Determination of Riemerella anatipestifer and Escherichia coli suspected to cause a syndrome including shaking head and trembling, greenish diarrhea on duck farms in Long An
,
,
,
,
, &
Article Sidebar
Main Article Content
Abstract
In Long An province and other places, ducks at 3-8 weeks of age often experience a syndrome including greenish diarrhea, eye and nose discharge, shaking head and trembling which is considered to be related to Riemerella anatipestifer and Escherichia coli. There is not any research to prove this hypothesis. For that reason, this study was as the first step to explore the causes of this syndrome. In to-tal, 70 swab samples from disease and nondisease ducks and 27 pool water from 27 duck farms were collected to identify R. anatipestifer and E. coliusing PCR. There were 2 PCR (16SrDNA-PCR and gyrB-PCR) used to detect R. anatipestifer while one PCR for gene phoA was used to detect E. coli The result showed that R. anatipes-tifer was found on both disease and non-disease ducks. However, the 2 PCRs to detect R. anatipestifer did not agree well. The fact that these bacteria were found on 22% farms (disease and non-diseaseones) means it is complicated to confirm them in causing the men-tioned disease. In addition,E. coliwas also found in both types of duck and from all pool water. These isolates were kept for further studies to identify their virulence. The result reveals that there may be the cooperation between many factors in causing this disease.
Article Details
References
Bui, D. H., Do, D. T., Nguyen, T. T., Nguyen, N. T. T., Le, H. T., & Nguyen, N. T. P. (2016). Determination of duck circovirus (DuCV) and Riemerella anatipestifer (RA) in several cases of septicemia disease from duck flocks by PCR technique. Journal of Veterinary Science and Technology 6, 14-21.
Christensen, H., & Bisgaard, M. (2010). Phylogenetic relationships of Riemerella anatipestifer serovars and related taxa and an evaluation of specific PCR tests reported for R. anatipestifer. Journal of Applied Microbiology 108(5), 1612-1619. https://doi.org/10.1111/j.1365-2672.2009.04558.x
Christensen, J. P. (2013). Overview of Riemerella anatipestifer infection in poultry. Veterinary manual. Retrieved July 12, 2013, from
https://www.msdvetmanual.com/poultry/riemerellaanatipestifer-infection/overview-of-riemerellaanatipestifer-infection-in-poultry.
Glisson, J. R., Charles, L. H., & Jens, P. C. (2013). Fowl Cholera. In Swayne, D. E., Glisson, J. R., McDougald, L. R., Nolan, L. K., Suarez, D. L., & Nair, V. L. (Eds). Diseases of Poultry (13rd ed., 807-823). New Jersey, USA: Wiley-Blackwell.
Kardos, G., Nagy, J., Antal, M., Bistyák, A., Tenk, M., & Kiss, I. (2007). Development of a novel PCR assay specific for Riemerella anatipestifer. Letters in Applied Microbiology 44(2), 145-148. https://doi.org/10.1111/j.1472-765X.2006.02053.x
Nolan, L. K., John, H. B., Jean-Pierre, V., Tahseen, A., & Catherine, M. L. (2013). Colibacillosis. In Swayne, D. E., Glisson, J. R., McDougald, L. R., Nolan, L. K., Suarez, D. L., & Nair, V. L. (Eds). Diseases of Poultry (13nd ed., 751-805). New Jersey, USA: WileyBlackwell.
Rubbenstroth, D., Ryll, M., Knobloch, J. K. M., Kohler, B., & Rautenschlein, S. (2013). Evaluation of different diagnostic tools for the detection and identification of Riemerella anatipestifer. Avian Pathology 42(1), 1726. https://doi.org/10.1080/03079457.2012.752066
Wang, X. P., Zhu, D. K., Wang, M. S., Cheng, A. C., Jia, R. Y., Chen, S., Chen, X. Y., & Tang, T. (2012). Development and application of specific polymerase chain reaction assay targeting the gyrB gene for rapid detection of Riemerella anatipestifer. Poultry Science 91(10), 2450-2453. https://doi.org/10.3382/ps.2012-02375
Wei, B., Cha, S. Y., Kang, M., Park, I. J., Moon, O. K., Park, C. K., & Jang, H. K. (2013). Development and application of a multiplex PCR assay for rapid detection of 4 major bacterial pathogens in ducks. Poultry Science 92(5), 1164-1170. https://doi.org/10.3382/ps.2012-02823