Emissions of gases during bio-conversion of agro-waste by black soldier fly larvae
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Abstract
This laboratory-scale study was designed to investigate the emissions of carbon dioxide (CO2), ammonia (NH3), and hydrogen sulfide (H2S) gases during the bio-conversion of agro-waste by black soldier fly larvae (BSFL) for 14 days. The study included three experimental treatments: a control group without waste and BSFL (T0, lab background), treatment 1 containing waste with BSFL (T1), and treatment 2 containing only waste (without BSFL, T2). Process efficiency was measured by waste reduction and bio-conversion rate. Gas emissions from the process were collected using the static chamber method and determined using the gas absorption method. The results in the treatment T1 showed a notable BSFL survival rate of 99.7%, indicating a favorable condition for BSFL growth. The waste reduction rate in the T1 treatment (74.3%) was approximately two times higher than that of T2 (38.7%), indicating the ability of BSFL to decompose organic wastes efficiently. The pH and moisture content of the waste were monitored throughout the 14-day trial for both T1 and T2, and similar trends were observed. Compared to T0, gas emissions from T1 and T2 were higher. Furthermore, the CO2 and H2S emissions in T1 were higher than those in T2, while NH3 levels released in T2 were relatively higher than in T1. The preliminary results presented here could be the basis of future studies on gas emission via BSFL treatment of agro-waste.
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References
Arabzadeh, G., Delisle-Houde, M., Tweddell, R. J., Deschamps, M. H., Dorais, M., Lebeuf, Y., Derome, N., & Vandenberg, G. (2022). Diet composition influences growth performance, bioconversion of black soldier fly larvae: agronomic value and in vitro biofungicidal activity of derived frass. Agronomy 12(8), 1765. https://doi.org/10.3390/agronomy12081765.
Barrington, S., Choinière, D., Trigui, M., & Knight, W. (2002). Effect of carbon source on compost nitrogen and carbon losses. Bioresource Technology 83(3), 189-194. https://doi.org/10.1016/S0960-8524(01)00229-2.
Boakye-Yiadom, K. A., Ilari, A., & Duca, D. (2022). Greenhouse gas emissions and life cycle assessment on the black soldier fly (Hermetia illucens L.). Sustainability 14(16), 10456. https://doi.org/10.3390/su141610456.
Coudron, C. L., Berrens, S., Van Peer, M., Deruytter, D., Claeys, J., & Van Miert, S. (2024). Ammonia emissions related to black soldier fly larvae during growth on different diets. Journal of Insects as Food and Feed 10(2024), 1469-1483. https://doi.org/10.1163/23524588-00001049.
Chen, J., Hou, D., Pang, W., Nowar, E. E., Tomberlin, J. K., Hu, R., Chen, H., Xie, J., Zhang, J., Yu, Z., & Li, Q. (2019). Effect of moisture content on greenhouse gas and NH3 emissions from pig manure converted by black soldier fly. Science of The Total Environment 697, 133840. https://doi.org/10.1016/j.scitotenv.2019.133840.
Ebeneezar, S., Tejpal, C. S., Jeena, N. S., Summaya, R., Chandrasekar, S., Sayooj, P., & Vijayagopal, P. (2021). Nutritional evaluation, bioconversion performance and phylogenetic assessment of black soldier fly (Hermetia illucens, Linn. 1758) larvae valorized from food waste. Environmental Technology and Innovation 23, 101783. https://doi.org/10.1016/j.eti.2021.101783.
Giannetto, A., Oliva, S., Ceccon Lanes, C. F., de Araújo Pedron, F., Savastano, D., Baviera, C., Parrino, V., Lo Paro, G., Spanò, N. C., Cappello, T., Maisano, M., Mauceri, A., & Fasulo, S. (2020). Hermetia illucens (Diptera: Stratiomydae) larvae and prepupae: Biomass production, fatty acid profile and expression of key genes involved in lipid metabolism. Journal of Biotechnology 307, 44-54. https://doi.org/10.1016/j.jbiotec.2019.10.015.
Guo, R., Li, G., Jiang, T., Schuchardt, F., Chen, T., Zhao, Y., & Shen, Y. (2012). Effect of aeration rate, C/N ratio and moisture content on the stability and maturity of compost. Bioresource Technology 112, 171-178. https://doi.org/10.1016/j.biortech.2012.02.099.
GV (The Government of Vietnam). (2022). Decree No. 46/2022/ND-CP dated on July 13, 2022: Amendments to some articles of the Government’s Decree No. 13/2020/ND-CP dated January 21, 2020 on elaboration of the law on animal husbandry. Ha Noi, Vietnam. Retrieved June 1, 2024, from https://datafiles.chinhphu.vn/cpp/files/vbpq/2022/07/46-nd.signed.pdf.
Huis, A. V. (2020). Insects as food and feed, a new emerging agricultural sector: a review. Journal of Insects as Food and Feed 6(1), 27-44. https://doi.org/10.3920/JIFF2019.0017.
Jiang, T., Schuchardt, F., Li, G. X., Guo, R., & Luo, Y. M. (2013). Gaseous emission during the composting of pig feces from Chinese Ganqinfen system. Chemosphere 90(4), 1545-1551. https://doi.org/10.1016/j.chemosphere.2012.08.056.
Koerkamp, P. W. G. G. (1994). Review on emissions of ammonia from housing systems for laying hens in relation to sources, processes, building design and manure handling. Journal of Agricultural Engineering Research 59(2), 73-87. https://doi.org/10.1006/jaer.1994.1065.
Lalander, C., Diener, S., Zurbrügg, C., & Vinnerås, B. (2019). Effects of feedstock on larval development and process efficiency in waste treatment with black soldier fly (Hermetia illucens). Journal of Cleaner Production 208, 211-219. https://doi.org/10.1016/j.jclepro.2018.10.017.
