Assessing the degradation potential of pineapple stems and leaves by Trichoderma spp.
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Abstract
The study aimed to determine suitable substrate ratios and humidities to produce organic fertilizer from the stems and leaves of pineapples using Trichoderma spp. strains (TC). A completely randomized 6 x 3 factorial design was used. The factor (A) was the different supplementation of cellulose-degrading fungi (no supplementation, Tricho-DHCT, TC1, TC2, TC3, and a mixture of TC1, TC2, and TC3). The factor (B) was three levels of humidity (50%, 60%, and 70%). The experiment used 3 different substrate ratios (pineapple stem leaves, sugarcane filter cake, and cow dung corresponding to 7:1.5:1.5 (KBB7); 8:1:1 (KBB8); and 9:0.5:0.5 (KBB9)). The results showed that the substrate ratios KBB7, KBB8, and KBB9 supplemented with the mixed Trichoderma strains (TC1 + TC2 + TC3) exhibited a greater total P content than the treatments supplemented with individual strains (0.574% vs. 0.389 - 0.463% for KBB7, 0.507% vs. 0.351 - 0.380% for KBB8, and 0.422% vs. 0.303 - 0.427%, respectively). Likewise, in all three substrate ratios, KBB7, KBB8, and KBB9, the control treatment had a greater C/N ratio than the other treatments supplemented with Trichoderma spp. or Tricho-DHCT (43.8 vs. 28.3 - 35.1 for KBB7, 47.8 vs. 31.6 - 35.2 for KBB8, and 54.8 vs. 34.2 - 37.1 for KBB9, respectively). The total C content also showed a similar trend. In general, the substrate ratio of 7:1.5:1.5 at a humidity of 50 or 60% is suitable to produce organic fertilizer.
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