Trinh T. N. Huynh * , An T. T. Vo , Phuong T. Y. Nguyen , & Cuong V. Nguyen

* Correspondence: Huynh Thi Ngoc Trinh (email: huynhngoctrinht39@gmail.com)

Main Article Content

Abstract

This study was conducted to determine the diversity of pathogenic Vibrio species, the antimicrobial resistance profile and the presence of virulence genes linked to food-borne pathogens of Vibrio spp. isolated from shrimp samples in Ho Chi Minh City, Vietnam. A total of 40 raw shrimp batches were collected from retails markets (supermarket and street). All 133 test strains were isolated from 40 shrimp samples. V. parahaemolyticus was the most common species (87.5%), followed by V. navarrensis (60%), V. alginolyticus (52.5%), V. cholerae non-O1 (37.5%), V. vulnificus (22.5%), and V. fluvialis (10%). Vibrio spp. isolates were susceptible to 12 antimicrobial agents. The prevalence of ampicillin resistance was highest (82.7%), followed by cotrimoxazole (18.8%) and 3rd generation cephalosporins (16.5% cefotaxime and 8.3% ceftazidime). Extended spectrum β lactamase (ESBL) activity was detected in 28.1% V. parahaemolyticus isolates. None of tdh or trh virulence genes were detected. The results of this study indicated the presentation of Vibrio species in shrimp samples purchased in Ho Chi Minh City. Therefore, our results could be of great potential for the identification of Vibrio infection in shrimp samples taken from different regions to improve food quality and safety.  

Keywords: Vibrio, Shrimp, Prevalence, Antimicrobial resistance, Virulence genes

Article Details

References

Asgarpoor, D., Haghi, F., & Zeighami, H. (2018). Detection and molecular characterization of Vibrio Parahaemolyticus in shrimp samples. The Open Biotechnology Journal 12, 46-50.

Baker-Austin, C., Oliver, J. D., Alam, M., Ali, A., Waldor, M. K., Qadri, F., & Martinez-Urtaza, J. (2018). Vibrio spp. infections. Nature Reviews Disease Primers 4(1), 1-19.

Baker-Austin, C., Trinanes, J., Gonzalez Escalona, N., & Martinez-Urtaza, J. (2017). Non-Cholera Vibrios: The microbial barometer of climate change. Trends in Microbiology 25, 76-84.

Chowdhury, A., Ishibashi, M., Thiem, V. D., Tuyet, D. T. N., Tung, T. V., Chien, B. T., Seidlein, L. V., Canh, D. G., Clemens, J., Trach, D. D., & Nishibuchi, M. (2004). Emergence and serovar transition of Vibrio parahaemolyticus pandemic strains isolated during a diarrhea outbreak in Vietnam between 1997 and 1999. Microbiology and Immunology 48, 319-327.

CLSI (Clinical and Laboratory Standards Institude). (2016). Methods for antimicrobial dilution and disk susceptibility testing of infrequently isolated or fastidious bacteria (3rd ed.). Wayne, USA: Clinical and Laboratory Standards Institute.

Cornejo-Granados, F., Lopez-Zavala, A. A., Gallardo- Becerra, L., Mendoza-Vargas, A., S ́anchez, F., Vichido, R., Brieba, L. G., Viana, M. T., Sotelo-Mundo, R. R., & Ochoa-Leyva, A. (2017). Microbiome of pacific whiteleg shrimp reveals differential bacterial community composition between wild, aquacultured outbreak conditions. Scientific Reports 7, 1-15.

de Melo, L. M. R., Almeida, D., Hofer, E., Dos Reis, C. M. F., Theophilo, G. N. D., Santos, A. F. das M., & Vieira, R. H. S. D. F. (2011). Antibiotic resistance of Vibrio parahaemolyticus isolated from pond-reared Litopenaeus vannamei marketed in natal, brazil. Brazilian Journal of Microbiology 42(4), 1463-1469.

Diep, T. T., Au, T. V., Nguyen, N. T. N., Nguyen, N. T. K., & Nguyen, L. T. P. (2011). Virulence and antimicrobial resistance characteristics of Vibrio parahaemolyticus isolated from environment, food and clinical samples in the south of Vietnam, 2010. BMC Proceedings 5 (Suppl 1), 94.

Elmahdi, S., DaSilva, L. V., & Parveen, S. (2016). Antibiotic resistance of Vibrio parahaemolyticus and Vibrio vulnificus in various countries: A review. Food Microbiology 57, 128-134.

Giang, H. (2017). Action plan to develop antimicrobial resistance phenotype and genotype of pathogenic Vibrio parahaemolyticus isolated from seafood. Food Control 59, 207–211.