Lindberg, L., Ermolaev, E., Vinnerås, B., & Lalander, C. (2022). Process efficiency and greenhouse gas emissions in black soldier fly larvae composting of fruit and vegetable waste with and without pre-treatment. Journal of Cleaner Production 338, 130552. https://doi.org/10.1016/j.jclepro.2022.130552.
Lopes, I. G., Lalander, C., Vidotti, R. M., & Vinnerås, B. (2020). Using Hermetia illucens larvae to process biowaste from aquaculture production. Journal of Cleaner Production 251, 119753. https://doi.org/10.1016/j.jclepro.2019.119753.
Ma, J., Lei, Y., Rehman, K. u., Yu, Z., Zhang, J., Li, W., Li, Q., Tomberlin, J. K., & Zheng, L. (2018). Dynamic effects of initial pH of substrate on biological growth and metamorphosis of black soldier fly (Diptera: Stratiomyidae). Environmental Entomology 47(1), 159-165. https://doi.org/10.1093/ee/nvx186.
Meneguz, M., Gasco, L., & Tomberlin, J. K. (2018). Impact of pH and feeding system on black soldier fly (Hermetia illucens, L; Diptera: Stratiomyidae) larval development. PLos One 13(8), e0202591-e0202591. https://doi.org/10.1371%2Fjournal.pone.0202591.
Michishita, R., Shimoda, M., Furukawa, S., & Uehara, T. (2023). Inoculation with black soldier fly larvae alters the microbiome and volatile organic compound profile of decomposing food waste. Scientific Reports 13(1), 4297. https://doi.org/10.1038/s41598-023-31388-z.
MOH (Ministry of Health). (1989). Branch standard No. 52 TCN 353-89/BYT: Air quality - Baryta absorption method for determination of carbon dioxide content. Retrieved June 20, 2024, from https://caselaw.vn/van-ban-phap-luat/259575-tieu-chuan-nganh-52-tcn-352-1989-vecacbon-oxyt-nam-1989-tinh-trang-hieu-luckhong-xac-dinh.
Nguyen, T. L., & Cao, T. S. (2024). Current situation of agricultural by-products and management solutions in Vietnam. In Proceedings of Agricultural and Rural Environment and Sustainable Development (159-168). Ha Noi, Vietnam: Vietnam National University of Agriculture.
Oonincx, D. G. A. B., van Broekhoven, S., van Huis, A., & van Loon, J. J. A. (2015). Feed conversion, survival and development, and composition of four insect species on diets composed of food by-products. PLos One 10 (12), e0144601. https://doi.org/10.1371/journal.pone.0144601.
Pang, W., Hou, D., Chen, J., Nowar, E. E., Li, Z., Hu, R., Tomberlin, J. K., Yu, Z., Li, Q., & Wang, S. (2020a). Reducing greenhouse gas emissions and enhancing carbon and nitrogen conversion in food wastes by the black soldier fly. Journal of Environmental Management 260, 110066. https://doi.org/10.1016/j.jenvman.2020.110066.
Pang, W., Hou, D., Nowar, E. E., Chen, H., Zhang, J., Zhang, G., Li, Q., & Wang, S. (2020b). The influence on carbon, nitrogen recycling, and greenhouse gas emissions under different C/N ratios by black soldier fly. Environmental Science and Pollution Research 27(34), 42767-42777. https://doi.org/10.1007/s11356-020-09909-4.
RIBE (Research Institute for Biotechnology and Environment). (2017). Research on black soldier fly - feeding and management in Vietnam (research report). Nong Lam University, Ho Chi Minh City, Vietnam.
VS (Vietnam Standards). (1995). Standard No. TCVN 5293 : 1995: Air quality - Indophenol method for determination of ammonia content. Retrieved June 20, 2024, from https://caselaw.vn/van-ban-phap-luat/258473-tieu-chuan-vietnam-tcvn-5293-1995-st-sev-5299-80-ve-chatluong-khong-khi-phuong-phap-indophenolxac-dinh-ham-luong-amoniac-nam-1995.
Wang, Y. S., & Shelomi, M. (2017). Review of black soldier fly (Hermetia illucens) as animal feed and human food. Foods 6(10), 91. https://doi.org/10.3390/foods6100091.
WHO (World Health Organization). (1981). Hydrogen sulfide. Geneva, Switzerland: IPCS International Programme on Chemical Safety.
Yang, F., Li, G., Shi, H., & Wang, Y. (2015). Effects of phosphogypsum and superphosphate on compost maturity and gaseous emissions during kitchen waste composting. Waste Management 36, 70-76. https://doi.org/10.1016/j.wasman.2014.11.012.
Yang, S. (2019). Explanation of an industrial application of black soldier fly converting food waste into valuable products. Retrieved June 22, 2024, from https://www.evoconsys.com/blog/explanation-of-an-industrial-application-ofblack-soldier-fly-converting-food-waste-intovaluable-products.
Zhang, H., Schuchardt, F., Li, G., Yang, J., & Yang, Q. (2013). Emission of volatile sulfur compounds during composting of municipal solid waste (MSW). Waste Management 33(4), 957-963. https://doi.org/10.1016/j.wasman.2012.11.008.
Zhang, X., Li, Z., Nowar, E. E., Chen, J., Pang, W., Hou, D., Hu, R., Jiang, H., Zhang, J., & Li, Q. (2021). Effect of batch feeding times on greenhouse gas and NH 3 emissions during meat and bone meal bioconversion by black soldier fly larvae. Waste and Biomass Valorization 12(7), 3889-3897. https://doi.org/10.1007/s12649-020-01277-x.