Effect of growth regulators and culture conditions for micropropagation of Vietnamese ginseng (Panax vietnamensis Ha et Grushv) on a temporary immersion system
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Abstract
Ngoc Linh ginseng is a valuable medicinal crop recognized for its anti-amnesia, anti-inflammatory, hepatoprotective, and anti-cancer properties. This study was to investigate the variation effect of growth regulators and culture conditions for micropropagation of Ngoc Linh ginseng (Panax vietnamensis Ha et Grushv.) on a temporary immersion system. After ten weeks of cultivation, the best callus induction rate in a solid Murashige and Skoog (MS) medium containing 2,4-dichlorophenoxyacetic acid (2,4-D) 1.5 mg/L was 90%. Callus cultured on MS medium (indole-3-butyric acid (IBA) 0.7 mg/L) supplemented with Thidiazuron (TDZ) 0.8 mg/L resulted in the highest embryogenesis rate, reaching 69.29%, with an average of 11.25 embryos per explant. The MS medium supplemented with 6-benzylaminopurine (BAP) 1 mg/L and α-naphthalene acetic acid (NAA) 1 mg/L was shown to be appropriate for shoot growth from Ngoc Linh ginseng embryos. The temporary immersion system showed that with a 6-benzyladenine acid (BA) concentration of 1.5 mg/L for 3 min of immersion, the greatest value for shoot number was 6.00 shoots/explant, and the height was 2.70 cm. Roots developed best at a treatment with a 5 min of immersion and NAA 1 mg/L concentration, root formation frequency, and root length reaching values of (71.25%; 1.61 cm). As a result, the temporary immersion system (TIS) system can be used for high-efficiency in vitro propagation of Ngoc Linh ginseng plants.
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References
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