Ngoc T. A. Tong * , Tu C. Nguyen , & Ha C. Nguyen

* Correspondence: Tong Thi Anh Ngoc (email:

Main Article Content


The study aimed to screen the antibacterial activity of seven types of essential oils against 5 bacteria and 4 strains of E. coli using disk diffusion and MIC method. The essential oils were more effective against Gram-positive bacteria as compared to Gram-negative species. Thyme and cinnamon essential oils showed strong antimicrobial activity against all microorganisms tested. Especially, the tested essential oils inhibited the growth of multi-antibiotics resistant bacteria isolated from food processing chains, indicatingthe possibility of their potential use in reality. The MIC values of cinnamon and thyme essential oils against E. coli ATCC 25922, S. typhimurium, S. aureus, L. monocytogenes and P. aeruginosa were 512-1024 μg/mL and 1024-4096 μg/mL, respectively. The present study provided useful information and showed the potential use of cinnamon and thyme essential oils in food storage as natural antimicrobial preservatives.

Keywords: Antimicrobial, Bacteria, Disk-diffusion, Essential oils, MIC (Minimum Inhibitory Concentration), Pathogens

Article Details


Ajitha, B., Reddy, Y. A. K., & Reddy, P. S. (2014). Biosynthesis of silver nanoparticles using Plectranthus amboinicus leaf extract and its anti-microbial activity. Spectrochimica Acta Part A: Molecular and Biomolecular pectroscopy 128, 257-262.

Al-Mariri, A., & Safi, M. (2014). In vitro antibacterial activity of several plant extracts and oils against some gram-negative bacteria. Iranian Journal of Medical Sciences 39(1), 36-43.

Baydar, H., Sa˘gdi¸c, O., O¨ zkan, G., & Karadog˘an, T. (2004). Antibacterial activity and composition of essential oils from Origanum, Thymbra and Satureja species with commercial importance in Turkey. Food Control 15(3), 169-172.

Burt, S. (2004). Essential oils: their antibacterial properties and potential applications in foods - a review. International Journal of Food Microbiology 94(3), 223- 253.

Castilho, P. C., Savluchinske-Feio, S., Weinhold, T. S.,& Gouveia, S. C. (2012). Evaluation of the anti- microbial and anti-oxidant activities of essential oils, extracts and their main components from oregano from Madeira Island, Portugal. Food Control 23(2), 552-558.

Cetin, B., Cakmakci, S., & Cakmakci, R. (2011). The investigation of antimicrobial activity of thyme and oregano essential oils. Turkish Journal of Agriculture and Forestry 35, 145-154.

Chouhan, S., Sharma, K., & Guleria, S. (2017). Antimicrobial activity of some essential oils - present status and future perspectives. Medicines 4(3), 58.

CLSI (Clinical and Laboratory Standards Institute). (2019). Performance standards for antimicrobial disk susceptibility tests, approved standard (29th ed.) CLSI document M02-A11. Pennsylvania, USA: Clinical and Laboratory Standards Institute.

da Silva, F. T., da Cunha, K. F., Fonseca, L. M., Antunes, M. D., El Halal, S. L. M., Fiorentini, A. M., da Rosa Zavareze, E., & Dias, A. R. G. (2018). Action of ginger essential oil (Zingiber officinale) encapsulated in proteins ultrafine fibers on the antimicrobial control in situ. International Journal of Biological Macromolecules 118(A),107-115.

Desam, N. R., Al-Rajab, A. J., Sharma, M., Mylabathula,M. M., Gowkanapalli, R. R., & Albratty, M. (2017). Chemical constituents, in vitro antibacterial and antifungal activity of Mentha x Piperita L. (peppermint) essential oils. Journal of King Saud University-Science 31(4), 528-533.

Dorman, H. J. D., & Deans, S. G. (2000). Antimicrobial agents from plants: antibacterial activity of plant volatile oils. Journal of Applied Microbiology 88(2), 308-316.

Hassani, M. S., Zainati, I., Zrira, S., Mahdi, S., & Oukessou, M. (2012). Chemical composition and antimicrobial activity of Plectranthus amboinicus (Lour) Spring. Essential oil from archipelago of Comoros. Journal of Essential Oil Bearing Plants 15(4), 637-644.

Judith, V., Luis, B. R., Tulia, D. & Alfredo, U. (2013). Chemical composition and antibacterial activity of the essential oil of Coleus amboinicus Lour., against enteric pathogens. Journal of Essential Oil Bearing Plants 12(4), 453-461.

Kalemba, D., & Synowiec, A. (2020). Agrobiological in- teractions of essential oils of two menthol mints: Mentha piperita and mentha arvensis. Molecules 25(1), 59.

Keskin, D., & Toroglu, S. (2011). Studies on antimicrobial activities of solvent extracts of different spices. Journal of Environmental Biology 32(2), 251-256.

Kin, A., Yaki, L. M., Abubakar, I., Olusola, L. F., & Zubairu, R. (2018). Antibacterial activity of Ocimum gratissimum (scent leaf) on some pathogenic gastrointestinal bacteria. African Journal of Microbiology Research 12(40), 923-929.

