated from swine blood sample, clam and patient with diarrhea in Tra Vinh Province. Journal of Can Tho University 33, 61-67.
Nguyen, K. H. N. (2012). Molecular characterisation of antibiotic resistant bacteria isolated from farmed catfish and humans in vietnam. Victoria, Australia: RMIT University.
Nguyen, T. C., Tran, L. T. N., & Huynh, N. T. Q. (2019). Antibiotics resistance abilities of Vibrio parahaemolyticus bacteria that isolated from white leg shrimp (Litopenaeus vannamei) farming areas in Bac Lieu province in 2019. Journal of Fisheries Science and Technology Nha Trang University 4, 139-147.
Nguyen, T. Q., Mssashi, M., & Tran, P. M. (2020). Chemical use in intensive whiteleg shrimp aquaculture in Tra Vinh province, Vietnam. Journal of Can Tho University 2, 70-77.
Nguyen, T. T., Nguyen, K. P., & Phan, N. T. H. (2014b). Contamination of Vibrio spp. on Penaeus merguiensis, Penaeus monodon, Penaeus ensis in some markets in Ninh Kieu District, Cantho City. Journal of Can Tho University 2, 111-115.
Pham, H. (2021). Shrimp industry overview 2020: Growth in difficulties. Retrieved Feb 18, 2021, from http://nguoinuoitom.vn/tong-quan-nganh-tom-2020-tang-truong-trong-kho-khan/
Phan, T. T. A., Doan, K. T. D., & Nguyen, T. C. (2019). The status of antibiotics resistance of Vibrio spp. that isolated from white leg shrimp (Litopenaeus vannamei) farming pond in Ben Tre province. Journal of Fisheries Science and Technology Nha Trang University 4, 122-129.
Raissy, M., Moumeni, M., Ansari, M., & Rahimi, E. (2012). Antibiotic resistance pattern of some Vibrio strains isolated from seafood. Iranian Journal of Fisheries Sciences 11(3), 618-626.
Robert, M. C. (1996). Tetracycline resistance determinants: mechanisms of action, regulation of expression, genetic mobility, and distribution. FEMS Microbiology Reviews 19(1), 1-24. https://doi.org/10.1111/j.1574-6976.1996.tb00251.x
Serrano, P. H. (2005). Responsible use of antibiotics in aquaculture. Food and Agriculture Organization of the United Nations, Fisheries Technical.
Tendencia, E. A., & de la Peña, L. D. (2001). Antibiotic resistance of bacteria from shrimp ponds. Aquaculture 195(3-4), 193-204. https://doi.org/10.1016/S0044-8486(00)00570-6
Tolmasky, M. E. (2000). Bacterial resistance to aminoglycosides and β-lactams: The Tn1331 transposon paradigm. Frontiers in Biology Science 1(5), 20-29. https://doi.org/10.2741/tolmasky
Truong, H. T. M., Pham, Y. T., Huynh, L. T. M., Phan, V. T., Nguyen, V. D., & Truong, V. T. T. (2016). Current status of antibiotic usages and resistance of Vibrio parahaemolyticus causing acute hepatopancreatic necrosis disease on shrimp in Quynh Luu, Nghe An. Journal of Fisheries Science and Technology Nha Trang University 4, 57-64.