Main Article Content
Response surface methodology was applied to optimize the extraction of phenolic compounds from fresh Pouzolzia zeylanica plant using hot water as a solvent. A central composite design (CCD) in form (23+star) was used to investigate the effects of two independent variables, namely, extraction temperature (70 to 90oC) and extraction time (20 to 40 min). The dependent variables were the content of anthocyanin, flavonoid, polyphenol, tannin and total soluble solids of extracted solution. A second-order polynomial model was used for predicting the response. The results showed that the optimal extraction process was obtained at 84.4oC for 31.7 min. The experimental values agreed with predicted within a 95% confidence interval. Consequently, the contents of anthocyanin, flavonoid, polyphenol and tannin were 38.66 mgCE/100 g, 3.01 mgQE/g, 5.17 mgGAE/g, 4.07 mgTAE/g fresh weight, and total soluble solids content was 0.73%, respectively.
Ahmed, J. K., Salih, H. A. M., & Hadi, A. G. (2013). Anthocyanin in red beet juice act as scavenger for heavy metals ions such as lead and cadmium. International Journal of Science and Technology 2(3), 269-273.
Al-Farsi, M. A., & Lee, C. Y. (2008). Optimization of phenolics and dietary fibre extraction from date seeds. Food Chemistry 108(3), 977-985. https://doi.org/10.1016/j.foodchem.2007.12.009
Chew, K. K., Khoo, M. Z., Ng, S. Y., Thoo, Y. Y., Wan Aida, W. M., & Ho, C. W. (2011). Effect of ethanol concentration, extraction time and extraction temperature on the recovery of phenolic compounds and antioxidant capacity of Orthosiphon stamineus extracts. International Food Research Journal 18(4), 1427-1435.
Connolly, D.L. (1993). Tannin extraction. US Patent 5, 238-680.
Eswari, M. L., Bharathi, R. V., & Jayshree, N. (2013). Preliminary phytochemical screening and heavy metal analysis of leaf extracts of Ziziphus oenoplia (L) Mill. Gard. International Journal of Pharmaceutical Sciences and Drug Research 5(1), 38-40.
Giang, H. T., Nguyen, H. T., Ngo, M. X., Nguyen, T. T. B., Pham, N. D., Nguyen, O. T., Phan, H. T., & Duez, P. (2013). Effects of raw material types on the chemical composition of Trung Du tea variety (Camellia sinensis var. sinensis). Journal Science & Development 11(3), 373-379.
Guan, X., & Yao, H. (2008). Optimization of viscozyme L assisted extraction of oat bran protein using response surface methodology. Food Chemistry 106(1), 345-351. https://doi.org/10.1016/j.foodchem.2007.05.041
Handa, S. S., Khanuja, S. P. S., Longo, G., & Rakesh, D. D. (2008). Extraction technologies for medicinal and aromatic plants (1st ed.). Trieste, Italy: United Nations Industrial Development Organization and the International Centre for Science and High Technology.
Hossain, M. A., AL-Raqmi, K. A. S., AL-Mijizy, Z. H., Weli, A. M., & Al-Riyami, Q. (2013). Study of total phenol, flavonoids contents and phytochemical sreening of various leaves crude extracts of locally grown Thymus vularis. Asian Pacific Journal of Tropical Biomedicine 3(9), 705-710. https://doi.org/10.1016/S2221-1691(13)60142-2
Laitonjam, W. S., Yumnam, R., Asem, S. D., & Wangkheirakpam, S. D. (2013). Evaluative and comparative study of biochemical, trace elements and antioxidant activity of Phlogacanthus pubinervius T. Anderson and Phlocanthus jenkincii C.B. Clarke leaves. Indian Journal of Natural Products and Resources 4(1), 67-72.
Le, T. N. (2003). Food Chemistry. Ha Noi, Vietnam: Publishing House of Science and Technology.
Mohammad, K., Hefazat, H. S. & Sheeba, F. (2011). Free radical scavenging and total phenolic content of Saccharum spontaneous L. root extracts. International Journal of Research in Pharmacy and Chemistry 1(4), 1160-1166.
