Dieu M. T. Nguyen , Phuong H. T. Vo , Do N. Nguyen , Nghi N. Duong , & Phuong H. Le *

* Correspondence: Le Hong Phuong (email: phuonghle@hcmuaf.edu.vn)

Main Article Content

Abstract

This study was conducted to evaluate the use of pectinase to degrade pectin in cocoa mesocarp. A completely randomized design with two factors was used: enzyme concentration (0.5, 1, & 3%) and treatment time (30, 60, 90, and 120 min). The results showed that increasing both enzyme concentration and treatment time significantly decreased the pectin content in cocoa mesocarp, from 4.86 to 0.08%. Similarly, viscosity decreased with increasing enzyme concentration and treatment time. Pectin was greatly degraded when treated with a 3% enzyme concentration for 120 min. These findings underscore the potential of pectinase to modify the properties of cocoa pod husk, which can be used to develop high-value products, contributing to maximize the utilization of this agricultural by-product.

Keywords: Mesocarp cocoa, Pectinase, Treatment efficiency, Viscosity

Article Details

References

Afoakwa, E. O. (2014). Cocoa production and processing technology (1st ed.). London, England: CRC Press. https://doi.org/10.1201/b16546.

Barišić, V., Jozinović, A., Flanjak, I., Šubarić, D., Babić, J., Miličević, B., Doko, K., & Ačkar, Đ. (2020). Difficulties with use of cocoa bean shell in food production and high voltage electrical discharge as a possible solution. Sustainability 12(10), 3981. https://doi.org/10.3390/su12103981.

Blakemore, W. R., Dewar, E. T., & Hodge, R. A. (1966). Polysaccharides of the cocoa pod husk. Journal of The Science of Food and Agriculture 17(12), 558-560. https://doi.org/10.1002/jsfa.2740171208.

Chan, S. Y., & Choo, W. S. (2013). Effect of extraction conditions on the yield and chemical properties of pectin from cocoa husks. Food Chemistry 141(4), 3752-3758.

Dos Santos, E. S. B., Leal, I. L., & Nery, T. B. R. (2021). Extraction of pectin from epicarp and mesocarp fractions of cocoa shell. Journal of Bioengineering, Technologies and Health 4(2), 52-57. https://doi.org/10.1016/j.foodchem.2013.06.097.

Handojo, L., Triharyogi, H., & Indarto, A. (2019). Cocoa bean shell waste as potential raw material for dietary fiber powder. International Journal of Recycling of Organic Waste in Agriculture 8, 485-491. https://doi.org/10.34178/JBTH.V4I2.160.

Hoang, B. Q., Duong, D. T. N., Le, N. T., & Mai, T. T. (2020). Study on hydrolysis conditions of redfleshed dragon fruit juice (Hylocereus polyrhizus) and alcohol fermentation testing. Can Tho University Journal of Science 56(3): 86-92. https://doi.org/10.22144/ctu.jvn.2020.057.

Horwitz, W. (2010). Official methods of analysis of AOAC International. Agricultural chemicals, contaminants, drugs (Volume I). Maryland, USA: AOAC International.

Jong, S. H., Abdullah, N., & Muhammad, N. (2023). Optimization of low-methoxyl pectin extraction from durian rinds and its physicochemical characterization. Carbohydrate Polymer Technologies and Applications 5, 100263. https://doi.org/10.1016/j.carpta.2022.100263.

Jozinović, A., Panak Balentić, J., Ačkar, Đ., Babić, J., Pajin, B., Miličević, B., Guberac, S., Vrdoljak, A., & Šubarić, D. (2019). Cocoa husk application in the enrichment of extruded snack products. Journal of Food Processing and Preservation 43(2), e13866. https://doi.org/10.1111/jfpp.13866.

Lu, F., Rodriguez-Garcia, J., Van Damme, I., Westwood, N. J., Shaw, L., Robinson, J. S., Warren, G., Chatzifragkou, A., McQueen, M. S., Gomez, L., Faas, L., Balcombe, K., Srinivasan, C., Picchioni, F., Hadley, P., & Charalampopoulos, D. (2018). Valorization strategies for cocoa pod husk and its fractions. Current Opinion in Green and Sustainable Chemistry 14, 80-88. https://doi.org/10.1016/j.cogsc.2018.07.007.

Mai, T. T. N., Nguyen, V. V. T., Nguyen, H. C & Le, D. N. D. (2016). Study on factors affecting the ability to extract acerola fruit juice (Magnolyophyta glabra) by enzyme. Can Tho University Journal of Science (42), 11-18. https://doi.org/10.22144/ctu.jvn.2016.011.

Martínez, C. S., Salvador, A., Muguerza, B., Moulay, L., & Fiszman, S. (2011). Cocoa fibre and its application as a fat replacer in chocolate muffins. LWT-Food Science and Technology 44(3), 729-736. https://doi.org/10.1016/j.lwt.2010.06.035.

Mulluye, K., & Atnafu, Y. (2022). Biotechnological application of pectinase. International Journal of Pharmaceutical Sciences and Research 13(8), 3069-3077. http://dx.doi.org/10.13040/IJPSR.0975-8232.13(8).3069-77.

Nguyen, Q. N., Tran, K. T., & Vu, D. X. (2022). Research on the production and testing of the ability to preserve some fruits of pectin-carboxymethyl cellulose film supplemented with herbal essential oils. Hung Vuong University Journal of Science and Technology 26(1), 28-37.

Nguyen, T. H., & Luu, H. Đ. T. (2014). Research on cocoa product value chain in Ben Tre province Can Tho University Journal of Science (35), 8-15.

Oddoye, E. O., Agyente-Badu, C. K., & GyeduAkoto, E. (2013). Cocoa and its by-products: Identification and utilization. Chocolate in Health and Nutrition 7, 23-37. https://doi.org/10.1007/978-1-61779-803-03.

Pinkaew, T., Inthachat, W., Khemthong, C., Kemsawasd, V., On-Nom, N., & Temviriyanukul, P. (2024). High pectin recovery from cocoa husks using an autoclave approach: An analysis of its physicochemical, structural, and genotoxicity properties. Foods 13(5), 669. https://doi.org/10.3390/foods13050669.

Potanin, A., & Marron, G. (2021). Rheological characterization of yield-stress fluids with Brookfield viscometer. De Gruyter 31, 1-9.

Sobamiwa, O., & Longe, O. (1994). Utilization of cocoa-pod pericarp fractions in broiler chick diets. Animal Feed Science and Technology 47(3-4), 237-244. https://doi.org/10.1016/0377-8401(94)90127-9.

Tapre, A. R., & Jain, R. K. (2014). Pectinases: Enzymes for fruit processing industry. International Food Research Journal 21(2), 447-453.