Han M. Ly * , Trinh T. K. Nguyen , Thiep T. X. Dang , & An T. T. Vo

* Correspondence: Ly Mai Han (email: 14112089@st.hcmuaf.edu.vn)

Main Article Content

Abstract

The polymerase chain reaction (PCR) for Actinobacillus pleuropneumonia (App), Haemophilus parasuis (Hps), Pasteurella multocida (Pm) and Bordetella bronchiseptica (Bb) were performed in pure colonies isolated  from 114 lung specimens  with lesions collected from the Vissan slaughterhouse  in Ho Chi Minh City from July 2018 to May 2019. The aim of the experiment was to identify the four respiratory pathogenic bacteria in pigs at slaughterhouse by using PCR technique. The criteria for evaluating the results  included the proportion of positive samples with multiplex PCR and percentage of samples co-infected with 2, 3, and 4 bacteria. Among a total of 114 injured lung samples, 21% of the samples was positive to at least one of the four bacteria, 3 samples (2.63%) were positive for App, 2 samples (1.75%) were positive for Hps, 7 samples (6.14%) were for Pm, and 12 lungs (10.53%) were positive for Bb. One sample (0.88%) was found co-infected with Pm and Hps.

Keywords: multiplex polymerase chain reaction (PCR), pig lungs, respiratory bacteria, slaughterhouse

Article Details

References

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