Effects of gamma rays from 60Co source on dwarf Dendrobium cross-breeding
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Abstract
In this research, seeds of DM12x13 dwarf Dendrobium cross-breeding germinated on 1/2 MS medium after 40 days were irradiated by 60Co source with various doses to determine LD50 (Lethal Dose 50%). After LD50 had been determined, protocorms of DM12x13 dwarf Dendrobium cross-breeding was irradiated by gamma rays from 60Co source in 5 doses: 0 Gy (control), 20 Gy, 40 Gy, 60 Gy and 80 Gy, dose rate 90 Gy/h to study the effect of gamma radiation on mutagenicity and evaluate the growth of these mutations in vitro. The result showed that the lethal dose of hybrid DM12x13 Dendrobium protocorm under the effect of 60Co gamma ray was 68 Gy. Survival ratio, growth and the ability to growth of plants reduced in higher doses and dead ratio were 100% when irradiated more than 80 Gy after 7 months. The effective doses for treating protocorm of DM12x13 crossbred lines were 20, 40 and 60 Gy. These doses were suitable for increased frequency of variation with generating wide-spread, diverse in structure and color of stems and leaves.
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