Pinelo, M., Fabbro, P. D., Manzocco, L., Nunez, M. J., & Nicoli, M. C. (2005a). Optimization of continuous phenol extraction from vitisvinifera byproducts. Food Chemistry 92(1), 109-117. https://doi.org/10.1016/j.foodchem.2004.07.015
Pinelo, M., Rubilar, M., Jerez, M., Sineiro, J., & Nunez, M. J. (2005b). Effect of solvent, temperature, and solvent-to-solid ratio on the total phenolic content and antiradical activity of extracts from different components of grapepomace. Journal of Agricultural and Food Chemistry 53(6), 2111-2117. https://doi.org/10.1021/jf0488110
Rajha, H. N., Darra, N. E., Louka, N., Maroun, R. G., Ziegler, W., & Bochzelt, H. (2012). Valorization of industrial waste using energy saving procedures. Phenolic compounds purification from grape by-products by accelerated solvent extraction (ASE). International Conference on Renewable Energies for Developing Countries, 1-5. https://doi.org/10.1109/REDEC.2012.6416702.
Rajha, H. N., Louka, N., Darra, N. E., Hobaika, Z., Boussetta, N., Vorobiev, E., & Maroun, R. G. (2014). Multiple response optimization of high temperature, low time aqueous extraction process of phenolic compounds from grape byproducts. Food and Nutrition Sciences 5(4), 351-360. https://doi.org/10.4236/fns.2014.54042
Ramos, L., Kristenson, E. M., & Brinkman, U. A. (2002). Current use of pressurised liquid extraction and subcritical water extraction in environmental analysis. Journal of Chromatography A 975(1), 3-29. https://doi.org/10.1016/S0021-9673(02)01336-5
Saha, D., & Paul, S. (2012). Antifungal activity of ethanol extract of Pouzolzia Zeylanica (L.) Benn. International Journal of Pharmacy Teaching and Pratices 3(2), 272-274.
Saha, D., Paul, S., & Chowdhury, S. (2012). Antibacterial activity of ethanol extract of Pouzolzia Zeylanica (L.) Benn. International Journal of Pharmaceutical Innovations (IJPI) 2(1), 1-5.
Sheng, Z. L., Wan, P. F., Dong, C. L., & Li, Y. H. (2013). Optimization of total flavonoids content extracted from Flos populi using response surface Methodology. Industrial Crops and Products 43, 778-786. https://doi.org/10.1016/j.indcrop.2012.08.020
Spigno, G., & De-Faveri, D. M. (2007). Antioxidants from grape stalks and marc: influence of extraction procedure on yield, purity and antioxidant power of the extracts. Journal of Food Engineering 78(3), 793-801. https://doi.org/10.1016/j.jfoodeng.2005.11.020
Spigno, G., Tramelli, L., & De-Faveri, D. M. (2007). Effects of extraction time, temperature and solvent on concentration and antioxidant activity of grape marc phenolics. Journal of Food Engineering 81(1), 200-208. https://doi.org/10.1016/j.jfoodeng.2006.10.021
Tran, T. M., Nguyen, H. T. T., Duong, N. T. M., Tran, L. C., & La, K. V. (2010). Experimental study on some pharmacological effects of Pouzolzia zeylanica and Tinospora crispa. Medicine Journal of Ho Chi Minh City, Special Subject Traditional Medicine 14(2), 116-120.
Vo, C. V. (2012). Dictionary medicinal plants in Vietnam. Ha Noi, Vietnam: Medicine Publishing House.
Vu, S. H., & Ha, T. D. (2009). Study on polyphenol extraction from dust green tea. Part 1. The influence factors on polyphenol extraction. Journal of Science and Technology 47(1), 81-86.
Vyas, J., Itankar, P., Tauqeer, M., Kelkar, A., & Agrawal, M. (2013). Development of HPTLC method for estimation of piperine, guggulsterone E and Z in polyherbal formulation. Pharmacognosy Journal 5(6), 259-264. https://doi.org/10.1016/j.phcgj.2013.10.003
Yilmaz, Y., & Toledo, R. T. (2006). Oxygen radical absorbance capacities of grape/wine industry by products and effect of solvent type on extraction of grape seed polyphenols. Journal of Food Composition and Analysis 19(1), 41-48. https://doi.org/10.1016/j.jfca.2004.10.009
Zabeti, M., Daud, W. M. A. W., & Aroua, M. K. (2009). Optimization of the activity of CaO/Al2O3 catalyst for biodiesel production using response surface methodology. Applied Catalysic A: General 366(1), 154-159. https://doi.org/10.1016/j.apcata.2009.06.047