Gopal, S., Otta, S. K., Kumar, S., Karunasagar, I., Nishibuchi, M., & Karunasagar, I. (2005). The occurrence of Vibrio species in tropical shrimp culture environments; implications for food safety. International Journal of Food Micobiology 102(2), 151-159.

Joseph, S. W., Colwell, R. R., & Kaper, J. B. (1982). Vibrio parahaemolyticus and related halophilic Vibrios. CRC Critical Reviews in Microbiology 10(1), 77-124.

Koralage, M. S. G., Alter, T., Pichpol, D., Strauch, E., Zessin, K. H., & Huehn, S. (2012). Prevalence and molecular characteristics of Vibrio spp. isolated from preharvest shrimp of the North Western province of Sri Lanka. Journal of Food Protection 75(10), 1846-1850.

Liu, M., Wong, M. H. Y., & Chen, S. (2013). Molecular characterisation of a multidrug resistance conjugative plasmid from Vibrio parahaemolyticus. International Journal of Antimicrobial Agents 42(6), 575-579.

Livermore, D. M., & Woodford, N. (2006). The β - lactamase threat in Enterobacteriaceae, Pseudomonas and Acinetobacter. Trends in Microbiology 14(9), 413- 420.

Lou, Y., Liu, H., Zhang, Z., Pan, Y., & Zhao, Y. (2016). Mismatch between antimicrobial resistance phenotype and genotype of pathogenic Vibrio parahaemolyticus isolated from seafood. Food Control 59, 207-211.

Otta, & Karunasagar, I. (2001). Bacteriological study of shrimp, Penaeus monodon Fabricius, hatcheries in India. Journal of Applied Ichthyology 17(2), 59-63.

Ottaviani, D., Leoni, F., Serra, R., Serracca, L., Decastelli, L., Rocchegiani, E., Masini, L., Canonico, C., Talevi, G., & Carraturo, A. (2012). Nontoxigenic Vibrio parahaemolyticus strains causing acute gastroenteritis. Journal of Clinical Microbiology 50(12), 4141- 4143.

Rocha, R. D. S., Sousa, O. V., Vieira, R. H. (2016). Multidrug-resistant Vibrio associated with an estuary affected by shrimp farming in North Eastern Brazil. Marine Pollution Bulletin 105(1), 337-340.

Shirai, H., Ito, H., Hirayama, T., Nakamoto, Y., Nakabayashi, N., Kumagai, K., Takeda, Y., & Nishibuchi, M. (1990). Molecular epidemiologic evidence for association of thermostable direct hemolysin (TDH) and TDH-related hemolysin of Vibrio parahaemolyticus with gastroenteritis. Infection and Immunity 58(11), 3568–3573.

Sperling, L., Alter, T., & Huehn, S. (2015). Prevalence and antimicrobial resistance of Vibrio spp. in retail and farm shrimps in Ecuador. Journal of Food Protection 78(11), 2089-2092.

Tada, J., Ohashi, T., Nishimura, N., Shirasaki, Y., Ozaki, H., N., Shirasaki, Y., Ozaki, H., Fukushima, S., Takano, J., Nishibuchi, M., & Takeda, Y. (1992). Detection of the thermostable direct hemolysin gene (tdh) and the thermostable direct hemolysin-related hemolysin gene (trh) of Vibrio parahaemolyticus by polymerase chain reaction. Molecular and Cellular Probes 6(6), 477–487.

Terai, A., Baba, K., Shirai, H., Yoshida, O., Takeda, Y., & Nishibuchi, M. (1991). Evidence for insertion sequence- mediated spread of the thermostable direct hemolysin gene among Vibrio species. Journal of Bacteriology 173(16), 5036-5046.

Thuy, H. T. T., Nga, L. P., & Loan, T. T. C. (2011). Antibiotic contaminants in coastal wetlands from Vietnamese shrimp farming. Environmental Science and Pollution Research 18, 835-841.

Tra, V. T. T., Meng, L., Pichpol, D., Pham, N. H., Baumann, M., Alter, T., & Huehn, S. (2016). Prevalence and antimicrobial resistance of Vibrio spp. in retail shrimps in Vietnam. Berliner und M ̈unchener tier ̈arztliche Wochenschrift 129(1-2), 48–51.

Tran, L., Nunan, L., Redman, R., Mohney, L., Pantoja, C., Fitzsimmons, K., & Lightner, D. (2013). Determination of the infectious nature of the agent of acute hepatopancreatic necrosis syndrome affecting penaeid shrimp. Diseases of Aquatic Organisms 105(1), 45-55.

Wong, M. H. Y., Liu, M., Wan, H. Y., & Chen, S. (2012). Characterization of Extended-Spectrum-β-Lactamase-Producing Vibrio parahaemolyticus. Antimicrobial Agents and Chemotherapy 56(7), 4026 - 4028.