Kordali, S., Kotan, R., Mavi, A., Cakir, A., Ala, A.,& Yildirim, A. (2005). Determination of the chemi- cal composition and antioxidant activity of the essential oil of Artemisia dracunculus and of the anti-fungal and anti-bacterial activities of Turkish Artemisia absinthium, A. dracunculus, Artemisia santonicum, and Artemisia spicigera essential oils. Journal of Agricultural and Food Chemistry 53(24), 9452-9458.

Kumar, S., & Kumari, R. (2019). Cinnamomum: Review article of essential oil compounds, ethnobotany, anti-fungal and antibacterial effects. Open Access Journal of Science 3(1), 13-16.

La Storia, A., Ercolini, D., Marinello, F., Di Pasqua, R., Villani, F., & Mauriello, G. (2011). Atomic force microscopy analysis shows surface structure changes in carvacrol-treated bacterial cells. Research in microbiology 162(2), 164-172.

Magi, G., Marini, E., & Facinelli, B. (2015). Antimicrobial activity of essential oils and carvacrol, and synergy of carvacrol and erythromycin, against clinical, erythromycin-resistant Group A Streptococci. Frontiers in microbiology 6, 165.

Marta War, O., Rodriguez, M., Garcia, G., & CeliaLierene, R. (2004). Antimicrobial activity of the essential oil and cream of Cymbopogon citratus (DC.) stapf. Revcubana Plt Med 2, 44-47.

Matasyoh, L. G., Matasyoh, J. C., Wachira, F. N., Kinyua, M. G., Muigai, A. W. T., & Mukiama, T. K. (2008). Anti-microbial activity of essential oils of Ocimum gratissimum L. from different populations of Kenya. African Journal of Traditional, Complementary and Alternative Medicines 5(2), 187-193.

Melo, A. D. B., Amaral, A. F., Schaefer, G., Luciano, F. B., de Andrade, C., Costa, L. B., & Rostagno, M. H. (2015). Anti-microbial effect against different bacterial strains and bacterial adaptation to essential oils used as feed additives. Canadian Journal of Veterinary Research 79(4), 285-289.

Mith, H., Dure, R., Delcenserie, V., Zhiri, A., Daube, G., & Clinquart, A. (2014). Antimicrobial activities of commercial essential oils and their components against food-borne pathogens and food spoilage bacteria. Food Science & Nutrition 2(4), 403-416.

Nguyen, L. T. H., Bui, T. Q., Le, T. D., & Nguyen, D.T. N. (2015). Study on the Anti-bacterial Activity of Essential Oils from Perilla Leaves. Journal of Science and Development 13, 245-250.

Nikaido, H. (2003). Molecular basis of bacterial outer membrane permeability revisited. Microbiology and Molecular Biology Reviews 67, 593-656.

Oussalah, M., Caillet, S., Saucier, L., & Lacroix, M. (2007). Inhibitory effects of selected plant essential oils on the growth of four pathogenic bacteria: E. coli O157: H7, Salmonella typhimurium, Staphylococcus aureus and Listeria monocytogenes. Food Control 18(5), 414-420.

Pereira, R. S., Sumita, T. C., Furlan, M. R., Jorge, A. O. C., & Ueno, M. (2004). Antibacterial activity of essential oils on microorganisms isolated from urinary tract infection. Revista de Saude Publica 38(2), 326-328.

Shan, B., Cai, Y. Z., Brooks, J. D., & Corke, H. (2007). Antibacterial properties and major bioactive components of cinnamon stick (Cinnamomum burmannii): activity against foodborne pathogenic bacteria. Journal of Agricultural and Food Chemistry 55(14), 5484-5490.

Srisukh, V., Tribuddharat, C., Nukoolkarn, V., Bunyapraphatsara, N., Chokephaibulkit, K., Phoom- niyom, S., Chuanphung, S., & Srifuengfung, S. (2012). Antibacterial activity of essential oils from Citrus hystrix (makrut lime) against respiratory tract pathogens. Science Asia 38, 212-217.

Subramaniam, G., Yew, X. Y., & Sivasamugham, L. A. (2020). Antibacterial activity of Cymbopogon citratus against clinically important bacteria. South African Journal of Chemical Engineering 34, 26-30.

Tayel, A. A., El-Tras, W. F., Moussa, S. H., & El-Sabbagh, S. M. (2012). Surface decontamina- tion and quality enhancement in meat steaks using plant extracts as natural biopreservatives. Foodborne Pathogens and Disease 9(8), 755-761.

Trombetta, D., Castelli, F., Sarpietro, M. G., Venuti, V., Cristani, M., Daniele, C., Saija, A., Mazzanti, G., & Bisignano, G. (2005). Mechanisms of antibacterial action of three monoterpenes. Antimicrobial Agents and Chemotherapy 49(6), 2474-2478.

Van de Vel, E., Sampers, I., & Raes, K. (2019). A review on influencing factors on the minimum inhibitory con- centration of essential oils. Critical Reviews in Food Science and Nutrition 59(3), 357-378.

Vu, T. T., & Nguyen, H. T. (2015). Inhibitions of Staphylococcus aureus by using the combination of Viet- namese essential oils. Vietnam Journal of Science and Technology 53(4), 417-424.