Han M. Ly * , Trinh T. K. Nguyen , Thiep T. X. Dang , & An T. T. Vo

* Correspondence: Ly Mai Han (email: 14112089@st.hcmuaf.edu.vn)

Main Article Content


The polymerase chain reaction (PCR) for Actinobacillus pleuropneumonia (App), Haemophilus parasuis (Hps), Pasteurella multocida (Pm) and Bordetella bronchiseptica (Bb) were performed in pure colonies isolated  from 114 lung specimens  with lesions collected from the Vissan slaughterhouse  in Ho Chi Minh City from July 2018 to May 2019. The aim of the experiment was to identify the four respiratory pathogenic bacteria in pigs at slaughterhouse by using PCR technique. The criteria for evaluating the results  included the proportion of positive samples with multiplex PCR and percentage of samples co-infected with 2, 3, and 4 bacteria. Among a total of 114 injured lung samples, 21% of the samples was positive to at least one of the four bacteria, 3 samples (2.63%) were positive for App, 2 samples (1.75%) were positive for Hps, 7 samples (6.14%) were for Pm, and 12 lungs (10.53%) were positive for Bb. One sample (0.88%) was found co-infected with Pm and Hps.

Keywords: multiplex polymerase chain reaction (PCR), pig lungs, respiratory bacteria, slaughterhouse

Article Details


de Jong, A., Thomas, V., Simjee, S., Moyaert, H., Garch, F. E., Maher, K., Morrissey, I., Butty, P., Klein, U., Marion, H., Rigaut, D., & Vallé, M. (2014). Antimicrobial susceptibility monitoring of respiratory tract pathogens isolated from diseased cattle and pigs across Europe: The VetPath study. Veterinary Microbiology 172(1-2), 202-215. https://doi.org/10.1016/j.vetmic.2014.04.008

Hozbor, D., Fouque, F., & Guiso, N. (1999). Detection of Bordetella bronchiseptica by the polymerase chain reaction. Research in Microbiology 150(5), 333-341. https://doi.org/10.1016/S0923-2508(99)80059-X

Hričínová, M., Holoda, E., Mudroňová, D., & Ondrašovičová, S. (2010). Multiplex PCR assay for detection of Actinobacillus pleuropneumoniae , Pasteurella multocida and Haemophilus parasuis in lungs of pigs from a slaughterhouse. Folia Microbiologica 55(6), 635- 640. https://doi.org/10.1007/s12223-010-0103-9

Kumar, S., Singh, B.R., Bhardwaj, M., & Singh, V. (2014). Occurrence of Bordetella infection in pigs in Northern India. International Journal of Microbiology 2014, 1-6. https://doi.org/10.1155/2014/238575

Oliveira, S., Galina, L., & Pijoan, C. (2001). Development of a PCR test to diagnose Haemophilus parasuis infections. Journal of Veterinary Diagnostic Investigation 13(6), 495-501. https://doi.org/10.1177/104063870101300607

Townsend, K. M., Frost, A. J., Lee, C. W., Papadimitriou, J. M., & Dawkins, H. J. S. (1998). Development of PCR assays for species- and type-specific identification of Pasteurella multocida Isolates. Journal of Clinical Microbiology 36(4), 1096-1100. https://doi.org/10.1128/JCM.36.4.1096-1100.1998

Truong, L. Q., Nguyen, L. T., Nguyen, H. T., & Nguyen, H. T. (2018). Isolation and serotyping of Haemophilus parasuis trains from pigs raised in the provinces of Thanh Hoa, Hung Yen, and Ha Nam of Northern Vietnam. Vietnam Journal of Agricultural Sciences 16(12), 1068-1078.

Le, D. V., Nguyen, T. Q., Cu, P. H., & Hoang, H. D. (2012). Results of isolation and determination of some biological characteristics of Pasteurella multocida in pigs positive with PRRSV at Bac Giang Province. Veterinary Sciences and Techniques 19(6), 47-51.

Xiao, G. S., Cao, S. J., Duan, L. L., Wen, X. T., Ma, X. P., & Chen, H. M. (2006). Identification and detection of Actinobacillus pleuropneumoniae in Infected and subclinically infected pigs by multiplex PCR based on the genes ApxIVA and OmlA. Agricultural Sciences in China 5(2), 146-154. https://doi.org/10.1016/S1671-2927(06)60032-1

Zhao, Z., Wang, C., Xue, Y., Tang, X., Wu, B., Cheng, X., He, Q., & Chen, H. (2011). The occurrence of Bordetella bronchiseptica in pigs with clinical respiratory disease. The Veterinary Journal 188(3), 337-340. https://doi.org/10.1016/j.tvjl.2010.05.022